Tissue Staining

[Claire Brown]

Does anyone have a good reference for confocal users for proper staining of tissue sections, a how to or how not to type of protocol?

 

Sincerely,

 

 

Claire M. Brown

Life Sciences Complex Imaging Facility Director

[Aaron Johnson-9]

As a wet lab novice I have found the Immunohistochemistry Methods Express book edited by
Simon Renshaw to be extremely helpful:
http://books.google.com/books?
id=bT8uMgAACAAJ&dq=immunohistochemistry+renshaw&output=html_text&cd=1

Aaron

------------
Aaron Johnson
Pre-Doctoral Fellow
Dept. of Surgery-Otolaryngology
University of Wisconsin, Madison

[RICHARD BURRY]

For people new to immunocytochemistry, I have just published a book “Immunocytochemistry: A Practical Guide for Biomedical Research”.  The book explains the steps in the procedure and then gives ways to select a method, decide on labeling system and evaluate the antibodies for an experiment.  There are five Cases where problems are presented and solved.  Although published by Springer, the print copy is only about $80 (Amazon.com and enter Richard Burry) or  as a PDF on line at http://www.springer.com/life+sciences/biochemistry+%26+biophysics/book/978-1-4419-1303-6

----- Original Message -----
From: Aaron Johnson <[hidden email]>
Date: Wednesday, February 10, 2010 10:53 am
Subject: Re: Tissue Staining
To: [hidden email]

> As a wet lab novice I have found the Immunohistochemistry
> Methods Express book edited by
> Simon Renshaw to be extremely helpful:
> http://books.google.com/books?
> id=bT8uMgAACAAJ&dq=immunohistochemistry+renshaw&output=html_text&cd=1
>
> Aaron
>
> ------------
> Aaron Johnson
> Pre-Doctoral Fellow
> Dept. of Surgery-Otolaryngology
> University of Wisconsin, Madison
>
>
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Richard W. Burry, Ph.D.
Department of Neuroscience, College of Medicine
Campus Microscopy and Imaging Facility, Director
The Ohio State University
Associate Editor, Journal of Histochemistry and Cytochemistry
277 Biomedical Research Tower
460 West Twelfth Avenue
Columbus, Ohio 43210
Voice 614.292.2814  Cell 614.638.3345  Fax [phone]