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Temperature logger for microscope enclosure

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Seamus Holden-2

Temperature logger for microscope enclosure

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Dear colleagues

I am looking for a fairly high sensitivity thermometer with probe to monitor the temperature of the microscope enclosure for our SIM microscope. It should be decent precision, +/-0.05C, so my understanding is that a thermocouple based sensor won't cut it. Ideally it should have some logging capability. Does anyone have any recommendations of suitable equipment?

Many thanks,
Seamus

Dr Seamus Holden
University Research Fellow

Centre for Bacterial Cell Biology
Baddiley-Clark Building
Newcastle University
Richardson Road
Newcastle upon Tyne
NE2 4AX, United Kingdom

Phone: +44 (0)191 208 3230

Julio MATEOS_LANGERAK

Re: Temperature logger for microscope enclosure

Hi,

We use across the whole facility an openHW solution provided (and also commercialised) at www.openenergymonitor.org. Probes (emonTH’s) are distributed everywhere (including our 3D-SIM enclosure) and are sensing temperature at regular intervals. The data is sent through radio signals to a base station (emonBase) on a Raspberry PI. This one collects and logs all the data into feeds and generates a configurable website where you may visualise everything. Pretty neat I have to say and very cheap compared to other more ‘commercial’ solutions.

Best, Julio

__________________________________________
Julio Mateos Langerak, PhD.
OMX manager
Arnaud de Villeneuve Campus Imaging Facility
Montpellier RIO Imaging
Montpellier BIOCAMPUS, UMS3426
Institut de Génétique Humaine-CNRS
141, rue de la Cardonille
F-34396 Montpellier (France)
e-mail: [hidden email]
phone: +33.4.34.35.99.90
fax: +33.4.34.35.99.01
URL: http://www.mri.cnrs.fr/
__________________________________________

> On 28 Apr 2016, at 28 Apr-19:01, Seamus Holden <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear colleagues
>
> I am looking for a fairly high sensitivity thermometer with probe to monitor the temperature of the microscope enclosure for our SIM microscope. It should be decent precision, +/-0.05C, so my understanding is that a thermocouple based sensor won't cut it. Ideally it should have some logging capability. Does anyone have any recommendations of suitable equipment?
>
> Many thanks,
> Seamus
>
> Dr Seamus Holden
> University Research Fellow
>
> Centre for Bacterial Cell Biology
> Baddiley-Clark Building
> Newcastle University
> Richardson Road
> Newcastle upon Tyne
> NE2 4AX, United Kingdom
>
> Phone: +44 (0)191 208 3230

Pedro Almada

Re: Temperature logger for microscope enclosure

In reply to this post by Seamus Holden-2

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*****

Hi Seamus,

I don't have experience myself with them but I know some people have used
something like these:
http://www.geminidataloggers.com/data-loggers/tinytag-talk-2/tk-4014

Cheers,
Pedro
On 28 Apr 2016 19:22, "Seamus Holden" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear colleagues
>
> I am looking for a fairly high sensitivity thermometer with probe to
> monitor the temperature of the microscope enclosure for our SIM microscope.
> It should be decent precision, +/-0.05C, so my understanding is that a
> thermocouple based sensor won't cut it. Ideally it should have some logging
> capability. Does anyone have any recommendations of suitable equipment?
>
> Many thanks,
> Seamus
>
> Dr Seamus Holden
> University Research Fellow
>
> Centre for Bacterial Cell Biology
> Baddiley-Clark Building
> Newcastle University
> Richardson Road
> Newcastle upon Tyne
> NE2 4AX, United Kingdom
>
> Phone: +44 (0)191 208 3230
>

Xuejun Sun

Re: Temperature logger for microscope enclosure

In reply to this post by Seamus Holden-2

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Hi,

I am very happy with this:

http://www.onsetcomp.com/products/data-loggers/u12-012

It logs temperature, humidity and light level and it is small enough to be
placed pretty much anywhere. You can set the log intervals.

No commercial interests in the company.

