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You could read
Confined activation and subdiffractive localization enables whole-cell
PAlm with genetically expressed probes
Andrew G York1, Alireza Ghitani1, Alipasha Vaziri2,4, Michael W Davidson3
& Hari Shroff1
From Hari Shroff group - who've been doing this for PALM (using 2-photon
excitation) - (I'm not an expert....)
I think he finds that Pamcherry works well
paGFP - not so good (at least for PALM) as too much activation at one go.
But he's a friendly guy and would be happy to answer your questions
Michelle
On 04/11/2011 16:03, "Claire Brown, Dr." <
[hidden email]> wrote:
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>
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>
>I wonder if anyone has any experience working with PA-GFP and PA-mCherry
>with two photon excitation?
>
>Has anyone measured the two photon spectral of these proteins? Do you
>know the ideal wavelength for activation?
>
>Any information would be appreciated.
>
>Claire M. Brown,
>McGill University Imaging Facility
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