*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
I attempted posting updates on the University of Arizona spectral database but they seemed to not have made it onto this list.
(I can not post directly through lists.umn.edu and there seems to be a delay after creating and validating a new account for this list)
The University of Arizona spectral database is back online at
http://spectra.arizona.edu .
It is now hosted on University IT equipment and passed security checks.
The display is based on Adobe Flash and therefore does not work on Operating Systems not supporting flash/swf. I looked into converting the application to html5 but that was not successful and therefore you most likely will need to use a desktop computers to access it.
The database contains spectra from many sources including our own Cary5 and Fluorolog, but also spectra I scanned using Un-Scanning software as George described in a follow-up post. The 2P spectra collection includes the ones from drbio @ Cornell but also spectra from publications from the Department of Physics at Montana State University and others.
Sometime this summer, I hope to receive keys for https connections which would also allow for remote spectra updates, hopefully through volunteers of this community.
Urs Utzinger
Biomedical Engineering, University of Arizona
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of David Johnston
Sent: Friday, May 2, 2014 8:59 AM
To:
[hidden email]
Subject: a tool to help create spectra for confocal databases
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
If you can't find the actual data values for a new dye, just a curve, I've just come across this very useful bit of freeware
http://sourceforge.net/projects/digitizer/files/Engauge%20Digitizer/digitizer-4.1/
you import an image file of the curve, define the axes, click on points on the curve and it'll spit out a .csv file of the data for you.
It is going to save me hours of manual work, aligning curves to a grid, reading off data points writing them down and then entering into excel and I thought that it might be useful to others as well as I don't recall seeing a post on this before.
Note, the latest version seems to be missing some crucial libraries and won't run on win7 64 bit, but version 4.1 (the link above) works fine.
No interest other than a very grateful user.
Regards,
Dave Johnston,
Bioimaging Unit, Southampton, UK
Locked
