Unique opportunity: Leica Confocal TCS SPE - BEST OFFER

[Tanguy Lechertier]

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Hello,
We are a biotech company based in London (UK). Due to unforeseen circumstances, we are selling our beloved Leica confocal microscope. It is in perfect condition, equipped with 4 lasers, a climate chamber (Temperature and CO2) and an anti-vibration table. It is fully automated (xyzt) and can accommodate microplates, petri-dishes and microscope slides.
Since this is happening at short notice, make your best offer!
See below for the specs.
Feel free to contact me with any offer.

Buyer will pay for the relocation cost.


Description:

The TCS SPE confocal system comes as a kit consisting of confocal module, inverted stand, laser supply unit, SuperZ galvanometric positioning system and computer workstation. TCS SPE confocal module with SP detector The TCS (true confocal scanner) is a patented true confocal point scanning system. It represents the highly sensitive, filter-free spectral detection design with computer controlled adjustable bandwidth. The flexible laser incoupling allows the use of up to 4 lasers (3 visible and 405 nm).
The scan head includes the Leica patented SP detector for very high photon efficiency, allowing illumination at minimum laser intensity. Clear and contrast-rich images are recorded and the sample is protected from photodamage. The prism-based dispersion principle ensures waste-free fluorescence detection at all wavelengths and polarization orientations. With the freely tunable bandwidth of the spectrometer-detector (430 750nm with 5 nm spectral resolution), the emission collection can easily adapt to new dyes. Consequent sequential multiplexing results in optimal channel separation. A Lambda-Scan mode permits recording of unbiased spectral image series due to filter-free design. All channels receive the same signal intensity and are easily quantifiable.

Other scanner features are:
* True confocal point scanner for highest axial image resolution
* Adjustable speed 400, 600, 800 Hz (bidirectional mode doubles scan frequency)
* Adjustable scan formats 128, 256, 512, 1024, 2048 (pixels per edge)
* Max. frame rate 6.1 fps
* Field of view (FOV) 15.5 mm diagonal in the intermediate image plane
* Hardware zoom 1x 58x
* Support for optional bright field detector
* Square detection pinhole for maximal optical resolution (diameter 20-600 ?m)
* Automatic and user-friendly optimization of the pinhole diameter, manually adjustable for maintaining exact colocalization of multi-colour samples
* Multidimensional image acquisition in x, y, z (spatial dimensions) and t (time), lambda (wavelength) and combinations thereof
* Supports z-galvanometer for real-time cross-sections
* User friendly, intuitive LAS AF control software
* Sequential scanning for crosstalk free multi-color imaging (stack-wise, frame-wise)
* Digitization: 8 / 12 bit for PMT detection

Laser supply unit with beam splitters, tunable intensity modulation, beam merging and power supply Multi-Laser-Combination (Red Yellow Blue Violet) laser kit containing:
* Solid state lasers with 405 nm / 25 mW, 488nm / 10 mW, 561 nm / 20 mW, 635 nm / 18 mW
* Acousto Optical Tunable Filter (AOTF) for precise laser control (0-100%, continuously tunable)
* Autoranging power supply 100-240 V
* Beam Splitter Combinations 405/488/561/635 nm, 488/561 nm, Neutral splitter for TLD 30/70 %, Beam splitter control automated by software (manual override possible)

Inverted Research Microscope based on the DMi8 series prepared for confocal scanning with TCS SPEII.
Usability features:
* Automated focus drive (i.e. motorized and software-controlled)
* Automated objective revolver with 6 positions suitable for objectives 1.25x 100x
* Automated fluorescence turret with 6 positions for filter cubes
* Automated transmission light arm supports optional TLD photomultiplier
* Motorized side ports e.g. for switching to confocal scanning or camera switching
* Front panel with touch screen
* Side panel controlling IL/TL and apertures
* Side panel right hand with 4 configurable function keys
* Binocular tube "Ergo" (swivel-mounted)
* Fluorescence Intensity-Manager motorized

Available contrast methods:
* Brightfield
* Epi-Fluorescence

Illumination equipment:
* Lamp mount, straight for DMi8
* LED brightfield light source with TTL shutter
* Alignment-free light source EL 6000 (LQ-HXP 120 LEJ) with fast shutter and typical bulb lifetime of 2000 h
* Liquid light guide for thermal and mechanical insulation of light source from stand

