Time gating fluorescence signal with FPGA on NI PCIe-7852R board

[Lu Yan]

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Hi folks,

We are looking into options to do the time gating of fluorescence signal in
confocal/STED microscopy. Besides the widely used TCSPC tech, it seemed
that using FPGA on NI DAQ card is getting popular. I found in this paper
and some others as well [F. GÄorlitz et al., "A STED Microscope Designed
for Routine Biomedical Applications", Progress In Electromagnetics
Research, Vol. 147, 57(2014)], NI R series board PCIe-7852R was used, and
the on board FPGA was employed to control the time gating. I am currently
using a PCI-6353 card, and I just realized that programming the Labview
with DAQmx and R series cards are so different and they are not compatible,
so my labview code would not work with this new card. At this moment, I
find it a bit difficult to start with using PCIe-7852R, and I think it's
gonna take me a while before I can get the labview code up and running
again. But before that happens, I thought maybe I can get some help from
the list. So would anyone be willing to share some thoughts or maybe even
pieces of example labview code if you are also using a R series card to
control your scanning microscopy setup with a photon counting detector? I
will certainly look for example codes online, mainly from the NI forum but
I have a feeling that I will have better chance here on the list.

Thanks in advance,
Lu
-----------------------------------------------------
Lu Yan
Nanostructured Fibers and Nonlinear Optics Laboratory
Electrical and Computer Engineering
Boston University
8 St. Mary St., Boston, MA, 02215
617.353.0286 (office) | 617.358.5917 (lab)
[hidden email]
-----------------------------------------------------

[Lu Yan]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Just want to push this again... getting so low progress on the labview
coding with R-series to control the scanning microscope...

Thanks much,
Lu

-----------------------------------------------------
Lu Yan
Nanostructured Fibers and Nonlinear Optics Laboratory
Electrical and Computer Engineering
Boston University
8 St. Mary St., Boston, MA, 02215
617.353.0286 (office) | 617.358.5917 (lab)
[hidden email]
-----------------------------------------------------

On Thu, Feb 18, 2016 at 12:05 PM, Yan, Lu <[hidden email]> wrote:

> Hi folks,
>
> We are looking into options to do the time gating of fluorescence signal
> in confocal/STED microscopy. Besides the widely used TCSPC tech, it seemed
> that using FPGA on NI DAQ card is getting popular. I found in this paper
> and some others as well [F. GÄorlitz et al., "A STED Microscope Designed
> for Routine Biomedical Applications", Progress In Electromagnetics
> Research, Vol. 147, 57(2014)], NI R series board PCIe-7852R was used, and
> the on board FPGA was employed to control the time gating. I am currently
> using a PCI-6353 card, and I just realized that programming the Labview
> with DAQmx and R series cards are so different and they are not compatible,
> so my labview code would not work with this new card. At this moment, I
> find it a bit difficult to start with using PCIe-7852R, and I think it's
> gonna take me a while before I can get the labview code up and running
> again. But before that happens, I thought maybe I can get some help from
> the list. So would anyone be willing to share some thoughts or maybe even
> pieces of example labview code if you are also using a R series card to
> control your scanning microscopy setup with a photon counting detector? I
> will certainly look for example codes online, mainly from the NI forum but
> I have a feeling that I will have better chance here on the list.
>
> Thanks in advance,
> Lu
> -----------------------------------------------------
> Lu Yan
> Nanostructured Fibers and Nonlinear Optics Laboratory
> Electrical and Computer Engineering
> Boston University
> 8 St. Mary St., Boston, MA, 02215
> 617.353.0286 (office) | 617.358.5917 (lab)
> [hidden email]
> -----------------------------------------------------
>

[Jana Nieder]

Dear Colleagues and Friends,
I am pleased to inform you about the 14th IUVSTA School on NANO-OPTICS held at INL in Portugal from 11- 15. April.
 All details about the event can be found at:  http://nanoopticsschool.org

Please notice the exiting list of lectures and topics to be covered.

LIST OF INVITED AND CONFIREMED LECTURERS:
Thomas Klar
Richard Van Duyne
Anatoly Zayats
Christelle Prinz
Romain Quidant
Kjeld Pedersen
Petr Kuzel
D. Kasprowicz
Antonio G. Martin
Tord Wingren
Aaron Lewis

TITLES OF THE LECTURES:
"From Stimulated Emission Depletion (STED) Microscopy to STED Lithography"
"Recent Advances in Tip-Enhanced Raman Spectroscopy"
"The Principles and Applications of Plasmonics"
"Semiconductors nanowires for applications in biomedicine"
"Nano-plasmonics: Tools for the Detection and Manipulation Biomolecules"
"Up-Conversion Luminescence of rare earth doped micro-crystalline powders enhancement by silver nanoparticles"
"Ultrafast Photoconductivity in Nano-scaled systems: Contactless Investigation by Terahertz Spectroscopy"
"Resonant Magneto-plasmonic Structures: Fundamentals and Applications"
"Nonlinear Optical Spectroscopy on Nanostructures"
"Human Centric Lighting"
"Near-field Optics and Spectroscopy"


In addition to the lectures we will have INL guided laboratory tours – including the presentation of Nano-Optics related laboratories and experiments by the various researchers involved.

Looking forward to see you at Nano-Optics School in Braga.

Best wishes,

Jana

(local organiser of the school)



---------------------------------------------------
Dr. Jana B. Nieder
Ultrafast Bio- and Nanophotonics Group Leader
International Iberian Nanotechnolgy Laboratory - INL
http://inl.int/members/118