I am doing this as we speak, and find that a final dilution somewhere
between 1/100 and 1/1000 is good, depending on how long I want to
search for the perfect bead and the field of view. They seem to be
much less concentrated than normal fluorescent beads.
Tim O'Brien
UNC Physics and Astronomy/CISMM
Claire Brown, Dr. wrote:
I’m making some tetraspeck bead samples and also
some
calibration bead samples. I was wondering if anyone has worked out an
ideal
dilution for PSF imaging so that there is a good density of beads to
ensure
single beads but lots in a single field of view. I have worked out
conditions
for the Invitrogen single coloured 0.1 um beads I could share in return.
Sincerely,
Claire
****NOTE
MY NEW ADDRESS****
Claire
M. Brown, PhD
Life
Sciences Complex Imaging Facility Director
McGill
University Department of Biochemistry
Locked
