Thoughts on Axioimager line of Microscopes/ Imaging system
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Farid Jalali |
Thoughts on Axioimager line of Microscopes/ Imaging system
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Hello All,
I would appreciate hearing thoughts on Zeiss' line of Axioimager imaging systems. I've just found out that an A1 (most basic, for documentation) is going into our tiny (2 scope) facility. However, I am thinking of asking for an upgrade to the M1, motorized and apparently suitable for live cell imaging, 3D deconvolution microscopy, FRET even. Is anyone out there using their Axiovision's for live cell imaging, FRET, 3D acquisition and deconvolution?
Any and all thoughts are greatly appreciated.
Cheers
Farid
-- Farid Jalali MSc Senior Research Technician/ Lab Manager Dr. Robert Bristow Lab Applied Molecular Oncology Princess Margaret Hospital Toronto, Canada 416-946-4501 X4351 (Princess Margaret Hospital) 416-581-7754 STTARR at MaRS Building 416-581-7791 STTARR Microscopy Suite |
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Armstrong, Brian |
Re: Thoughts on Axioimager line of Microscopes/ Imaging system
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Hi Farid, we just received an Observer Z1
with the intention of setting up a live-cell imaging station. We do not yet
have our water immersion objectives or our C9100-13 EMCCD, so I cannot comment
on functionality as of yet. However, I would certainly get the M1 and would get
the Z1 if you can. I know these get expensive real quick, but I think you will thank
yourself for the scanning stage later. My idea is to use the docking station
away from the scope free from vibration and interruption as we acquire in an
automated motorized fashion within an enclosed microscope incubator. Reaching
in the incubator hundreds of times does not sound very appealing for live-cell
imaging. Moreover, I think you will use the motorized functions for
autofocusing and the like during long-term acquisitions. Furthermore, it is the
motorized components that really supply the functionality to the docking
station. Cheers, Brian D Armstrong PhD Light Microscopy Core Manager Beckman Research Institute City of 626-359-8111 x62872 From: Confocal
Microscopy List [mailto:[hidden email]] On Behalf Of Farid Jalali Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello All, I would appreciate hearing thoughts on Zeiss' line of Axioimager
imaging systems. I've just found out that an A1 (most basic, for
documentation) is going into our tiny (2 scope) facility. However, I am
thinking of asking for an upgrade to the M1, motorized and apparently suitable
for live cell imaging, 3D deconvolution microscopy, FRET even. Is anyone out
there using their Axiovision's for live cell imaging, FRET, 3D acquisition and
deconvolution? Any and all thoughts are greatly appreciated. Cheers Farid
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In reply to this post by Farid Jalali
Search the CONFOCAL archive at
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I recently built an A1 using Ludl XY stage
and Z motor, all controlled through Ludl MAC 5000 controler. We use the system
for stereology. If you don’t get the upgrade, the A1 is still a very
flexible stand. Because we cover a lot of disciplines here, our A1 was built to
be capable of tiling, z-stacking, time series, transmitted light imaging, polarized
light imaging, fluorescence imaging (Ludle 10 port filter wheels) and reflected
light imaging. If you go with the motorized versions and add the Axiovision
software for control along with Zeiss cameras I think you will be quite happy. We have an inverted Axiovert 200M that can
do limited live cell work; it also does everything the A1 does, except it only
has five fluorescent channels (no filter wheels) and uses Axiovision software. The
key to success with the Axiovision software is to buy the Zeiss cameras also.
