TIRF CFP/YFP FRET cube for 457 laser line

[Sebastian Wasserstrom]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear all
We have a Nikon Ti-E TIRF setup with an Melles griot argon laser. We are having trouble finding suitable FRET filter sets that fit the 457 nm side line, excitation is cut before 457. It is a fairly common laser as I understand it so there should be filters that match. Anyone else had this issue and solved it?
Thanks!

Regards,
Sebastian

Sebastian Wasserstrom, Ph.D.
Assistant researcher
Microscopy platform

LBIC | Medical Faculty | Lund University
Box 118, 221 00 Lund   Besöksadress Klinikgatan 32
Telefon [phone] E-post [hidden email]<mailto:[hidden email]>
Mobiltelefon [phone] Hemsida www.lbic.lu.se<http://www.lbic.lu.se/>

[Periasamy, Ammasi (ap3t)-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hello Sebatian,
If your argon laser is about 40 or 50 mW, you may get enough power at 457 nm for TIRF. Argon laser peak intensity is at 488 nm, you can get enough power at 514 nm too, but, NOT at 457 nm.
We have the Melles griot argon laser (30 mW) with our TIRF. Of course we never used CFP-YFP pair. Currently we are using green diode laser and argon laser 488nm for TIRF FRET (GFP-RFP).
Hope this helps.

Dr. Ammasi Periasamy
Center Director, WM Keck Center for Cellular Imaging,
Prof. of Biology & Biomed. Eng., University of Virginia,
Physical & Life Sciences Building (PLSB 005)
90 Geldard Dr., Charlottesville, VA 22904, USA.

http://www.kcci.virginia.edu/people/profile/ap3t
Phone: [phone] or 982-4869
Fax: [phone]
E-mail: [hidden email]

19th Annual FRET & FLIM Workshop-March 09-13, 2020: http://www.kcci.virginia.edu/workshop 



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Sebastian Wasserstrom
Sent: Tuesday, May 14, 2019 4:01 AM
To: [hidden email]
Subject: TIRF CFP/YFP FRET cube for 457 laser line

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear all
We have a Nikon Ti-E TIRF setup with an Melles griot argon laser. We are having trouble finding suitable FRET filter sets that fit the 457 nm side line, excitation is cut before 457. It is a fairly common laser as I understand it so there should be filters that match. Anyone else had this issue and solved it?
Thanks!

Regards,
Sebastian

Sebastian Wasserstrom, Ph.D.
Assistant researcher
Microscopy platform

LBIC | Medical Faculty | Lund University
Box 118, 221 00 Lund   Besöksadress Klinikgatan 32
Telefon [phone] E-post [hidden email]<mailto:[hidden email]>
Mobiltelefon [phone] Hemsida www.lbic.lu.se<http://www.lbic.lu.se/>

[Sylvie Le Guyader]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Sebastian

Are you talking about an excitation filter? You do not need any since this is a laser. Just buy a cube with dichroic mirror and emission filter.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7C,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website
Follow our microscopy blog!

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sebastian Wasserstrom
Sent: 14 May 2019 10:01
To: [hidden email]
Subject: TIRF CFP/YFP FRET cube for 457 laser line

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear all
We have a Nikon Ti-E TIRF setup with an Melles griot argon laser. We are having trouble finding suitable FRET filter sets that fit the 457 nm side line, excitation is cut before 457. It is a fairly common laser as I understand it so there should be filters that match. Anyone else had this issue and solved it?
Thanks!

Regards,
Sebastian

Sebastian Wasserstrom, Ph.D.
Assistant researcher
Microscopy platform

LBIC | Medical Faculty | Lund University
Box 118, 221 00 Lund   Besöksadress Klinikgatan 32
Telefon [phone] E-post [hidden email]<mailto:[hidden email]>
Mobiltelefon [phone] Hemsida www.lbic.lu.se<http://www.lbic.lu.se/>


När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>.


Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>.

[Jeffrey Carmichael]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

 *COMMERCIAL RESPONSE*

Hi Sebastien,

If you contact me directly, I can help with an appropriate filter set,
mounted appropriately for TIRF.

BTW, yes you do also need an excitation filter. Lasers still need clean-up
filters, whether it's an Ar laser (with multiple lines which must be
completely blocked, not just by AOTF), or especially if any are diode
lasers.

Jeff

*Jeff Carmichael*

*Director of Marketing*

*[hidden email] <[hidden email]> | 802-428-2528*

* <[hidden email]>*


On Tue, May 14, 2019 at 4:34 AM Sebastian Wasserstrom <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all
> We have a Nikon Ti-E TIRF setup with an Melles griot argon laser. We are
> having trouble finding suitable FRET filter sets that fit the 457 nm side
> line, excitation is cut before 457. It is a fairly common laser as I
> understand it so there should be filters that match. Anyone else had this
> issue and solved it?
> Thanks!
>
> Regards,
> Sebastian
>
> Sebastian Wasserstrom, Ph.D.
> Assistant researcher
> Microscopy platform
>
> LBIC | Medical Faculty | Lund University
> Box 118, 221 00 Lund   Besöksadress Klinikgatan 32
> Telefon [phone] E-post [hidden email]<mailto:
> [hidden email]>
> Mobiltelefon [phone] Hemsida www.lbic.lu.se<http://www.lbic.lu.se/
> >
>

--
 <https://www.chroma.com/>CHROMA TECHNOLOGY CORP®
*an employee owned
company*
10 Imtec Lane, Bellows Falls, Vermont 05101 USA
800-824-7662 |
FAX: [phone]
www.chroma.com <https://www.chroma.com/> | 
[hidden email] <mailto:[hidden email]>