Xuejun

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Seamus Holden
Sent: Thursday, April 28, 2016 11:01 AM
To: [hidden email]
Subject: Temperature logger for microscope enclosure

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear colleagues

I am looking for a fairly high sensitivity thermometer with probe to monitor
the temperature of the microscope enclosure for our SIM microscope. It
should be decent precision, +/-0.05C, so my understanding is that a
thermocouple based sensor won't cut it. Ideally it should have some logging
capability. Does anyone have any recommendations of suitable equipment?

Many thanks,
Seamus

Dr Seamus Holden
University Research Fellow

Centre for Bacterial Cell Biology
Baddiley-Clark Building
Newcastle University
Richardson Road
Newcastle upon Tyne
NE2 4AX, United Kingdom

Phone: +44 (0)191 208 3230

Mark Scott

Re: Temperature logger for microscope enclosure

In reply to this post by Seamus Holden-2

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Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Seamus,

There are some pretty high resolution thermocouple devices out there these days. There is a version from Picotech that will go down to 0.025C according to its specs but I haven't had a chance to test this one out (https://www.picotech.com/data-logger/tc-08/thermocouple-data-logger)

There are also some cheaper USB loggers I have used in the past but these won't have the resolution you are after.

Hope that helps

Mark Scott
Specialist for Intra-vital and Live Cell Imaging
Centre for Dynamic Imaging
The Walter & Eliza Hall Institute
1G Royal Parade, Parkville
Victoria 3052, Australia

----- Original Message -----
From: "Seamus Holden" <[hidden email]>
To: [hidden email]
Sent: Friday, 29 April, 2016 3:01:04 AM
Subject: Temperature logger for microscope enclosure

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear colleagues

I am looking for a fairly high sensitivity thermometer with probe to monitor the temperature of the microscope enclosure for our SIM microscope. It should be decent precision, +/-0.05C, so my understanding is that a thermocouple based sensor won't cut it. Ideally it should have some logging capability. Does anyone have any recommendations of suitable equipment?

Many thanks,
Seamus

Dr Seamus Holden
University Research Fellow

Centre for Bacterial Cell Biology
Baddiley-Clark Building
Newcastle University
Richardson Road
Newcastle upon Tyne
NE2 4AX, United Kingdom

Phone: +44 (0)191 208 3230
______________________________________________________________________

The information in this email is confidential and intended solely for the addressee.
You must not disclose, forward, print or use it without the permission of the sender.
______________________________________________________________________

Andreas Bruckbauer

Re: Temperature logger for microscope enclosure

In reply to this post by Julio MATEOS_LANGERAK

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*****

Hi,

The openHW solution looks great, thanks for sharing it! The only problem is that the original post asked for an accuracy of +/- 0.05C but as i can see the sensors are based on a DS18B20 which has a +/- 0.5C accuracy. I think it is expensive to measure temperature that accurate, you would need to use a resistance temperature sensor like a PT100 and a high impedence voltmeter (this is the expensive bit). It might not be necessary for the proposed application, according to my experience the temperature in the microscope enclosure of our SIM system typically varies by more than 0.05C and the openHW solution should be sufficient.You can also directly connect the raspberry pi to the DS18B20 and log the data. The new model 3 even has on board Wifi and there are touchscreen displays

best wishes

Andreas

-----Original Message-----
From: Julio MATEOS_LANGERAK <[hidden email]>
To: CONFOCALMICROSCOPY <[hidden email]>
Sent: Fri, 29 Apr 2016 9:37
Subject: Re: Temperature logger for microscope enclosure

Hi,

We use across the whole facility an openHW solution provided (and also commercialised) at www.openenergymonitor.org. Probes (emonTH’s) are distributed everywhere (including our 3D-SIM enclosure) and are sensing temperature at regular intervals. The data is sent through radio signals to a base station (emonBase) on a Raspberry PI. This one collects and logs all the data into feeds and generates a configurable website where you may visualise everything. Pretty neat I have to say and very cheap compared to other more ‘commercial’ solutions.

