Thick, low cost fluorescent or auto-fluorescent slides / samples?
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WAINWRIGHT James |
Thick, low cost fluorescent or auto-fluorescent slides / samples?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear mailing list, I'm trying to find some low cost prepared slides that meet the following criteria: 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. 2) Quite thick - at least 100 µm, ideally 200 µm or more. 3) Low cost - i.e. ~£10 per slide. Cheaper = better. The most success I've had so far is with pollen grain prepared slides - these are wonderfully bright under fluorescence, detailed, low cost, but not very thick (typically up to 50 µm). Plant cross-sections such as Convallaria are also great, but again, tend to only be 10 µm or so thick. Many thanks for any ideas or suppliers you can provide, on or off-list. James ___________________________________________________________________________This e-mail is confidential and is for the addressee only. Please refer to www.oxinst.com/email-statement for regulatory information. |
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George McNamara |
Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides (you should give them your shipping number to send to you). I believe 1 mm thick (maybe more). You can also visit their booth at the next trade show (Biophysics?). sincerely, George On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear mailing list, > > I'm trying to find some low cost prepared slides that meet the following criteria: > > > 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. > > 2) Quite thick - at least 100 µm, ideally 200 µm or more. > > 3) Low cost - i.e. ~£10 per slide. Cheaper = better. > > The most success I've had so far is with pollen grain prepared slides - these are wonderfully bright under fluorescence, detailed, low cost, but not very thick (typically up to 50 µm). Plant cross-sections such as Convallaria are also great, but again, tend to only be 10 µm or so thick. > > Many thanks for any ideas or suppliers you can provide, on or off-list. > > James > > > > ___________________________________________________________________________This e-mail is confidential and is for the addressee only. Please refer to www.oxinst.com/email-statement for regulatory information. -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 |
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John Oreopoulos |
Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I think he's looking for something with 3D structure, George, rather than just a fluorescent slab. John Oreopoulos > On Jan 30, 2017, at 8:43 AM, George McNamara <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi James, > > ask Chroma for a box, or a case, of their fluorescent plastic slides (you should give them your shipping number to send to you). I believe 1 mm thick (maybe more). > > You can also visit their booth at the next trade show (Biophysics?). > > > sincerely, > > George > > >> On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Dear mailing list, >> >> I'm trying to find some low cost prepared slides that meet the following criteria: >> >> >> 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. >> >> 2) Quite thick - at least 100 µm, ideally 200 µm or more. >> >> 3) Low cost - i.e. ~£10 per slide. Cheaper = better. >> >> The most success I've had so far is with pollen grain prepared slides - these are wonderfully bright under fluorescence, detailed, low cost, but not very thick (typically up to 50 µm). Plant cross-sections such as Convallaria are also great, but again, tend to only be 10 µm or so thick. >> >> Many thanks for any ideas or suppliers you can provide, on or off-list. >> >> James >> >> >> >> ___________________________________________________________________________This e-mail is confidential and is for the addressee only. Please refer to www.oxinst.com/email-statement for regulatory information. > > -- > > > George McNamara, PhD > Houston, TX 77054 > [hidden email] > https://www.linkedin.com/in/georgemcnamara > https://works.bepress.com/gmcnamara/75/ > http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 |
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Gary Laevsky |
Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Brine shrimp? On Mon, Jan 30, 2017 at 9:03 AM, John Oreopoulos < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > I think he's looking for something with 3D structure, George, rather than > just a fluorescent slab. > > John Oreopoulos > > > On Jan 30, 2017, at 8:43 AM, George McNamara <[hidden email]> > wrote: > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Hi James, > > > > ask Chroma for a box, or a case, of their fluorescent plastic slides > (you should give them your shipping number to send to you). I believe 1 mm > thick (maybe more). > > > > You can also visit their booth at the next trade show (Biophysics?). > > > > > > sincerely, > > > > George > > > > > >> On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> Post images on http://www.imgur.com and include the link in your > posting. > >> ***** > >> > >> Dear mailing