Temperature and Humidity

[SUBSCRIBE CONFOCALMICROSCOPY Anonymous-3]

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Dear Microscopists,

Is there a standard temperature and humidity setting for an imaging core
suite where confocal and wide-field microscopes are stationed?

Thanks for your time.

Best regards,
Core Imaging Facility Staff
NTU

[Benjamin Smith]

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Shoot for 40% relative humidity and 21.5oC.  Humidity can normally be 20%
to 60% while the temperature you will want to be as stable as possible to
reduce thermal creep in the optics.  Most microscopes have a spec sheet
that states the range they can optimally operate in.

On Thu, Oct 5, 2017 at 8:18 PM, Balakrishnan Kannan <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Microscopists,
>
> Is there a standard temperature and humidity setting for an imaging core
> suite where confocal and wide-field microscopes are stationed?
>
> Thanks for your time.
>
> Best regards,
> Core Imaging Facility Staff
> NTU
>

--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
195 Life Sciences Addition
Berkeley, CA  94720-3200
Tel  [phone]
Fax [phone]
e-mail: [hidden email]
http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>

[SUBSCRIBE CONFOCALMICROSCOPY Anonymous-3]

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Dear Benjamin,

Thanks. Being in a tropical climate we have a high ambient humidity,
typically >70%. Some of the lab areas have the AHU that can maintain the
humidity around 55-60%. If I ask for lower than that it requires portable
dehumidifier. It adds to the power bill. So we want to be optimal so as to
maintain the optics free of mould and at the same time reducing
additional costs.

Temperature can be maintained in the range of 22-23 deg C. I believe that
is fine as long as it does not vary much.

Best regards,
Core Imaging Facility Staff
NTU


On 6 October 2017 at 12:07, Benjamin E Smith <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Shoot for 40% relative humidity and 21.5oC.  Humidity can normally be 20%
> to 60% while the temperature you will want to be as stable as possible to
> reduce thermal creep in the optics.  Most microscopes have a spec sheet
> that states the range they can optimally operate in.
>
> On Thu, Oct 5, 2017 at 8:18 PM, Balakrishnan Kannan <
> [hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear Microscopists,
> >
> > Is there a standard temperature and humidity setting for an imaging core
> > suite where confocal and wide-field microscopes are stationed?
> >
> > Thanks for your time.
> >
> > Best regards,
> > Core Imaging Facility Staff
> > NTU
> >
>
>
>
> --
> Benjamin E. Smith, Ph. D.
> Imaging Specialist, Vision Science
> University of California, Berkeley
> 195 Life Sciences Addition
> Berkeley, CA  94720-3200
> Tel  [phone]
> Fax [phone]
> e-mail: [hidden email]
> http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>
>

[Benjamin Smith]

*****
To join, leave or search the confocal microscopy listserv, go to:
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Post images on http://www.imgur.com and include the link in your posting.
*****

Right, with optics a stable temperature is more important than an absolute
temperature, as long as the temperature is around room temp.  For humidity,
the biggest concern is condensation damaging the optics.  Basically, the
concern is that say the cooling had a hard time and the room is at 26oC and
70% humidity.  If the temperature then drops to 20oC during an AC cycle,
the relative humidity will then be approaching 100% inside the scope, which
will definitely damage antireflective coatings and dichroic mirrors.  That
is why spec sheets say 60% humidity max, as this give plenty of safety
buffer for temperature fluctuations without risking condensation.  A data
logger is invaluable, both for your own records, and any insurance claims
if necessary.  Also, past 60% humidity you will also notice that near-IR
mode-locked lasers don't perform as well.

-Ben Smith

On Thu, Oct 5, 2017 at 10:15 PM, Balakrishnan Kannan <[hidden email]
> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Benjamin,
>
> Thanks. Being in a tropical climate we have a high ambient humidity,
> typically >70%. Some of the lab areas have the AHU that can maintain the
> humidity around 55-60%. If I ask for lower than that it requires portable
> dehumidifier. It adds to the power bill. So we want to be optimal so as to
> maintain the optics free of mould and at the same time reducing
> additional costs.
>
> Temperature can be maintained in the range of 22-23 deg C. I believe that
> is fine as long as it does not vary much.
>
> Best regards,
> Core Imaging Facility Staff
> NTU
>
>
> On 6 October 2017 at 12:07, Benjamin E Smith <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Shoot for 40% relative humidity and 21.5oC.  Humidity can normally be 20%
> > to 60% while the temperature you will want to be as stable as possible to
> > reduce thermal creep in the optics.  Most microscopes have a spec sheet
> > that states the range they can optimally operate in.
> >
> > On Thu, Oct 5, 2017 at 8:18 PM, Balakrishnan Kannan <
> > [hidden email]>
> > wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > Post images on http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Dear Microscopists,
> > >
> > > Is there a standard temperature and humidity setting for an imaging
> core
> > > suite where confocal and wide-field microscopes are stationed?
> > >
> > > Thanks for your time.
> > >
> > > Best regards,
> > > Core Imaging Facility Staff
> > > NTU
> > >
> >
> >
> >
> > --
> > Benjamin E. Smith, Ph. D.
> > Imaging Specialist, Vision Science
> > University of California, Berkeley
> > 195 Life Sciences Addition
> > Berkeley, CA  94720-3200
> > Tel  [phone]
> > Fax [phone]
> > e-mail: [hidden email]
> > http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>
> >
>

