TagRFP

[simon walker (BI)]

Dear List,
We are interested in using TagRFP to create a fusion protein which will
hopefully express in neuronal cells and possibly be used to create a transgenic
mouse.  However, there seems to be very little in the literature describing the
use of this fluorescent protein beyond the original Nature Methods paper.  Has
anyone out there used TagRFP successfully or otherwise?
I'd be interested to hear any experiences positive or negative.
Thanks,
Simon

[Christian-103]

Simon,

What I have found is that "RFP" is being used inconsistently to mean several different constructs from several different  organisms.  Maybe this matters for your cells?  I work nearly all on plants, so I am not even going to guess at your system.

Christian



--- On Fri, 4/30/10, Simon Walker <[hidden email]> wrote:

From: Simon Walker <[hidden email]>
Subject: TagRFP
To: [hidden email]
Date: Friday, April 30, 2010, 9:15 AM

Dear List,
We are interested in using TagRFP to create a fusion protein which will
hopefully express in neuronal cells and possibly be used to create a transgenic
mouse.  However, there seems to be very little in the literature describing the
use of this fluorescent protein beyond the original Nature Methods paper.  Has
anyone out there used TagRFP successfully or otherwise?
I'd be interested to hear any experiences positive or negative.
Thanks,
Simon

[Stephen Bunnell]

Dear Simon,

    I'd advise using TagRFP-Turbo rather than TagRFP, as it is supposed to
have greatly increased photostability. We are just now moving TagRFP-Turbo
into our routine cloning vectors; we could tell you more about it soon. For
now, we can say that it expressed very well in transient transfections, and
was very bright by flow cytometry. We've not yet put it on the scope. Feel
free to contact me in 2-3 weeks if you're still interested.

    Best regards,
 
        -Steve Bunnell


On 4/30/10 10:15 AM, "Simon Walker" <[hidden email]> wrote:

> Dear List,
> We are interested in using TagRFP to create a fusion protein which will
> hopefully express in neuronal cells and possibly be used to create a
> transgenic
> mouse.  However, there seems to be very little in the literature describing
> the
> use of this fluorescent protein beyond the original Nature Methods paper.  Has
> anyone out there used TagRFP successfully or otherwise?
> I'd be interested to hear any experiences positive or negative.
> Thanks,
> Simon

****************************************************************************
Stephen C. Bunnell, Ph.D.
Assistant Professor
Tufts University Medical School
Department of Pathology
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