Type F oil

[Gary Laevsky]

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Hi All,

I'm looking for a source for Type F oil, n=1.518 at 23c, dispersion=41, and
viscosity 410cst.

Cargille is my normal go to, but I don't see that they have it.

I did find;

Immersion Oil *Type LDF*

*Cat #:* 16241
*Refractive Index @ 23° C:*
F Line (486.1 nm) – 1.5239
e Line (546.1 nm) – 1.5181
D Line (589.3 nm) – 1.5150
C Line (656.3 nm) – 1.5115
*Viscosity:* 500 cSt ± 10% @ 23°C (Medium)

Assuming I do not mix and match, what do you think?

Thanks in advance.



--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Co-Founder, North Atlantic Microscopy Society (NAMS)
https://namsmicroscopy.com/
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

North Atlantic Microscopy Society Spring Symposium at The Rockefeller
University, April 24, 2019.

[McDonald, David L-2]

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Hi Gary,

Years ago we switched to Cargille Type HF, Cat #: 16245, which has worked very well for us, after they quit making Type DF.

IIRC they discontinued Type DF after losing the source of a main ingredient.

We also tried Type LDF at that time but found it to have a very obnoxious odor.

Best,
Dave

-------------------------------------------------------------
Dave McDonald
Fred Hutchinson Cancer Research Center
Scientific Imaging Lab, DE-512
1100 Fairview Avenue North
Seattle, WA 98109
206-667-4205
[hidden email]


-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Gary Laevsky
Sent: Thursday, April 18, 2019 6:25 AM
To: [hidden email]
Subject: Type F oil

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Hi All,

I'm looking for a source for Type F oil, n=1.518 at 23c, dispersion=41, and viscosity 410cst.

Cargille is my normal go to, but I don't see that they have it.

I did find;

Immersion Oil *Type LDF*

*Cat #:* 16241
*Refractive Index @ 23° C:*
F Line (486.1 nm) – 1.5239
e Line (546.1 nm) – 1.5181
D Line (589.3 nm) – 1.5150
C Line (656.3 nm) – 1.5115
*Viscosity:* 500 cSt ± 10% @ 23°C (Medium)

Assuming I do not mix and match, what do you think?

Thanks in advance.



--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Co-Founder, North Atlantic Microscopy Society (NAMS) https://urldefense.proofpoint.com/v2/url?u=https-3A__namsmicroscopy.com_&d=DwIFaQ&c=eRAMFD45gAfqt84VtBcfhQ&r=HnTG1v3GxyNuOwGPVn3A26XKW_94FyCfJe3qeEfd7-Q&m=Vj1qZ-0atGFk3W7kr-W5EQNzHbQJZEjQzl7k6x7MxR4&s=II16Ezr0Zgr1ke4JyTXTIHjJFREvvn-oDuTQeIarEeQ&e=
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

North Atlantic Microscopy Society Spring Symposium at The Rockefeller University, April 24, 2019.

[samjlord]

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For Nikon microscopes, I use Olympus F oil. I don't like the new Nikon F oil, because of its smell and that it dries to a hard lacquer. And Nikon N oil doesn't work with Perfect Focus. Cargille LDF smells terrible. Cargille HF is too autofluorescent for sensitive fluorescence microscopy. Olympus type F has been great for the last several months, and we plan to continue using that.

Here's a writeup of my testing of different oils:
https://blog.everydayscientist.com/?p=3652

[Steffen Dietzel]

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Whatever oil you decide on, may I recommend to check with your new
(non-manufacturer-recommended) oil for chromatic aberration? On a Leica
microscope when using Cargille HF we found quite a bit of it with 405 nm
exc. and it was also problematic for STED depletion at 770 nm. (The PSF
was looking just as good, btw. So no problem for monochromatic
applications. Or if you correct your z-depth by post-acquisition image
processing.)

Keep in mind that the oil with its assumed optical properties is part of
the manufacturer designed optical pathway, just like the 170 µm
coverslip. If you change the optical path, the built-in error-correction
of the objective may or may not still work.

Best

Steffen

Am 19.04.2019 um 18:45 schrieb Sam Lord:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> For Nikon microscopes, I use Olympus F oil. I don't like the new Nikon
> F oil, because of its smell and that it dries to a hard lacquer. And
> Nikon N oil doesn't work with Perfect Focus. Cargille LDF smells
> terrible. Cargille HF is too autofluorescent for sensitive
> fluorescence microscopy. Olympus type F has been great for the last
> several months, and we plan to continue using that.
>
> Here's a writeup of my testing of different oils:
> https://blog.everydayscientist.com/?p=3652
>

--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

http://www.bioimaging.bmc.med.uni-muenchen.de