Best, Julio

__________________________________________
Julio Mateos Langerak, PhD.
OMX manager
Arnaud de Villeneuve Campus Imaging Facility
Montpellier RIO Imaging
Montpellier BIOCAMPUS, UMS3426
Institut de Génétique Humaine-CNRS
141, rue de la Cardonille
F-34396 Montpellier (France)
e-mail: [hidden email]
phone: +33.4.34.35.99.90
fax: +33.4.34.35.99.01
URL: http://www.mri.cnrs.fr/
__________________________________________

Andreas Bruckbauer

Re: Temperature logger for microscope enclosure

In reply to this post by Julio MATEOS_LANGERAK

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

This might do the job with 0.015C accuracy (no commercial interest)
https://www.picotech.com/data-logger/pt-104/high-accuracy-temperature-daq

best wishes

Andreas

-----Original Message-----
From: Julio MATEOS_LANGERAK <[hidden email]>
To: CONFOCALMICROSCOPY <[hidden email]>
Sent: Fri, 29 Apr 2016 9:37
Subject: Re: Temperature logger for microscope enclosure

Hi,

We use across the whole facility an openHW solution provided (and also commercialised) at www.openenergymonitor.org. Probes (emonTH’s) are distributed everywhere (including our 3D-SIM enclosure) and are sensing temperature at regular intervals. The data is sent through radio signals to a base station (emonBase) on a Raspberry PI. This one collects and logs all the data into feeds and generates a configurable website where you may visualise everything. Pretty neat I have to say and very cheap compared to other more ‘commercial’ solutions.

Best, Julio

__________________________________________
Julio Mateos Langerak, PhD.
OMX manager
Arnaud de Villeneuve Campus Imaging Facility
Montpellier RIO Imaging
Montpellier BIOCAMPUS, UMS3426
Institut de Génétique Humaine-CNRS
141, rue de la Cardonille
F-34396 Montpellier (France)
e-mail: [hidden email]
phone: +33.4.34.35.99.90
fax: +33.4.34.35.99.01
URL: http://www.mri.cnrs.fr/
__________________________________________

Smith, Benjamin E.

Re: Temperature logger for microscope enclosure

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

One other thing to keep in mind, if you are trying to keep the sample itself within 0.05oC, then a temperature controlled enclosure may be insufficient, as the temperature of the enclosure may not correlate with the temperature of the sample with such precision. If you need to keep a sample at extremely precise temperatures, one thing I'd recommend would be a Peltier stage. We use these both for keeping samples at exactly 37.0oC as well as exactly 22.0oC. For long live-imaging runs, the microscope stage can slightly heat up due to thermal conduction from the electronics. The Peltier stage can offset this both by removing heat and adding heat as needed. A well tuned PID controller will keep the sample temperature within the exacting parameters you described. The stage we've used is the Physitemp TS-4SMP, which can both regulate the temperature of 35mm dishes as well as slides (for microfluidics, etc.): http://www.physitemp.com/products/HeatingandCooling4Scopes/

Hope this helps,
Ben Smith

________________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Andreas Bruckbauer <[hidden email]>
Sent: Friday, April 29, 2016 4:29:52 AM
To: [hidden email]
Subject: Re: Temperature logger for microscope enclosure

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

This might do the job with 0.015C accuracy (no commercial interest)
https://www.picotech.com/data-logger/pt-104/high-accuracy-temperature-daq

best wishes

Andreas

-----Original Message-----
From: Julio MATEOS_LANGERAK <[hidden email]>
To: CONFOCALMICROSCOPY <[hidden email]>
Sent: Fri, 29 Apr 2016 9:37
Subject: Re: Temperature logger for microscope enclosure

Hi,

We use across the whole facility an openHW solution provided (and also commercialised) at www.openenergymonitor.org. Probes (emonTH’s) are distributed everywhere (including our 3D-SIM enclosure) and are sensing temperature at regular intervals. The data is sent through radio signals to a base station (emonBase) on a Raspberry PI. This one collects and logs all the data into feeds and generates a configurable website where you may visualise everything. Pretty neat I have to say and very cheap compared to other more ‘commercial’ solutions.

Best, Julio

__________________________________________
Julio Mateos Langerak, PhD.
OMX manager
Arnaud de Villeneuve Campus Imaging Facility
Montpellier RIO Imaging
Montpellier BIOCAMPUS, UMS3426
Institut de Génétique Humaine-CNRS
141, rue de la Cardonille
F-34396 Montpellier (France)
e-mail: [hidden email]
phone: +33.4.34.35.99.90
fax: +33.4.34.35.99.01
URL: http://www.mri.cnrs.fr/
__________________________________________

Craig Brideau

Re: Temperature logger for microscope enclosure

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

I've used a few MSR dataloggers for monitoring temperature and humidity.
Many versions are available and some support analog in so you can attach
and monitor your own arbitrary sensors. An optical power meter output is an
obvious thing to add to your temperature and humidity measurements. Some
power meters have an analog out that you can patch directly into the
datalogger for recording simultaneously with the other parameters you want
to watch.