We have a Roper EMCCD and a Zeiss Axiocam and there are features like shading
correction that are locked out of the multidimensional acquisition setup in for
the Roper camera making it harder to work with than the Zeiss camera. You can
still do shading correction it just takes more steps. Both A1 and the Axiovert 200M are used
quite heavily and require little maintenance. When a part does break it can be
exchanged at a significantly reduced cost through a parts exchange program. Cheers, Jonathan M. Ekman Imaging Technology Group Beckman Institute for
Advanced Science and Tel: 217-244-6292 Fax: 217-244-6219 From: Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello All, I would appreciate hearing thoughts on Zeiss' line of Axioimager
imaging systems. I've just found out that an A1 (most basic, for
documentation) is going into our tiny (2 scope) facility. However, I am
thinking of asking for an upgrade to the M1, motorized and apparently suitable
for live cell imaging, 3D deconvolution microscopy, FRET even. Is anyone out
there using their Axiovision's for live cell imaging, FRET, 3D acquisition and
deconvolution? Any and all thoughts are greatly appreciated. Cheers Farid
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Armstrong, Brian |
Re: Thoughts on Axioimager line of Microscopes/ Imaging system
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
Hi John, I have always had a high opinion
of LUDL products. Can you give an idea of how much this A1 LUDL set up cost
you? Is it more cost effective to get the motorized components from Zeiss or
LUDL? Is your camera the Photometrics QuantEM?
How are you liking the EM camera (other than the problems with Axiovision
driver compatability)? Cheers, Brian D Armstrong PhD Light Microscopy Core Manager Beckman Research Institute City of 626-359-8111 x62872 From: Confocal
Microscopy List [mailto:[hidden email]] On Behalf Of Jon Ekman I recently built an A1 using Ludl XY stage
and Z motor, all controlled through Ludl MAC 5000 controler. We use the system
for stereology. If you don’t get the upgrade, the A1 is still a very
flexible stand. Because we cover a lot of disciplines here, our A1 was built to
be capable of tiling, z-stacking, time series, transmitted light imaging,
polarized light imaging, fluorescence imaging (Ludle 10 port filter wheels) and
reflected light imaging. If you go with the motorized versions and add the
Axiovision software for control along with Zeiss cameras I think you will be
quite happy. We have an inverted Axiovert 200M that can
do limited live cell work; it also does everything the A1 does, except it only has
five fluorescent channels (no filter wheels) and uses Axiovision software. The
key to success with the Axiovision software is to buy the Zeiss cameras also.
We have a Roper EMCCD and a Zeiss Axiocam and there are features like shading
correction that are locked out of the multidimensional acquisition setup in for
the Roper camera making it harder to work with than the Zeiss camera. You can
still do shading correction it just takes more steps. Both A1 and the Axiovert 200M are used
quite heavily and require little maintenance. When a part does break it can be
exchanged at a significantly reduced cost through a parts exchange program. Cheers, Jonathan M. Ekman Imaging Technology Group Beckman Institute for
Advanced Science and Tel: 217-244-6292 Fax: 217-244-6219 From: Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello All, I would appreciate hearing thoughts on Zeiss' line of Axioimager
imaging systems. I've just found out that an A1 (most basic, for
documentation) is going into our tiny (2 scope) facility. However, I am
thinking of asking for an upgrade to the M1, motorized and apparently suitable
for live cell imaging, 3D deconvolution microscopy, FRET even. Is anyone out
there using their Axiovision's for live cell imaging, FRET, 3D acquisition and
deconvolution? Any and all thoughts are greatly appreciated. Cheers Farid
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
The newer LUDL stages and Z drives are
great. The small footprint of the Mac5000 is nice too. The difference between
Zeiss and LUDL is that during acquisition of Z stacks we have to time the acquisition
to adjust for settlement of LUDL Z motor after it moves a step in Z. If we don’t
account for the adjustment we see jumping when we play back the Z-stack. We do
not have this problem with the Axiovert 200m’s z-drive, and I suspect you
will not see it on the motorized Axioimager stands either. We bought all the LUDL parts (Mac 5000,
focus encoder, joystick, scanning stage, z-motor and filter wheels) as part of
a package from a software vender (MBF Bioscience, makers of SteroInvestigator
and Neurolucida) and then put it all together on the Axioimager A1. We are very
happy with it. There was no real cost benefit between all Zeiss + Marzhauser
scanning stage and MCU 28 controller and the LUDL setup. The big difference is
that the Axiovision software has more refined control over the hardware and
does just about everything we need, imaging wise. MBF bioscience makes a very
specific package and its software control is focused on stereology, which we
feel they excel at. For us, we had the A1 stand already, and wanted to run StereoInvestigator
and Neurolucida on it. Maintenance wise: if the LUDL Z motor
fails, the A1 scope is still operational with manual z-control and there is easy
access to the motor and controller boards without disturbing the scope setup.