list, > >> > >> I'm trying to find some low cost prepared slides that meet the > following criteria: > >> > >> > >> 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. > >> > >> 2) Quite thick - at least 100 µm, ideally 200 µm or more. > >> > >> 3) Low cost - i.e. ~£10 per slide. Cheaper = better. > >> > >> The most success I've had so far is with pollen grain prepared slides - > these are wonderfully bright under fluorescence, detailed, low cost, but > not very thick (typically up to 50 µm). Plant cross-sections such as > Convallaria are also great, but again, tend to only be 10 µm or so thick. > >> > >> Many thanks for any ideas or suppliers you can provide, on or off-list. > >> > >> James > >> > >> > >> > >> ___________________________________________________________________________This > e-mail is confidential and is for the addressee only. Please refer to > www.oxinst.com/email-statement for regulatory information. > > > > -- > > > > > > George McNamara, PhD > > Houston, TX 77054 > > [hidden email] > > https://www.linkedin.com/in/georgemcnamara > > https://works.bepress.com/gmcnamara/75/ > > http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 > -- Best, Gary Laevsky, Ph.D. Director, Confocal Imaging Facility Nikon Center of Excellence Dept. of Molecular Biology Washington Rd. Princeton University Princeton, New Jersey, 08544-1014 (O) 609 258 5432 (C) 508 507 1310 |
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WAINWRIGHT James |
Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Thanks Gary - I'll give them a try. Yes - I'm after something with structure, rather than the Chroma plastic slides. James -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Gary Laevsky Sent: 30 January 2017 14:11 To: [hidden email] Subject: Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples? EXTERNAL EMAIL ATTACHMENT ADVISORY ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Brine shrimp? On Mon, Jan 30, 2017 at 9:03 AM, John Oreopoulos < [hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > I think he's looking for something with 3D structure, George, rather > than just a fluorescent slab. > > John Oreopoulos > > > On Jan 30, 2017, at 8:43 AM, George McNamara > > <[hidden email]> > wrote: > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Hi James, > > > > ask Chroma for a box, or a case, of their fluorescent plastic slides > (you should give them your shipping number to send to you). I believe > 1 mm thick (maybe more). > > > > You can also visit their booth at the next trade show (Biophysics?). > > > > > > sincerely, > > > > George > > > > > >> On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> Post images on http://www.imgur.com and include the link in your > posting. > >> ***** > >> > >> Dear mailing list, > >> > >> I'm trying to find some low cost prepared slides that meet the > following criteria: > >> > >> > >> 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. > >> > >> 2) Quite thick - at least 100 µm, ideally 200 µm or more. > >> > >> 3) Low cost - i.e. ~£10 per slide. Cheaper = better. > >> > >> The most success I've had so far is with pollen grain prepared > >> slides - > these are wonderfully bright under fluorescence, detailed, low cost, > but not very thick (typically up to 50 µm). Plant cross-sections such > as Convallaria are also great, but again, tend to only be 10 µm or so thick. > >> > >> Many thanks for any ideas or suppliers you can provide, on or off-list. > >> > >> James > >> > >> > >> > >> ___________________________________________________________________ > >> ________This > e-mail is confidential and is for the addressee only. Please refer to > www.oxinst.com/email-statement for regulatory information. > > > > -- > > > > > > George McNamara, PhD > > Houston, TX 77054 > > [hidden email] > > https://www.linkedin.com/in/georgemcnamara > > https://works.bepress.com/gmcnamara/75/ > > http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 > -- Best, Gary Laevsky, Ph.D. Director, Confocal Imaging Facility Nikon Center of Excellence Dept. of Molecular Biology Washington Rd. Princeton University Princeton, New Jersey, 08544-1014 (O) 609 258 5432 (C) 508 507 1310 +++Scanned for Viruses by ForcePoint+++ |
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Leddon, Scott |
Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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In reply to this post by WAINWRIGHT James
