Temperature controler for upright microscopes

[Jean-Yves Tinevez-3]

Dear friends of imaging,

I have been running in temperature problems, trying to image C.elegans at
20-22ºC, in a very warm microscopy room. At best, the objective is at 25ºC,
and that seriously compromises my imaging condition. I have been looking for
a temperature controller that would allow to *cool* down a slide to the 20ºC
range, and was wondering if you had any experience with such a thing.

I typically have a Zeiss stage, and use classical microscope glass slides. I
was directed to the Harvard Apparatus microincubation chamber:

http://www.harvardapparatus.com/webapp/wcs/stores/servlet/product_11051_10001_39704_-1_HAI_ProductDetail___

It works on slides, but this is a bit overkill, and does not work on upright
microscopes (only inverted).

I was wondering if you had a product in mind that allow the close
temperature control of slides, and that would fit on an upright microscope.

Any help would be greatly appreciated.
Best
jyt

[Beat Ludin]

[Commercial interest]

Dear Jean-Yves

Our iceCUBE&BOX system allows you to work at
temperatures down to 10°C below ambient and can
be supplied for any microscope. Because the
entire microscope is cooled down, you can use it
with any stage and sample format and there's no
need for a cumbersome objective cooler to avoid a
heat bridge in the area you are imaging. If you
are interested, please contact me off-list for further information.

Best regards,  Beat

At 16:15 08-03-10, you wrote:

>Dear friends of imaging,
>
>I have been running in temperature problems, trying to image C.elegans at
>20-22ºC, in a very warm microscopy room. At best, the objective is at 25ºC,
>and that seriously compromises my imaging condition. I have been looking for
>a temperature controller that would allow to *cool* down a slide to the 20ºC
>range, and was wondering if you had any experience with such a thing.
>
>I typically have a Zeiss stage, and use classical microscope glass slides. I
>was directed to the Harvard Apparatus microincubation chamber:
>
>http://www.harvardapparatus.com/webapp/wcs/stores/servlet/product_11051_10001_39704_-1_HAI_ProductDetail___
>
>It works on slides, but this is a bit overkill, and does not work on upright
>microscopes (only inverted).
>
>I was wondering if you had a product in mind that allow the close
>temperature control of slides, and that would fit on an upright microscope.
>
>Any help would be greatly appreciated.
>Best
>jyt

[Dale Callaham]

There is a very good section on the Molecular Expressions website for
live cell imaging and culture chambers.
http://micro.magnet.fsu.edu/primer/techniques/livecellimaging/index.html

The link for culture chambers takes you to the Nikon MicroscopyU website
and they discuss many types of chambers.
http://www.microscopyu.com/articles/livecellimaging/culturechambers.html

Nothing is going to be simple, but a closed chamber with circulated
cooled culture medium is an option. A blower with a Peltier cooled air
stream will cool the whole stage environment including the objective.
You are very close to ambient, but you still may need to dehumidify the
room to keep from getting condensation on things.

Dale


Jean-Yves Tinevez wrote:

> Dear friends of imaging,
>
> I have been running in temperature problems, trying to image C.elegans at
> 20-22ºC, in a very warm microscopy room. At best, the objective is at 25ºC,
> and that seriously compromises my imaging condition. I have been looking for
> a temperature controller that would allow to *cool* down a slide to the 20ºC
> range, and was wondering if you had any experience with such a thing.
>
> I typically have a Zeiss stage, and use classical microscope glass slides. I
> was directed to the Harvard Apparatus microincubation chamber:
>
> http://www.harvardapparatus.com/webapp/wcs/stores/servlet/product_11051_10001_39704_-1_HAI_ProductDetail___
>
> It works on slides, but this is a bit overkill, and does not work on upright
> microscopes (only inverted).
>
> I was wondering if you had a product in mind that allow the close
> temperature control of slides, and that would fit on an upright microscope.
>
> Any help would be greatly appreciated.
> Best
> jyt

[mahogny]

On Mon, 2010-03-08 at 09:15 -0600, Jean-Yves Tinevez wrote:

> Dear friends of imaging,
>
> I have been running in temperature problems, trying to image C.elegans at
> 20-22ºC, in a very warm microscopy room. At best, the objective is at 25ºC,
> and that seriously compromises my imaging condition. I have been looking for
> a temperature controller that would allow to *cool* down a slide to the 20ºC
> range, and was wondering if you had any experience with such a thing.
>
> I typically have a Zeiss stage, and use classical microscope glass slides. I
> was directed to the Harvard Apparatus microincubation chamber:
>
> http://www.harvardapparatus.com/webapp/wcs/stores/servlet/product_11051_10001_39704_-1_HAI_ProductDetail___
>
> It works on slides, but this is a bit overkill, and does not work on upright
> microscopes (only inverted).
>
> I was wondering if you had a product in mind that allow the close
> temperature control of slides, and that would fit on an upright microscope.
>
> Any help would be greatly appreciated.
> Best
> jyt

we also image c.elegans and had some temperature problems but those got
resolved once we kicked out our halogen lamps and designed our own LED
system (one LED driven by a konrad microcontroller).

can't speak much about temperature control otherwise (we just monitor
it, with the same konrad device) but if you're handy then you can build
some rather fancy stuff yourself very cheaply. a sensor that you put
near the slide, peltier elements for cooling, some plexiglass, the
controller, and you are done.

in your case, maybe you even can attach the sensor and peltier on the
slide with CPU paste

/Johan

[Mark Auty]

I have several Linkam microscope stages that I use with upright microscopes. I use this Peltier PE120 stage one for cooling from ambient down to ~-10oC:

http://www.linkam.co.uk/index.php?option=com_virtuemart&page=shop.browse&category_id=12&Itemid=70


I just use normal microscope slides resting on the Peltier-cooled surface. Condensation could be a problem and usually requires piped cooled air/nitrogen gas. However, using a small fan close to the objective might be OK, since you are only cooling from 25 to 20C.


Regards
Mark



Dr Mark Auty
Manager, National Food Imaging Centre
Food Processing & Functionality Department
Moorepark Food Research Centre
Teagasc Moorepark
Fermoy, Co Cork
Ireland
tel: [phone]
fax: [phone]
[hidden email]



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jean-Yves Tinevez
Sent: 08 March 2010 15:15
To: [hidden email]
Subject: Temperature controler for upright microscopes

Dear friends of imaging,

I have been running in temperature problems, trying to image C.elegans at
20-22ºC, in a very warm microscopy room. At best, the objective is at 25ºC,
and that seriously compromises my imaging condition. I have been looking for
a temperature controller that would allow to *cool* down a slide to the 20ºC
range, and was wondering if you had any experience with such a thing.

I typically have a Zeiss stage, and use classical microscope glass slides. I
was directed to the Harvard Apparatus microincubation chamber:

http://www.harvardapparatus.com/webapp/wcs/stores/servlet/product_11051_10001_39704_-1_HAI_ProductDetail___

It works on slides, but this is a bit overkill, and does not work on upright
microscopes (only inverted).

I was wondering if you had a product in mind that allow the close
temperature control of slides, and that would fit on an upright microscope.

Any help would be greatly appreciated.
Best
jyt
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[Louis Kerr]

We have a stage system on our Zeiss LSM 510 inverted microscope that can cool and heat based on a Pecon stage insert http://www.pecon.biz/?page_id=314  and a Thermo NESLAB RTE refrigerated bath/circulator with water and ethylene glycol. We have operated it between 2-40C. I would think you could find a plate for an upright or I have seen home built models of aluminum plate with tubes attached or embedded.  Because you are so close to ambient temperatures you would be best off with a device that cools and heats.

Louie


----- Original Message -----
From: "Dale Callaham" <[e-mail]>
To: [e-mail]
Sent: Monday, March 8, 2010 10:40:02 AM
Subject: Re: Temperature controler for upright microscopes

There is a very good section on the Molecular Expressions website for
live cell imaging and culture chambers.
http://micro.magnet.fsu.edu/primer/techniques/livecellimaging/index.html

The link for culture chambers takes you to the Nikon MicroscopyU website
and they discuss many types of chambers.
http://www.microscopyu.com/articles/livecellimaging/culturechambers.html

Nothing is going to be simple, but a closed chamber with circulated
cooled culture medium is an option. A blower with a Peltier cooled air
stream will cool the whole stage environment including the objective.
You are very close to ambient, but you still may need to dehumidify the
room to keep from getting condensation on things.

