Tetraspeck beads for PSFs

[Jean Ross]

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Hi Everyone,

I have been preparing 0.1um Tetraspeck bead slides to use when measuring
point spread functions on our Zeiss Elyra PS1 super resolution microscope
and Zeiss LSM880 confocal with Airyscan.   The problem is that over time
the beads appear to swell to about 3 times in size and lose much of their
fluorescence.  I have tried many different mounting medias (hardening and
nonhardening) all with the same result.  Has anyone else experienced this
problem?  Any suggestions to solve the problem would be appreciated.

Thanks,
Jean

--
Jean Ross
Delaware Biotechnology Institute, BioImaging Center
University of Delaware
15 Innovation Way
Suite 117
Newark, DE  19711
Phone:  [phone]
Fax:  [phone]

[Heddleston, John]

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Hi Jean,
Over what time frame do you see them swell? In general we make bead slides relatively fresh (every ~2-3 weeks) to make sure the beads are good quality for PSF and alignment measurements.

Cheers,
John

John M. Heddleston, PhD
Applications Scientist
Advanced Imaging Center at HHMI Janelia
Ashburn, Virginia, USA

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-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Jean Ross
Sent: Friday, August 3, 2018 3:49 PM
To: [hidden email]
Subject: Tetraspeck beads for PSFs

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Everyone,

I have been preparing 0.1um Tetraspeck bead slides to use when measuring point spread functions on our Zeiss Elyra PS1 super resolution microscope
and Zeiss LSM880 confocal with Airyscan.   The problem is that over time
the beads appear to swell to about 3 times in size and lose much of their fluorescence.  I have tried many different mounting medias (hardening and
nonhardening) all with the same result.  Has anyone else experienced this problem?  Any suggestions to solve the problem would be appreciated.

Thanks,
Jean

--
Jean Ross
Delaware Biotechnology Institute, BioImaging Center University of Delaware
15 Innovation Way
Suite 117
Newark, DE  19711
Phone:  [phone]
Fax:  [phone]