Dear Shiv
Is your Verdi is 10W? If you have 10W pump power you should be able
to auto-tune it easily in the range of 720-980nm. Yes, it is true that it may
be noisy about 20nm on both ends for 10W pumping. If it’s a manual tuning
and if you are an expert, you have lot of options to make the laser stable and
mode locked.
I think we discussed in the email server before, most of the red
fluorophores works pretty good between 740-760nm. We are using 740 for mCherry,
mOrange, mKate, mKO and tdTomato, provide very good signal with our Biorad
Radiance2100 with Nikon 60xIR lens. We use only few microwatts average power at
the specimen plane. Of course double the wavelength will be nice.
Hope this helps
ammasi
From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Mayandi
Sivaguru
Sent: Friday, October 17, 2008 3:53 PM
To: [hidden email]
Subject: Usefulness of 1040-1080 nm in 2P Excitation
Hi all, I have seen articles using 1030 nm Ex for mCherry, but the Coherent site
showing data that it is best excited with 1060 nm, how significant the range
1040-1080 nm apart from mCherry in your experience for any other probes. In my
personal experience, attempts to tune the laser closer to low and high end of
the tuning range (730-930nm, particularly trying to obtain an excitation
profile), in a previous version of Chameleon often tend to send the laser out
of mode lock and requires a 15-30 min PZT recovery.
Thanking you in advance for your insights in this regard
Shiv
Ammasi Periasamy, Ph.D.
Director, Keck Center for
Cellular Imaging (KCCI)
Professor of Biology and
Biomedical Engineering
Biology, Gilmer Hall (064),
McCormick Rd
University of Virginia
Charlottesville, VA 22904
Voice: 434-243-7602 (Office);
982-4869 (lab)
Fax:434-982-5210; Email:[hidden email]
http//:www.kcci.virginia.edu
************************
Workshop on FRET Microscopy,
March 3-7, 2009
http://www.kcci.virginia.edu/workshop/workshop2009/index.php
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