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Instead of pollen, how about pollen with the flower still around it? Such as https://www.flickr.com/photos/mcammer/13745935665/ https://www.flickr.com/photos/mcammer/12177302295/ https://www.flickr.com/photos/mcammer/10874392726/ ========================================================================= Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center Cell: 914-309-3270 Office: Skirball 2nd Floor main office, back right http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of WAINWRIGHT James Sent: Monday, January 30, 2017 8:09 AM To: [hidden email] Subject: Thick, low cost fluorescent or auto-fluorescent slides / samples? ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DgIFAw&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=eFeCDRa45fhpnVsvuQFfRh4aUaXbugy2PqdF-_9gcz0&s=kcWNv1Xzowu3gNHgNM651RYhmnqEmogl0LXcFdQIEes&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DgIFAw&c=j5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=oU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=eFeCDRa45fhpnVsvuQFfRh4aUaXbugy2PqdF-_9gcz0&s=rGCGUsUZqgwH3EQenKzzH1jU1TrKipf6MUwQZs2nS-o&e= and include the link in your posting. ***** Dear mailing list, I'm trying to find some low cost prepared slides that meet the following criteria: 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. 2) Quite thick - at least 100 µm, ideally 200 µm or more. 3) Low cost - i.e. ~£10 per slide. Cheaper = better. The most success I've had so far is with pollen grain prepared slides - these are wonderfully bright under fluorescence, detailed, low cost, but not very thick (typically up to 50 µm). Plant cross-sections such as Convallaria are also great, but again, tend to only be 10 µm or so thick. Many thanks for any ideas or suppliers you can provide, on or off-list. James ___________________________________________________________________________This e-mail is confidential and is for the addressee only. Please refer to www.oxinst.com/email-statement for regulatory information. ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= |
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George McNamara |
scratch top and bottom of Chroma slide ... Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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In reply to this post by John Oreopoulos
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** scratch top and bottom of Chroma slide. Sparse shallow and/or deep. For more fun, sharpie marks on the surface and in the trenches. Constellation beads (or their successors) to add more colors to each slide. On 1/30/2017 8:03 AM, John Oreopoulos wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > I think he's looking for something with 3D structure, George, rather than just a fluorescent slab. > > John Oreopoulos > >> On Jan 30, 2017, at 8:43 AM, George McNamara <[hidden email]> wrote: >> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Hi James, >> >> ask Chroma for a box, or a case, of their fluorescent plastic slides (you should give them your shipping number to send to you). I believe 1 mm thick (maybe more). >> >> You can also visit their booth at the next trade show (Biophysics?). >> >> >> sincerely, >> >> George >> >> >>> On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images on http://www.imgur.com and include the link in your posting. >>> ***** >>> >>> Dear mailing list, >>> >>> I'm trying to find some low cost prepared slides that meet the following criteria: >>> >>> >>> 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. >>> >>> 2) Quite thick - at least 100 µm, ideally 200 µm or more. >>> >>> 3) Low cost - i.e. ~£10 per slide. Cheaper = better. >>> >>> The most success I've had so far is with pollen grain prepared slides - these are wonderfully bright under fluorescence, detailed, low cost, but not very thick (typically up to 50 µm). Plant cross-sections such as Convallaria are also great, but again, tend to only be 10 µm or so thick. >>> >>> Many thanks for any ideas or suppliers you can provide, on or off-list. >>> >>> James >>> >>> >>> >>> ___________________________________________________________________________This e-mail is confidential and is for the addressee only. Please refer to www.oxinst.com/email-statement for regulatory information. >> -- >> >> >> George McNamara, PhD >> Houston, TX 77054 >> [hidden email] >> https://www.linkedin.com/in/georgemcnamara >> https://works.bepress.com/gmcnamara/75/ >> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 |
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Romain Laine |
Re: scratch top and bottom of Chroma slide ... Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear all, This paper might be of interest for this http://journal.frontiersin.org/article/10.3389/fphys.2014.00384/abstract Martin Booth, Tony Wilson and Alex Corbett They actually create structures from Chroma slides by laser writing using non-linear absorption. Cheers, Romain On Mon, Jan 30, 2017 at 6:02 PM, George McNamara <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > scratch top and bottom of Chroma slide. Sparse shallow and/or deep. For > more fun, sharpie marks on the surface and in the trenches. Constellation > beads (or their successors) to add more colors to each slide. > > On 1/30/2017 8:03 AM, John Oreopoulos wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> I think he's looking for something with 3D structure, George, rather than >> just a fluorescent slab. >> >> John Oreopoulos >> >> On Jan 30, 2017, at 8:43 AM, George McNamara <[hidden email]> >>> wrote: >>> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images on http://www.imgur.com and include the link in your >>> posting. >>> ***** >>> >>> Hi James, >>> >>> ask Chroma for a box, or a case, of their fluorescent plastic slides >>> (you should give them your shipping number to send to