Upgrading to Win 10

[George McNamara]

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build new computer (NVMe SSDs, lots of ram, etc), note static IP
addresses (label on back of card! also screen shot all adapter settings
in device manager etc) move over dual Ethernet card (and USB to
controller). Test new computer, if it works, great, if not, move card(s)
back. I do not think there is anything special about the dual 1Gbe card
Leica uses for their Z640 ... could probably just use any 1 Gbe (or
waste a 10 Gbe port) card with right static IP address. The dual card is
for SP8 scanhead + SMD (i.e. FALCON) data streams.

==> I like Steffen Dietzel's suggestion of (careful) BIOS update of the
HP Z640. I'll also consider doing this for our FV3000RS HP Z440.

***

Our SP8 has been too busy this week, so I don't know when I will add a
10 Gbe Ethernet card to its Z640 (after carefully re-documenting the
dual Ethernet card settings, including printouts). Already have CAT7
Ethernet cable to our Netgear 8-port 10Gbe (now $500) ... original box
purchased 2/2019 worked at only 1 Gbe once 4 cables attached - contacted
Nrtgear, replacement arrived next day (and defective unit shipped back
out on their fedex label). Other ports occupied by our file server,
FV3000RS PC, my office PC ... future -- I hope: activate the CAT7 to 10
gbe Transceiver port in I.T. closet (CAT7 cable, under 100') to spew
data directly to user's Microsoft OneDrive's - each JHU staff member and
student have 5 Tb allocation (and more can be added ... at ~1 Gigabyte
per hour on SP8 will be a while before anyone fills up their OneDrive
allocation from SP8 or FV3000RS use).

https://www.amazon.com/gp/product/B075Q66RKF

Other stuff I wrote about recently ...

https://www.amazon.com/gp/product/B07MVGGG3Z

GLOTRENDS 4-Bay M.2 NVME Adapter, Soft RAID Support, Up to PCIE 3.0 X8
Bandwidth

==> installed fine, 1.0 Gigabytes/sec copy/paste Windows 10. No need for
bifurcation (unlike Hyper M.2 card below).

https://www.amazon.com/ASUS-M-2-X16-V2-Threadripper/dp/B07NQBQB6Z

ASUS Hyper M.2 X16 PCIe 3.0 X4 Expansion Card V2 Supports 4 NVMe M.2

==> requires BIOS bifurcation (HP Z640 and Z440 do ... have Hyper M.2 in
each of SP8 and FV3000RS PCs), 1.0 Gigabytes/sec copy/paste Windows
10.One PC (7 years old???) did not recognize the PCIe card (and BIOS
lacked bifurcation anyway), whereas FGlotrends card worked.


This QNAP card, dual 10 Gbe Ethernet plus two NVMe (not included) also
looks interesting

https://www.amazon.com/QNAP-QM2-2S-220A-Low-Profile-Pre-Loaded-Full-Height/dp/B0711NWRPW


On 1/28/2020 7:55 AM, Arvydas Matiukas wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Steffen,
>
> Thanks a lot. This is a great recourse for do-it-yourself enthusiasts.
>
> I wonder if you have a procedure to move/transfer confocal software
> to another/new computer in case old computer (i.e. motherboard) dies.
>
> More generally what precaution measures do you recommend to deal with
> the situation (less likely then disk damage/death but still not
> impossible after
>> 4-5 years)
> Thanks,
> Arvydas
>
>
>
>
>
> +++++++++++++++++++++++++++++
> Arvydas Matiukas, Ph.D.
> Manager of NRB Shared Equipment
> Director of Confocal&Two-Photon Core
> SUNY Upstate Medical University
> Neuroscience & Physiology Dept
>
>
> Syracuse, NY 13210
>
>
>>>> Steffen Dietzel <[hidden email]> 01/28/20 7:15 AM >>>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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>
> Post images on
> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=Eij_pLUIOG-byyODG-K6bT9hbHbcwNsdQKZnHSDBtW8&e=
>   and include the link in your posting.
> *****
>
> Dear all,
>
> coming back to this thread from November,  I can now report that in the
> meantime I successfully updated eight Leica microscope computers from
> Windows 7 to Windows 10 (Enterprise long term support), including three
> SP8 systems.
>
> While the road was bumpy, they now all run fine without problems. Once I
>
> figured out how to do it, it was rather simple for the following
> comparable systems (WF or SP8). Still time consuming though. The key to
> sleeping well is to make a backup of the system disk before starting
> anything, no surprise here.
>
> Because I had that many systems to upgrade, I made a web page with the
> procedure that I used as reference with the following systems. Since it
> might be useful to some of you, here is the link.
> https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_manuals-2Dprotocols_maintenance-5Fprotocolls_win7towin10_index.html&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=5lafTeoK-FTvGr-LVudmzLXQy3JJHEG29aWxam7JMMk&e=
>
>
> This is not a procedure endorsed by Leica, but it worked for us. It
> would be of interest to me if this is actually helpful to anybody, if so
>
> I would appreciate a message. (I won't be able to read e-mails from this
>
> account next week and the week thereafter, sorry, any reply will be
> delayed).
>
> Thanks to those who helped with valuable tips.
>
> The usual disclaimer applies: No warranties. Enter at your own risk.
> Make a backup first.
>
> Best
>
> Steffen
>
>
>