--
Benjamin E. Smith, Ph. D.
Imaging Specialist, Vision Science
University of California, Berkeley
195 Life Sciences Addition
Berkeley, CA  94720-3200
Tel  [phone]
Fax [phone]
e-mail: [hidden email]
http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>

[SUBSCRIBE CONFOCALMICROSCOPY Anonymous-3]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks Ben for the insight.

That brings me to another question: We have a confocal and a lightsheet in
a room within the fish facility. Now the fish facility requires 26 degC for
the fish. Will it (26 deg C and humidity < 60% and both maintained quite
well) affect?

On 6 October 2017 at 13:53, Benjamin E Smith <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Right, with optics a stable temperature is more important than an absolute
> temperature, as long as the temperature is around room temp.  For humidity,
> the biggest concern is condensation damaging the optics.  Basically, the
> concern is that say the cooling had a hard time and the room is at 26oC and
> 70% humidity.  If the temperature then drops to 20oC during an AC cycle,
> the relative humidity will then be approaching 100% inside the scope, which
> will definitely damage antireflective coatings and dichroic mirrors.  That
> is why spec sheets say 60% humidity max, as this give plenty of safety
> buffer for temperature fluctuations without risking condensation.  A data
> logger is invaluable, both for your own records, and any insurance claims
> if necessary.  Also, past 60% humidity you will also notice that near-IR
> mode-locked lasers don't perform as well.
>
> -Ben Smith
>
> On Thu, Oct 5, 2017 at 10:15 PM, Balakrishnan Kannan <
> [hidden email]
> > wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear Benjamin,
> >
> > Thanks. Being in a tropical climate we have a high ambient humidity,
> > typically >70%. Some of the lab areas have the AHU that can maintain the
> > humidity around 55-60%. If I ask for lower than that it requires portable
> > dehumidifier. It adds to the power bill. So we want to be optimal so as
> to
> > maintain the optics free of mould and at the same time reducing
> > additional costs.
> >
> > Temperature can be maintained in the range of 22-23 deg C. I believe that
> > is fine as long as it does not vary much.
> >
> > Best regards,
> > Core Imaging Facility Staff
> > NTU
> >
> >
> > On 6 October 2017 at 12:07, Benjamin E Smith <
> [hidden email]>
> > wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > Post images on http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Shoot for 40% relative humidity and 21.5oC.  Humidity can normally be
> 20%
> > > to 60% while the temperature you will want to be as stable as possible
> to
> > > reduce thermal creep in the optics.  Most microscopes have a spec sheet
> > > that states the range they can optimally operate in.
> > >
> > > On Thu, Oct 5, 2017 at 8:18 PM, Balakrishnan Kannan <
> > > [hidden email]>
> > > wrote:
> > >
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > > Post images on http://www.imgur.com and include the link in your
> > > posting.
> > > > *****
> > > >
> > > > Dear Microscopists,
> > > >
> > > > Is there a standard temperature and humidity setting for an imaging
> > core
> > > > suite where confocal and wide-field microscopes are stationed?
> > > >
> > > > Thanks for your time.
> > > >
> > > > Best regards,
> > > > Core Imaging Facility Staff
> > > > NTU
> > > >
> > >
> > >
> > >
> > > --
> > > Benjamin E. Smith, Ph. D.
> > > Imaging Specialist, Vision Science
> > > University of California, Berkeley
> > > 195 Life Sciences Addition
> > > Berkeley, CA  94720-3200
> > > Tel  [phone]
> > > Fax [phone]
> > > e-mail: [hidden email]
> > > http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>
> > >
> >
>
>
>
> --
> Benjamin E. Smith, Ph. D.
> Imaging Specialist, Vision Science
> University of California, Berkeley
> 195 Life Sciences Addition
> Berkeley, CA  94720-3200
> Tel  [phone]
> Fax [phone]
> e-mail: [hidden email]
> http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>
>

[Craig Brideau]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

As you raise temperature you need more water vapor in the air to maintain
%RH, so it should actually be easier to hold a lower RH at a higher
temperature.
From personal experience, I recommend you invest one or two accurate
dataloggers that measure temperature and humidity and use them to monitor
the room. In the contract for installation of controls, clearly state the
specifications the system is required to meet. The dataloggers will provide
the evidence for the system performing (or not) as required in the
installation contract. I have used MSR dataloggers in the past (no
commercial interest, just a user of the product) but many vendors sell
equivalent loggers.