you). I believe 1 mm >>> thick (maybe more). >>> >>> You can also visit their booth at the next trade show (Biophysics?). >>> >>> >>> sincerely, >>> >>> George >>> >>> >>> On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> Post images on http://www.imgur.com and include the link in your >>>> posting. >>>> ***** >>>> >>>> Dear mailing list, >>>> >>>> I'm trying to find some low cost prepared slides that meet the >>>> following criteria: >>>> >>>> >>>> 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. >>>> >>>> 2) Quite thick - at least 100 µm, ideally 200 µm or more. >>>> >>>> 3) Low cost - i.e. ~£10 per slide. Cheaper = better. >>>> >>>> The most success I've had so far is with pollen grain prepared slides - >>>> these are wonderfully bright under fluorescence, detailed, low cost, but >>>> not very thick (typically up to 50 µm). Plant cross-sections such as >>>> Convallaria are also great, but again, tend to only be 10 µm or so thick. >>>> >>>> Many thanks for any ideas or suppliers you can provide, on or off-list. >>>> >>>> James >>>> >>>> >>>> >>>> ___________________________________________________________________________This >>>> e-mail is confidential and is for the addressee only. Please refer to >>>> www.oxinst.com/email-statement for regulatory information. >>>> >>> -- >>> >>> >>> George McNamara, PhD >>> Houston, TX 77054 >>> [hidden email] >>> https://www.linkedin.com/in/georgemcnamara >>> https://works.bepress.com/gmcnamara/75/ >>> http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 >>> >> > -- > > > George McNamara, PhD > Houston, TX 77054 > [hidden email] > https://www.linkedin.com/in/georgemcnamara > https://works.bepress.com/gmcnamara/75/ > http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 > |
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Steffen Dietzel |
Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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In reply to this post by WAINWRIGHT James
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** James, how about thicker pollen grains? This page gives a list of plants with sizes over 100 µm; http://pollen.tstebler.ch/MediaWiki/index.php?title=Kategorie:SizeVeryLarge While the site is in German, it gives the scientific names, so you should be fine. I did check for /Hibiscus /and /Cucurbita/, both are indeed very large, up to 150 µm maybe. I found it much easier to immerse them in immersion oil rather than water, probably they have hydrophobic surfaces. Plus, it helps for high resolution at these distances from the coverslip. Else, you can use a sharp knife and cut yourself a piece of a wooden sprout from pretty much any plant and embed it in glycerol or immersion oil, nailpolish around the coverglass, or with your favorite hardening mounting medium. Wood is usually autofluorescent, some species quite beautifully so. For example, check out this page from the German hobby-microscopist forum: http://www.mikroskopie-forum.de/index.php?topic=27428.msg206927#msg206927 Scroll down for microscopic images, they are from /Robinia /and /Rhus typhina. Cornus/ is also said to be very nice. I am sure there are more examples also at other language microphotography forums. The people there might be helpful to figure out which species are best to use in your geographic area. A third option are Lichen, also often very fluorescent and they show nice 3D structures on the surface. I use a piece of a dried branch with lichen on it as test sample for our stereomicroscope. If you carefully scratch the lichen off and transfer them on a slide, it should make a nice preparation. If you have a portable UV light (Amazon..), it may be worth going through a dark forest or garden at night to find the right ones. If you have kids, take them with you :-) Good hunting, Steffen Am 30.01.2017 um 14:09 schrieb WAINWRIGHT James: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear mailing list, > > I'm trying to find some low cost prepared slides that meet the following criteria: > > > 1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. > > 2) Quite thick - at least 100 µm, ideally 200 µm or more. > > 3) Low cost - i.e. ~£10 per slide. Cheaper = better. > > The most success I've had so far is with pollen grain prepared slides - these are wonderfully bright under fluorescence, detailed, low cost, but not very thick (typically up to 50 µm). Plant cross-sections such as Convallaria are also great, but again, tend to only be 10 µm or so thick. > > Many thanks for any ideas or suppliers you can provide, on or off-list. > > James > > > > ___________________________________________________________________________This e-mail is confidential and is for the addressee only. Please refer to www.oxinst.com/email-statement for regulatory information. > -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany http://www.bioimaging.bmc.med.uni-muenchen.de |
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Barbara Foster |
Re: scratch top and bottom of Chroma slide ... Re: Hi James, ask Chroma for a box, or a case, of their fluorescent plastic slides ... Re: Thick, low cost fluorescent or auto-fluorescent slides / samples?