Included accessories:
* Microscope controller CTR Advanced (supports motorized stages)
* Prepared for automated interference contrast (DIC), prisms and filters available separately
* Coded condensor base for S1/S28 lenses
* Condensor lens S28/0.55
* Eyepieces HC PLAN 10/20 BR/BR M (10x magnification and field size 20)
* Laser safety equipment
* Immersion oil type F

SuperZ galvanometric Z-drive for rapid focussing independent from objective
* Travel range 400 ?m
* Step size 42 nm
* Resolution 10 nm
* Optional inserts for glass slides, Petri dishes up to 35 mm or multiwell plates available

This optical outfit contains all necessary parts for SPEII systems based on DMi8 to do differential interference contrast (DIC) Contents:
* Automated DIC Prism turret with 4 positions
* Condenser head 0.53 / S23
* Automatic adjustment of Bias (fine tuning) of Wollaston prisms (incl. manual override by Software)
* Height adjustment and field diaphragm adjustment
* Adjustment of Koehler illumination
* Suitable for all magnifications 1.25x up to 100x objectives
* Filter system "Analyzer" for transmitted light DIC Note: This optical outfit works with DMi8 automated
* and DMi8 autofocus only.
* SPEII TLD Kit Inverted for DMi8 for digital recording of DIC or BF images along with confocal scanning

Climate Chamber Kit for SPEII systems based on DMi8
Kit contains:
* Pecon Incubator LSM 2000
* Pecon Heating Unit 2000
* Pecon TempController 20002
* Air Tube
* License for temperature control by software

Pecon Incubator LSM 2000
* Black chamber covering DMi8 microscope to be used with Leica TCS SPE
* 4 slidable front doors, 2 back doors for cable transfer
* Approx. dimensions (LxWxH): 630 x 550 x 380 mm

Pecon TempController 2000-2
2-independently controllable temperature channels (one is used by Heating Unit 2000)
Temperature can be set in steps of 0.1 °C
Display shows both channels (acutal) simultaneously
USB connector allows software control of temperature as well as recording of actual values

Pecon Heating Unit 2000
* Produces warm air and ventillation for Pecon Incubator
* Temperature set point-controlled trough Temp-Controller 2000-2

CO2 control set for SPE II climate chamber Available range for the CO2 setpoint value from 0.0 Vol-% up to 20.0 Vol-%: Resolution of the CO2 display: 0.1 Vol%

Humidifier to humidify the air in the Incubator P 2000, Incubator PM 2000, Incubator MMK 2000, Heatable Incubation Insert P-set 2000, Cooling/Temperable Incubation Insert P-set 2000 or any CO2-Covers.
This humidifier has a volume 250 ml and is connected to a CO2-Controller.

[Craig Brideau]

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To join, leave or search the confocal microscopy listserv, go to:
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A colleague has just started up a brand-new imaging lab in Reno, Nevada,
and is looking for a postdoc with 2P neuroscience in vivo imaging
experience. I am posting the ad here on her behalf (link to official job
posting at bottom):

The University of Nevada, Reno School of Medicine Department of Physiology
& Cell Biology is currently seeking a postdoctoral fellow to join a
neurovascular imaging laboratory. The applicant will have a deep
understanding of the neurovascular coupling and cerebral blood flow
regulation.

The successful applicant will be responsible for conducting independent
experiments that involve delicate surgeries, complex pharmacological
manipulations and highly technical imaging. The ideal candidate has
extensive knowledge of vascular biology and/or neuroscience. The incumbent
will be required to write manuscripts with guidance from the principal
investigator, and present data at national and international conferences.
The position requires great technical skills, ability to adapt,
troubleshoot experimental protocols, and some scientific creativity.
­­­­­­Some computer skills, including Matlab, are highly recommended.

Our lab utilizes two-photon fluorescent light microscope (TPFLM) to
understand how neural cells communicate with vascular cells in order to
regulate blood flow to match with metabolic demands. The unique strengths
of our approach include state-of-the art TPFLM, awake in vivo mouse model,
and advanced techniques including optogenetics.  In addition, we use
complementary in vitro model to probe different physiological parameters to
understand the interaction among members of the neurovascular unit. We are
also interested in uncovering how local signals are integrated to globally
regulate blood flow distribution to match moment-to-moment brain activity.

https://nshe.wd1.myworkdayjobs.com/en-US/UNR-external/job/Medicine-Reno---University-of-Nevada-School-of-Medicine/Postdoctoral-Scholar--Physiology_R0113002


Craig