TAMRA

[Maximiliano Neme]

Hello,

I’ve been asked about whether is a good option or not to use TAMRA (Rhodamine derived) for labeling DNA in a confocal microscope (HeNe 543 laser) taking into account its behavior regarding bleaching.

Is it relatively stable with time/light exposure?

Any advice will be very welcomed.

Thanks

______________________________________
Ing. Maximiliano Neme
Coordinador del área de imágenes
Fundación Instituto Leloir 
www.leloir.org.ar

 

[Vitaly Boyko]

Hi Maximiliano,

 

It is OK.

 

I suppose someone will be using DNA or RNA oligos for the DNA labeling. I would suggest to have 2+ TAMRA labeled nucleotides per oligo, at least 12 nts apart. It could be expensive when asking a company to make it for you, but if someone has a capability to synthesize the oligo in-house it will be much cheaper, and one can incorporate four TAMRA labeled nts within the 55-60 nts long oligo. RNA oligos are more expensive but work better than the DNA ones.

 

Good luck,

 

Vitaly

[phone]

 

---

From: Maximiliano Neme <[hidden email]>
To: [hidden email]
Sent: Wed, February 17, 2010 2:41:43 PM
Subject: TAMRA

Hello,

I’ve been asked about whether is a good option or not to use TAMRA (Rhodamine derived) for labeling DNA in a confocal microscope (HeNe 543 laser) taking into account its behavior regarding bleaching.

Is it relatively stable with time/light exposure?

Any advice will be very welcomed.

Thanks

______________________________________
Ing. Maximiliano Neme
Coordinador del área de imágenes
Fundación Instituto Leloir 
www.leloir.org.ar