[Ann-W]

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Hi Aryydas

What I'd say, in our experience of this, is you need to be very careful about if the new motherboard will support the peripheral devices the confocal needs. Quite a lot of microscopy peripherals still require a serial port. Our experience of this was that not all modern motherboards are capable of driving serial devices - or indeed have sufficient ports for this. We take advice from our IT department on this, but we are fortunate to have a proactive IT department.

We also noted that not all serial cards are alike, some of them are fake so when trying to reimplement a confocal - or indeed anything else complex on a new PC with several peripherals you can end up going through several serial cards.

Wrt reinstalling software, in the good old days you got a DVD with the software on. We have taken the precaution of putting the software onto our facility cloud data-storage solution. Some software still use dongles which makes life easy, others you need to validate the MAC address of the new computer with the company. I guess then it depends on your relationship with the company.

Hope this helps

Ann


-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Arvydas Matiukas
Sent: 28 January 2020 12:56
To: [hidden email]
Subject: Re: [EXTERNAL] Re: Upgrading to Win 10 (Leica)

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Hi Steffen,

Thanks a lot. This is a great recourse for do-it-yourself enthusiasts.

I wonder if you have a procedure to move/transfer confocal software to another/new computer in case old computer (i.e. motherboard) dies.

More generally what precaution measures do you recommend to deal with the situation (less likely then disk damage/death but still not impossible after
>4-5 years)

Thanks,
Arvydas





+++++++++++++++++++++++++++++
Arvydas Matiukas, Ph.D.
Manager of NRB Shared Equipment
Director of Confocal&Two-Photon Core
SUNY Upstate Medical University
Neuroscience & Physiology Dept


Syracuse, NY 13210


>>> Steffen Dietzel <[hidden email]> 01/28/20 7:15 AM >>>
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Post images on
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=Eij_pLUIOG-byyODG-K6bT9hbHbcwNsdQKZnHSDBtW8&e=
 and include the link in your posting.
*****

Dear all,

coming back to this thread from November,  I can now report that in the meantime I successfully updated eight Leica microscope computers from Windows 7 to Windows 10 (Enterprise long term support), including three
SP8 systems.

While the road was bumpy, they now all run fine without problems. Once I

figured out how to do it, it was rather simple for the following comparable systems (WF or SP8). Still time consuming though. The key to sleeping well is to make a backup of the system disk before starting anything, no surprise here.

Because I had that many systems to upgrade, I made a web page with the procedure that I used as reference with the following systems. Since it might be useful to some of you, here is the link.
https://urldefense.proofpoint.com/v2/url?u=https-3A__www.bioimaging.bmc.med.uni-2Dmuenchen.de_manuals-2Dprotocols_maintenance-5Fprotocolls_win7towin10_index.html&d=DwIDaQ&c=ogn2iPkgF7TkVSicOVBfKg&r=DtXPX1Vw9uh8rqlCEC9BTBr2oYBP4dEG1ecsgK6l-4k&m=ioytTPC1TM5UKhc046oaNOiCeg2gXbRKTMkbongtDE0&s=5lafTeoK-FTvGr-LVudmzLXQy3JJHEG29aWxam7JMMk&e=


This is not a procedure endorsed by Leica, but it worked for us. It would be of interest to me if this is actually helpful to anybody, if so

I would appreciate a message. (I won't be able to read e-mails from this

account next week and the week thereafter, sorry, any reply will be delayed).

Thanks to those who helped with valuable tips.

The usual disclaimer applies: No warranties. Enter at your own risk.
Make a backup first.

Best

Steffen



--
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Biomedical Center (BMC)
Head of the Core Facility Bioimaging

Großhaderner Straße 9
D-82152 Planegg-Martinsried
Germany

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