Dale


Jean-Yves Tinevez wrote:

> Dear friends of imaging,
>
> I have been running in temperature problems, trying to image C.elegans at
> 20-22ºC, in a very warm microscopy room. At best, the objective is at 25ºC,
> and that seriously compromises my imaging condition. I have been looking for
> a temperature controller that would allow to *cool* down a slide to the 20ºC
> range, and was wondering if you had any experience with such a thing.
>
> I typically have a Zeiss stage, and use classical microscope glass slides. I
> was directed to the Harvard Apparatus microincubation chamber:
>
> http://www.harvardapparatus.com/webapp/wcs/stores/servlet/product_11051_10001_39704_-1_HAI_ProductDetail___
>
> It works on slides, but this is a bit overkill, and does not work on upright
> microscopes (only inverted).
>
> I was wondering if you had a product in mind that allow the close
> temperature control of slides, and that would fit on an upright microscope.
>
> Any help would be greatly appreciated.
> Best
> jyt

--

Louis Kerr
[e-mail]

Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA  02543
508-289-7273
508-540-6902 (FAX)
508-292-0289 (Cell phone)

VISIT OUR WEB SITES:
www.mbl.edu
www.courses.mbl.edu

[Dan Focht]

Jean-Yves


Bioptechs develops and manufacturers a unique line of micro-environmental control systems for time lapse imaging.
We pay very close attention to your exact needs.  There are many approaches to micro-environmental control.
To make the selection process easier and make sure we cover all the factors to ensure a successfully working system,
 please fill out our User Profile Questionnaire available from home page. www.bioptechs.com

Temperature control on an upright scope, close to ambient is a little more tricky but we can do it and eliminate condensation at the same time.


Dan


On Mar 8, 2010, at 10:15 AM, Jean-Yves Tinevez wrote:

Dear friends of imaging,

I have been running in temperature problems, trying to image C.elegans at
20-22ºC, in a very warm microscopy room. At best, the objective is at 25ºC,
and that seriously compromises my imaging condition. I have been looking for
a temperature controller that would allow to *cool* down a slide to the 20ºC
range, and was wondering if you had any experience with such a thing.

I typically have a Zeiss stage, and use classical microscope glass slides. I
was directed to the Harvard Apparatus microincubation chamber:

http://www.harvardapparatus.com/webapp/wcs/stores/servlet/product_11051_10001_39704_-1_HAI_ProductDetail___

It works on slides, but this is a bit overkill, and does not work on upright
microscopes (only inverted).

I was wondering if you had a product in mind that allow the close
temperature control of slides, and that would fit on an upright microscope.

Any help would be greatly appreciated.
Best
jyt

Dan Focht
Bioptechs Inc
P: [phone]
F: [phone]
[hidden email]

[Andreas Bruckbauer]

Hello,
we recently had some trouble caused by the curtain in our microscope room, it builds up electric charge under dry weather conditions leading to small electric shocks when users touch the optical table. Has anyone encountered such a problem and found a solution? Are there any antistatic black curtains which would do better?

Andreas

[EricMarino]

We’ve purchased curtains from a local hospital supply distributer, since they deal with static sensitive medical devices they’re antistatic, however, you will not get them in black so we tested white versus black curtains and saw no difference in ambient light leaking into the scopes.

 

 

Eric Marino

[hidden email]

 

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Andreas Bruckbauer
Sent: Monday, March 08, 2010 11:34 AM
To: [hidden email]
Subject: antistatic curtains for microscopy room

 

Hello,
we recently had some trouble caused by the curtain in our microscope room, it builds up electric charge under dry weather conditions leading to small electric shocks when users touch the optical table. Has anyone encountered such a problem and found a solution? Are there any antistatic black curtains which would do better?

Andreas

 

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[S. Brunet]

Hello:
We have a static dissipating floor.  This was done when the lab was built (I
assume it is grounded).  The drawback is that most shoes soles are good
insulators.  Therefore the use of shoe grounding strap is necessary.
Sophie
____________________________________________________
Sophie M. K. Brunet, Ph. D.
Research Officer
Optical Spectroscopy, Laser Systems and Applications
[hidden email]
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Quoting Andreas Bruckbauer <[hidden email]>:

>
>  Hello,
> we recently had some trouble caused by the curtain in our microscope room, it
> builds up electric charge under dry weather conditions leading to small
> electric shocks when users touch the optical table. Has anyone encountered
> such a problem and found a solution? Are there any antistatic black curtains
> which would do better?
>
> Andreas
>
>
>
>
>

[Jean-Yves Tinevez-3]

Dear friends of imaging,

Thank you all for the fantastic input!
I will carefully look into all of it.
Thanks again
Best
jy


--
Jean-Yves Tinevez
PFID - Imagopole
Institut Pasteur
25-28, rue du Docteur Roux
75724 Paris cedex 15
France
tel: [phone]