Craig

On Fri, Apr 29, 2016 at 10:01 AM, Smith, Benjamin E. <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> One other thing to keep in mind, if you are trying to keep the sample
> itself within 0.05oC, then a temperature controlled enclosure may be
> insufficient, as the temperature of the enclosure may not correlate with
> the temperature of the sample with such precision. If you need to keep a
> sample at extremely precise temperatures, one thing I'd recommend would be
> a Peltier stage. We use these both for keeping samples at exactly 37.0oC
> as well as exactly 22.0oC. For long live-imaging runs, the microscope
> stage can slightly heat up due to thermal conduction from the electronics.
> The Peltier stage can offset this both by removing heat and adding heat as
> needed. A well tuned PID controller will keep the sample temperature
> within the exacting parameters you described. The stage we've used is the
> Physitemp TS-4SMP, which can both regulate the temperature of 35mm dishes
> as well as slides (for microfluidics, etc.):
> http://www.physitemp.com/products/HeatingandCooling4Scopes/
>
> Hope this helps,
> Ben Smith
>
>
>
> ________________________________________
> From: Confocal Microscopy List <[hidden email]> on
> behalf of Andreas Bruckbauer <[hidden email]>
> Sent: Friday, April 29, 2016 4:29:52 AM
> To: [hidden email]
> Subject: Re: Temperature logger for microscope enclosure
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> This might do the job with 0.015C accuracy (no commercial interest)
> https://www.picotech.com/data-logger/pt-104/high-accuracy-temperature-daq
>
>
>
> best wishes
>
>
> Andreas
>
>
>
>
>
>
>
>
>
> -----Original Message-----
> From: Julio MATEOS_LANGERAK <[hidden email]>
> To: CONFOCALMICROSCOPY <[hidden email]>
> Sent: Fri, 29 Apr 2016 9:37
> Subject: Re: Temperature logger for microscope enclosure
>
> Hi,
>
> We use across the whole facility an openHW solution provided (and also
> commercialised) at www.openenergymonitor.org. Probes (emonTH’s) are
> distributed everywhere (including our 3D-SIM enclosure) and are sensing
> temperature at regular intervals. The data is sent through radio signals to
> a base station (emonBase) on a Raspberry PI. This one collects and logs all
> the data into feeds and generates a configurable website where you may
> visualise everything. Pretty neat I have to say and very cheap compared to
> other more ‘commercial’ solutions.
>
> Best, Julio
>
> __________________________________________
> Julio Mateos Langerak, PhD.
> OMX manager
> Arnaud de Villeneuve Campus Imaging Facility
> Montpellier RIO Imaging
> Montpellier BIOCAMPUS, UMS3426
> Institut de Génétique Humaine-CNRS
> 141, rue de la Cardonille
> F-34396 Montpellier (France)
> e-mail: [hidden email]
> phone: +33.4.34.35.99.90
> fax: +33.4.34.35.99.01
> URL: http://www.mri.cnrs.fr/
> __________________________________________
>
>
> > On 28 Apr 2016, at 28 Apr-19:01, Seamus Holden <
> [hidden email]> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear colleagues
> >
> > I am looking for a fairly high sensitivity thermometer with probe to
> monitor the temperature of the microscope enclosure for our SIM microscope.
> It should be decent precision, +/-0.05C, so my understanding is that a
> thermocouple based sensor won't cut it. Ideally it should have some logging
> capability. Does anyone have any recommendations of suitable equipment?
> >
> > Many thanks,
> > Seamus
> >
> > Dr Seamus Holden
> > University Research Fellow
> >
> > Centre for Bacterial Cell Biology
> > Baddiley-Clark Building
> > Newcastle University
> > Richardson Road
> > Newcastle upon Tyne
> > NE2 4AX, United Kingdom
> >
> > Phone: +44 (0)191 208 3230
>