If the Z-motor fails in the Zeiss stand I believe the scope will be down until you,
or a service engineer can break down the scope to get to its internal boards. If
you ensure clean electrical current to the stand through at least a line
conditioner, and avoid soaking the system in water or culture media, the
Z-motor in the Zeiss stands should last a long time. As for cameras, on the A1, we are using an
Olympus Microfire color CCD camera with the IR blocking filter in the emission
filter wheel so we could image Cy5, on our Axiovert 200M we have an AxioCam HRc
for our histology folks, and the Roper Cascade 512B for fluorescence
imaging. We purchased the Roper Cascade 512B EMCCD first,
3 years ago, and then built a system around it; we tried software control from
a couple different venders, but found the Axiovision software to be the best
match for what we wanted out of the system. We have no driver issues with the Axiovision
software and the Roper camera any more, except that the method used for flat
field/shading correction is awkward compared to using the Zeiss camera. We have
close to 130 active users, mainly a mixed batch of biologists and engineers on
the system, the majority use the EMCCD with no complaints. We have 40 users on
the A1. Cheers, Jonathan M. Ekman Imaging Technology Group Beckman Institute for
Advanced Science and Technology Tel: 217-244-6292 Fax: 217-244-6219 From: Hi John, I have always had a high opinion
of LUDL products. Can you give an idea of how much this A1 LUDL set up cost
you? Is it more cost effective to get the motorized components from Zeiss or
LUDL? Is your camera the Photometrics QuantEM?
How are you liking the EM camera (other than the problems with Axiovision
driver compatability)? Cheers, Brian D Armstrong PhD Light Microscopy Core Manager Beckman Research Institute City of 626-359-8111 x62872 From: I recently built an A1 using Ludl XY stage
and Z motor, all controlled through Ludl MAC 5000 controler. We use the system
for stereology. If you don’t get the upgrade, the A1 is still a very
flexible stand. Because we cover a lot of disciplines here, our A1 was built to
be capable of tiling, z-stacking, time series, transmitted light imaging,
polarized light imaging, fluorescence imaging (Ludle 10 port filter wheels) and
reflected light imaging. If you go with the motorized versions and add the
Axiovision software for control along with Zeiss cameras I think you will be
quite happy. We have an inverted Axiovert 200M that can
do limited live cell work; it also does everything the A1 does, except it only
has five fluorescent channels (no filter wheels) and uses Axiovision software.
