Two-photon imaging with low NA objectives?

[stu_the_flat]

Hello

In our lab, we have a two-photon microscope that has 10x NA 0.3 and a 40x NA
0.8 objective.

Recently some people in our lab have taken to using the 10x objective. I
calculate the point spread function is going to be 18.5µm! That is not
taking into account the tissue scattering effects (that I don’t know how to
calculate)

Can this set up just be thought of taking very broad optical section system
or are there other things to take into consideration?

Many thanks

Stuart

[Peter Gabriel Pitrone]

Hey Stu,

Buy a 10x/0.45 or Pete's sake!

Pete

On Apr 29, 2010, at 12:50 PM, stu_the_flat wrote:

> Hello
>
> In our lab, we have a two-photon microscope that has 10x NA 0.3 and a 40x NA
> 0.8 objective.
>
> Recently some people in our lab have taken to using the 10x objective. I
> calculate the point spread function is going to be 18.5µm! That is not
> taking into account the tissue scattering effects (that I don’t know how to
> calculate)
>
> Can this set up just be thought of taking very broad optical section system
> or are there other things to take into consideration?
>
> Many thanks
>
> Stuart
> --
> View this message in context: http://confocal-microscopy-list.588098.n2.nabble.com/Two-photon-imaging-with-low-NA-objectives-tp4979404p4979404.html
> Sent from the Confocal Microscopy List mailing list archive at Nabble.com.