*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy*****
Dear all,
I am desperately looking to exchange our currently used tdtomato with a smaller
and more bleach-resistant red protein (2p excitation, in vivo imaging, cortex).
Tsien's TagRFP-t (
http://goo.gl/hTUR2) is the obvious choice - however so far
there is surprisingly little published with this protein. Even though it looks perfect
on paper I am therefore a little reluctant to generate expensive viruses with it.
Does anyone of you have experience concerning performance and especially
toxicity of TagRFP-T - ideally with expression in sensitive mammalian neurons?
Thanks,
Tobias
Locked