The key to success with the Axiovision software is to buy the Zeiss cameras
also. We have a Roper EMCCD and a Zeiss Axiocam and there are features like
shading correction that are locked out of the multidimensional acquisition
setup in for the Roper camera making it harder to work with than the Zeiss
camera. You can still do shading correction it just takes more steps. Both A1 and the Axiovert 200M are used
quite heavily and require little maintenance. When a part does break it can be
exchanged at a significantly reduced cost through a parts exchange program. Cheers, Jonathan M. Ekman Imaging Technology Group Beckman Institute for
Advanced Science and Tel: 217-244-6292 Fax: 217-244-6219 From: Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hello All, I would appreciate hearing thoughts on Zeiss' line of Axioimager imaging
systems. I've just found out that an A1 (most basic, for documentation) is
going into our tiny (2 scope) facility. However, I am thinking of asking for an
upgrade to the M1, motorized and apparently suitable for live cell imaging, 3D
deconvolution microscopy, FRET even. Is anyone out there using their
Axiovision's for live cell imaging, FRET, 3D acquisition and deconvolution? Any and all thoughts are greatly appreciated. Cheers Farid
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Robert Peterson-3-3 |
timelapse, time-lapse, or time lapse
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Search the CONFOCAL archive at
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Here is a question I've often wondered about. Which is it: timelapse, time-lapse, or time lapse? What do you think? Robert |
peterson.vcf (469 bytes) |
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Martin Wessendorf-2 |
Re: timelapse, time-lapse, or time lapse
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Robert Peterson wrote: > Here is a question I've often wondered about. Which is it: timelapse, > time-lapse, or time lapse? American Heritage Dictionary lists it as "time-lapse". I expect they use the hyphen because it's a compound word. Martin -- Martin Wessendorf, Ph.D. office: (612) 626-0145 Assoc Prof, Dept Neuroscience lab: (612) 624-2991 University of Minnesota Preferred FAX: (612) 624-8118 6-145 Jackson Hall, 321 Church St. SE Dept Fax: (612) 626-5009 Minneapolis, MN 55455 **MY E-MAIL ADDRESS HAS CHANGED. PLEASE USE [hidden email] ** |
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Michael Herron |
Re: timelapse, time-lapse, or time lapse
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In reply to this post by Robert Peterson-3-3
Search the CONFOCAL archive at
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Clay-mation. Actually I was wondering the same thing.
MS Word and my mac MAIL program both like time-lapse. If MS and Apple agree on something you can't go wrong!
On Feb 28, 2008, at 2:00 PM, Robert Peterson wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Michael J. Herron, U of MN, Dept. of Entomology 612-624-3688 (office) 612-625-5299 (FAX) |
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Michael Cammer |
Re: timelapse, time-lapse, or time lapse
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal At 03:56 PM 02/28/08, you wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >Clay-mation. Actually I was wondering the same thing. > > MS Word and my mac MAIL program both like time-lapse. If MS and > Apple agree on something you can't go wrong! Ok, so which is correct, Helvetica or Arial? ____________________________________________________________________________ Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. URL: http://www.aecom.yu.edu/aif/ |
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Tobias Baskin |
Re: timelapse, time-lapse, or time lapse
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Helvetica? Arial? Correct? They are both ugly (the original name for Helvetica was more or less New House Grotesque) but those names refer to different fonts. Similar to be sure but not identical. TB >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >At 03:56 PM 02/28/08, you wrote: >>Search the CONFOCAL archive at >>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >>Clay-mation. Actually I was wondering the same thing. >> >> MS Word and my mac MAIL program both like time-lapse. If MS and >>Apple agree on something you can't go wrong! > >Ok, so which is correct, Helvetica or Arial? >____________________________________________________________________________ >Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med. >URL: http://www.aecom.yu.edu/aif/ -- _ ____ __ ____ / \ / / \ / \ \ Tobias I. Baskin / / / / \ \ \ Biology Department /_ / __ /__ \ \ \__ 611 N. Pleasant St. / / / \ \ \ University of Massachusetts / / / \ \ \ Amherst, MA, 01003 / / ___ / \ \__/ \ ____ http://www.bio.umass.edu/biology/baskin/ Voice: 413 - 545 - 1533 Fax: 413 - 545 - 3243 |
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Tamara Howard |
Re: timelapse, time-lapse, or time lapse
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In reply to this post by Robert Peterson-3-3
