UPDATE: What happened to this objective?

[Giang, William]

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Hi all,

I'm happy to report we were able to undo the damage.

It took 5-7 rounds of alternating applications of EtOH/water (5-10s exposure) followed by wiping with lens paper.

Before: https://imgur.com/p38eD22
After: https://imgur.com/a/WWCPxHH
In action: https://imgur.com/a/yfR5Oez

Thank you to everyone who provided suggestions for solvents/protocols as well as creative punishments.

Best,
Will


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Steven Nedellec
Sent: Friday, February 8, 2019 7:10 AM
To: [hidden email]
Subject: Re: What happened to this objective?

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Hi William

We encountered the same problem with all of our Nikon Oil Immersion objectives. Apparently, the new immersion oil provided by Nikon seems to be "aggressive" if not perfectly cleaned after use. Even our Nikon contact in France agreed with the fact that is oil may be very good optically but can be a little hazardous when used on a multi users device. Apparently they don't have any surrogate...

We tried to clean the best we could with water and then very little amount of acetone, but it's risky. And it does not work when there are too much spots.

If other people noticed the same problem with Nikon oil, it should be interesting to share on this topic.

best

Steven Nedellec

MicroPICell facility manager

Center Of Excellence Nikon

Nantes University, france

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