Craig

On Fri, Oct 6, 2017 at 12:06 AM, Balakrishnan Kannan <[hidden email]
> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Thanks Ben for the insight.
>
> That brings me to another question: We have a confocal and a lightsheet in
> a room within the fish facility. Now the fish facility requires 26 degC for
> the fish. Will it (26 deg C and humidity < 60% and both maintained quite
> well) affect?
>
> On 6 October 2017 at 13:53, Benjamin E Smith <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Right, with optics a stable temperature is more important than an
> absolute
> > temperature, as long as the temperature is around room temp.  For
> humidity,
> > the biggest concern is condensation damaging the optics.  Basically, the
> > concern is that say the cooling had a hard time and the room is at 26oC
> and
> > 70% humidity.  If the temperature then drops to 20oC during an AC cycle,
> > the relative humidity will then be approaching 100% inside the scope,
> which
> > will definitely damage antireflective coatings and dichroic mirrors.
> That
> > is why spec sheets say 60% humidity max, as this give plenty of safety
> > buffer for temperature fluctuations without risking condensation.  A data
> > logger is invaluable, both for your own records, and any insurance claims
> > if necessary.  Also, past 60% humidity you will also notice that near-IR
> > mode-locked lasers don't perform as well.
> >
> > -Ben Smith
> >
> > On Thu, Oct 5, 2017 at 10:15 PM, Balakrishnan Kannan <
> > [hidden email]
> > > wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > Post images on http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Dear Benjamin,
> > >
> > > Thanks. Being in a tropical climate we have a high ambient humidity,
> > > typically >70%. Some of the lab areas have the AHU that can maintain
> the
> > > humidity around 55-60%. If I ask for lower than that it requires
> portable
> > > dehumidifier. It adds to the power bill. So we want to be optimal so as
> > to
> > > maintain the optics free of mould and at the same time reducing
> > > additional costs.
> > >
> > > Temperature can be maintained in the range of 22-23 deg C. I believe
> that
> > > is fine as long as it does not vary much.
> > >
> > > Best regards,
> > > Core Imaging Facility Staff
> > > NTU
> > >
> > >
> > > On 6 October 2017 at 12:07, Benjamin E Smith <
> > [hidden email]>
> > > wrote:
> > >
> > > > *****
> > > > To join, leave or search the confocal microscopy listserv, go to:
> > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > > Post images on http://www.imgur.com and include the link in your
> > > posting.
> > > > *****
> > > >
> > > > Shoot for 40% relative humidity and 21.5oC.  Humidity can normally be
> > 20%
> > > > to 60% while the temperature you will want to be as stable as
> possible
> > to
> > > > reduce thermal creep in the optics.  Most microscopes have a spec
> sheet
> > > > that states the range they can optimally operate in.
> > > >
> > > > On Thu, Oct 5, 2017 at 8:18 PM, Balakrishnan Kannan <
> > > > [hidden email]>
> > > > wrote:
> > > >
> > > > > *****
> > > > > To join, leave or search the confocal microscopy listserv, go to:
> > > > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > > > > Post images on http://www.imgur.com and include the link in your
> > > > posting.
> > > > > *****
> > > > >
> > > > > Dear Microscopists,
> > > > >
> > > > > Is there a standard temperature and humidity setting for an imaging
> > > core
> > > > > suite where confocal and wide-field microscopes are stationed?
> > > > >
> > > > > Thanks for your time.
> > > > >
> > > > > Best regards,
> > > > > Core Imaging Facility Staff
> > > > > NTU
> > > > >
> > > >
> > > >
> > > >
> > > > --
> > > > Benjamin E. Smith, Ph. D.
> > > > Imaging Specialist, Vision Science
> > > > University of California, Berkeley
> > > > 195 Life Sciences Addition
> > > > Berkeley, CA  94720-3200
> > > > Tel  [phone]
> > > > Fax [phone]
> > > > e-mail: [hidden email]
> > > > http://vision.berkeley.edu/?page_id=5635 <
> http://vision.berkeley.edu/>
> > > >
> > >
> >
> >
> >
> > --
> > Benjamin E. Smith, Ph. D.
> > Imaging Specialist, Vision Science
> > University of California, Berkeley
> > 195 Life Sciences Addition
> > Berkeley, CA  94720-3200
> > Tel  [phone]
> > Fax [phone]
> > e-mail: [hidden email]
> > http://vision.berkeley.edu/?page_id=5635 <http://vision.berkeley.edu/>
> >
>