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal The OED (on-line) claims it should be "time lapse". I'd bet that it is up to the editor of a particular manuscript, when it comes right down to it! Tamara On Thu, 28 Feb 2008 15:00:41 -0500 Robert Peterson <[hidden email]> wrote: > Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > > Here is a question I've often wondered about. Which is >it: timelapse, time-lapse, or time lapse? > > What do you think? > > Robert > > > *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** |
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Adrian Smith-6 |
Re: timelapse, time-lapse, or time lapse
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In reply to this post by Michael Cammer
Search the CONFOCAL archive at
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On 29/02/2008, at 8:27 AM, Michael Cammer wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Clay-mation. Actually I was wondering the same thing.MS Word and my mac MAIL program both like time-lapse. If MS and Apple agree on something you can't go wrong! Regards, Adrian |
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George McNamara |
Re: Thoughts on Axioimager line of Microscopes/ Imaging system
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In reply to this post by Farid Jalali
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi Farid, The deconvolution is painfully slow, especially the "best" mode. This is with the PC they sold us, 4 Gb ram, Windows XP. Even after 20 hours it may not be done. There is no way to save in progress, so either the job needs to be cancelled or no one can use the scope until the job is done (and no time estimate to completion). I like the 3D rendering module. Had a rendered dithizone fluorescent pancreatic islet for Nature Protocol's web site image of the week a couple of weeks ago. There are some strange bugs in AxioVision's (4/2007 release) macros control of my Axiovert 200M's filter cubes and shutters. George At 01:08 PM 2/27/2008, you wrote: >Search the CONFOCAL archive at >http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal >Hello All, >I would appreciate hearing thoughts on Zeiss' line of Axioimager >imaging systems. I've just found out that an A1 (most basic, for >documentation) is going into our tiny (2 scope) facility. However, I >am thinking of asking for an upgrade to the M1, motorized and >apparently suitable for live cell imaging, 3D deconvolution >microscopy, FRET even. Is anyone out there using their Axiovision's >for live cell imaging, FRET, 3D acquisition and deconvolution? > >Any and all thoughts are greatly appreciated. >Cheers >Farid > >-- >Farid Jalali MSc >Senior Research Technician/ Lab Manager >Dr. Robert Bristow Lab >Applied Molecular Oncology >Princess Margaret Hospital >Toronto, Canada >416-946-4501 X4351 (Princess Margaret Hospital) >416-581-7754 STTARR at MaRS Building >416-581-7791 STTARR Microscopy Suite George McNamara, Ph.D. University of Miami, Miller School of Medicine Image Core Miami, FL 33010 [hidden email] [hidden email] 305-243-8436 office http://home.earthlink.net/~pubspectra/ http://home.earthlink.net/~geomcnamara/ http://www.sylvester.org/research/SR_lab_analytical.asp?ana=desc (Analytical Imaging Core Facility) |
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George McNamara |
Re: timelapse, time-lapse, or time lapse
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In reply to this post by Robert Peterson-3-3
Search the CONFOCAL archive at
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Timelapse At 03:00 PM 2/28/2008, you wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal |
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Iain Miller |
Re: timelapse, time-lapse, or time lapse
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Search the CONFOCAL archive at
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The Oxford English Dictionary gives 2 definitions of a compound noun
formed from the words "time" and "lapse".
1. time lapse = a "lapse of time"; 2. time-lapse = "spec. (usu. with hyphen) attrib., designating or pertaining to a technique of taking a sequence of photographs at set intervals to record changes that take place slowly over time;" Therefore with regard to confocal microscopy "time-lapse" is the correct terminology. George McNamara wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal -- Iain Miller Dept. of Biological Sciences Ohio University Athens, OH 45701 740-593-2120 |
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jens rietdorf |
Re: Thoughts on Axioimager line of Microscopes/ Imaging system
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In reply to this post by Farid Jalali
Dear Farid,
we use AxioVision for 3D acquisition and FRET measurements. Our deco runs on a central server via Huygens remote manager, so we havnt used the Zeiss deco much. We also have an Apotome on the systems for optical sectioning. We failed to run our live cell imaging stations on AxioVision, because it wouldnt support some of our monochromators or dual camera. I have seen AxioVision performing very well for live cell imaging during courses. Users of our facility are generally quite happy using AxioVision and many prefer it over other software for ease of use. Hope that helps. Cheers, jens.
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