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In reply to this post by George McNamara
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Just a reminder that MicroscopyEducation.com also carries a set of fluorescence reference slides, developed by P. C. Cheng at SUNY/Buffalo: http://microscopyeducation.com/fluorref-slides/ **Caveat: Commercial posting Good hunting! Barbara Foster, President & Chief Consultant Microscopy/Microscopy Education ... "Education, not Training" 7101 Royal Glen Trail, Suite A - McKinney, TX 75070 - P: 972-924-5310 www.MicroscopyEducation.com Microscopy/Microscopy Education is a division of The Microscopy & Imaging Place, Inc. At 12:02 PM 1/30/2017, you wrote: >***** >To join, leave or search the confocal microscopy listserv, go to: >http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >Post images on http://www.imgur.com and include the link in your posting. >***** > >scratch top and bottom of Chroma slide. Sparse >shallow and/or deep. For more fun, sharpie marks >on the surface and in the trenches. >Constellation beads (or their successors) to add more colors to each slide. > >On 1/30/2017 8:03 AM, John Oreopoulos wrote: >>***** >>To join, leave or search the confocal microscopy listserv, go to: >>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>Post images on http://www.imgur.com and include the link in your posting. >>***** >> >>I think he's looking for something with 3D >>structure, George, rather than just a fluorescent slab. >> >>John Oreopoulos >> >>>On Jan 30, 2017, at 8:43 AM, George McNamara >>><[hidden email]> wrote: >>> >>>***** >>>To join, leave or search the confocal microscopy listserv, go to: >>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>Post images on http://www.imgur.com and include the link in your posting. >>>***** >>> >>>Hi James, >>> >>>ask Chroma for a box, or a case, of their >>>fluorescent plastic slides (you should give >>>them your shipping number to send to you). I believe 1 mm thick (maybe more). >>> >>>You can also visit their booth at the next trade show (Biophysics?). >>> >>> >>>sincerely, >>> >>>George >>> >>> >>>>On 1/30/2017 7:09 AM, WAINWRIGHT James wrote: >>>>***** >>>>To join, leave or search the confocal microscopy listserv, go to: >>>>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>Post images on http://www.imgur.com and include the link in your posting. >>>>***** >>>> >>>>Dear mailing list, >>>> >>>>I'm trying to find some low cost prepared >>>>slides that meet the following criteria: >>>> >>>> >>>>1) Fluorescent, or auto-fluorescent with UV/VIS/NIR. >>>> >>>>2) Quite thick - at least 100 µm, ideally 200 µm or more. >>>> >>>>3) Low cost - i.e. ~£10 per slide. Cheaper = better. >>>> >>>>The most success I've had so far is with >>>>pollen grain prepared slides - these are >>>>wonderfully bright under fluorescence, >>>>detailed, low cost, but not very thick >>>>(typically up to 50 µm). Plant >>>>cross-sections such as Convallaria are also >>>>great, but again, tend to only be 10 µm or so thick. >>>> >>>>Many thanks for any ideas or suppliers you can provide, on or off-list. >>>> >>>>James >>>> >>>> >>>> >>>>___________________________________________________________________________This >>>>e-mail is confidential and is for the >>>>addressee only. Please refer to >>>>www.oxinst.com/email-statement for regulatory information. >>>-- >>> >>> >>>George McNamara, PhD >>>Houston, TX 77054 >>>[hidden email] >>>https://www.linkedin.com/in/georgemcnamara >>>https://works.bepress.com/gmcnamara/75/ >>>http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 > >-- > > >George McNamara, PhD >Houston, TX 77054 >[hidden email] >https://www.linkedin.com/in/georgemcnamara >https://works.bepress.com/gmcnamara/75/ >http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 |
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