Thanks for objective Adaptor Information!

[O'Malley, Donald]

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I got good leads on finding an RMS to Nikon adaptor (via Thor labs), as well as good advice on trying this.

Thanks everyone,
Don

Don O'Malley
Dept. Biology
[hidden email]<mailto:[hidden email]>
617-373-2284

[Brad Bobbitt]

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  This Thor part will work great. I just wanted to caution you to  
lightly oil all threads so that the adapter does not seize onto the  
objective or microscope revolver. There are notches on one side of the  
adapter. Point them towards the objective. If the adapter gets stuck  
in the scope, you can use a spanner wrench to remove it if the notches  
are facing up. If the adapter gets stuck to objective, you can grab it  
with a wrench to unscrew it and will only risk ruining the $12 adapter  
and not your objective. It is really easy to get these adapters stuck  
if they are dry so just be sure to lightly lubricate. I work with  
these often and just wanted to pass along these tips.

Brad Bobbitt
LSM Tech, LLC
www.Lsmtech.com
[hidden email]

[Paul Rigby-2]

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Hi All,
Great advice Brad, particularly if there is a chance of a liquid spill around the objective (when doing live cell imaging).

However, can you suggest suitable lubricants please. I can just imagine someone using oil that is slightly volatile (particularly if using an objective heater) - the result is likely a thin film of oil all over the optics. What do you use?

Cheers
Paul Rigby

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Brad Bobbitt
Sent: Wednesday, 16 February 2011 11:49 AM
To: [hidden email]
Subject: Thor labs RMS/M25 adapter

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  This Thor part will work great. I just wanted to caution you to  
lightly oil all threads so that the adapter does not seize onto the  
objective or microscope revolver. There are notches on one side of the  
adapter. Point them towards the objective. If the adapter gets stuck  
in the scope, you can use a spanner wrench to remove it if the notches  
are facing up. If the adapter gets stuck to objective, you can grab it  
with a wrench to unscrew it and will only risk ruining the $12 adapter  
and not your objective. It is really easy to get these adapters stuck  
if they are dry so just be sure to lightly lubricate. I work with  
these often and just wanted to pass along these tips.

Brad Bobbitt
LSM Tech, LLC
www.Lsmtech.com
[hidden email]

[Craig Brideau]

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I use either light 3-in-1 machine oil, or even Type B lens oil sparingly
applied works well.  Just be careful not to get it on any knurled surfaces
that you grip or it will make it harder to unscrew the adapter in the
future!

Craig


On Tue, Feb 15, 2011 at 9:19 PM, Paul Rigby <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi All,
> Great advice Brad, particularly if there is a chance of a liquid spill
> around the objective (when doing live cell imaging).
>
> However, can you suggest suitable lubricants please. I can just imagine
> someone using oil that is slightly volatile (particularly if using an
> objective heater) - the result is likely a thin film of oil all over the
> optics. What do you use?
>
> Cheers
> Paul Rigby
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Brad Bobbitt
> Sent: Wednesday, 16 February 2011 11:49 AM
> To: [hidden email]
> Subject: Thor labs RMS/M25 adapter
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
>  This Thor part will work great. I just wanted to caution you to
> lightly oil all threads so that the adapter does not seize onto the
> objective or microscope revolver. There are notches on one side of the
> adapter. Point them towards the objective. If the adapter gets stuck
> in the scope, you can use a spanner wrench to remove it if the notches
> are facing up. If the adapter gets stuck to objective, you can grab it
> with a wrench to unscrew it and will only risk ruining the $12 adapter
> and not your objective. It is really easy to get these adapters stuck
> if they are dry so just be sure to lightly lubricate. I work with
> these often and just wanted to pass along these tips.
>
> Brad Bobbitt
> LSM Tech, LLC
> www.Lsmtech.com
> [hidden email]
>