Uvitex 2b / BBOT / Fluorescent Brightener 184

[Kubow, Kristopher E - kubowke]

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I'd like to know if anyone has a protocol for preparing and using the stain Uvitex (also known by many other names: http://www.chemicalbook.com/CASEN_7128-64-5.htm). I have a researcher who ordered it to stain crayfish intestines. However it came as a solid powder rather than a solution, and without any instructions. I've never heard of the stain before and there is very little obvious information out on the internet. Based on its chemical structure, I'm thinking that they need to make a concentrated stock solution in a non-polar solvent such as DMF or DMSO. But I'd like to know if anyone has a standard protocol for this.

Thank you,

Kristopher Kubow
Assistant Professor
Director of the Light Microscopy Facility
Department of Biology
James Madison University
Harrisonburg, VA 22807
[phone]
Bioscience 2036
http://csmbio.csm.jmu.edu/biology/microscopy/

[Martin Wessendorf-2]

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If you don't get an answer here, you might want to check with the
histonet listserv--that's also a very active group:
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Good luck!

Martin Wessendorf



On 4/9/2015 11:15 AM, Kubow, Kristopher E - kubowke wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> I'd like to know if anyone has a protocol for preparing and using the stain Uvitex (also known by many other names: http://www.chemicalbook.com/CASEN_7128-64-5.htm). I have a researcher who ordered it to stain crayfish intestines. However it came as a solid powder rather than a solution, and without any instructions. I've never heard of the stain before and there is very little obvious information out on the internet. Based on its chemical structure, I'm thinking that they need to make a concentrated stock solution in a non-polar solvent such as DMF or DMSO. But I'd like to know if anyone has a standard protocol for this.
>
> Thank you,
>
> Kristopher Kubow
> Assistant Professor
> Director of the Light Microscopy Facility
> Department of Biology
> James Madison University
> Harrisonburg, VA 22807
> [phone]
> Bioscience 2036
> http://csmbio.csm.jmu.edu/biology/microscopy/

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
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6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
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[Rosemary.White]

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Hi Kristopher,

We use Uvitex to stain plant cell walls that stain poorly with calcofluor
white/tinopal. It's been used a bit to stain fungal cell walls, e.g.
Wachsmuth, E. D. 1988. Visualization of fungi in histological sections.
Virchows Arch. B Cell Pathol. 56:1­4.

There is a whole range of these fluorescent brighteners, often with very
similar names. If you search for "fluorescent brightener" you'll see what
I mean.

Some are water-soluble, some are not, and some are more soluble in a
slightly acid or alkaline solution.

We make a stock solution of Uvitex 2B in DMSO then dilute this to make a
working solution of 0.1-0.001% in water. It's very bright.

cheers,
Rosemary

Dr Rosemary White
CSIRO Black Mountain
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
E [hidden email]


On 10/04/15 2:15 AM, "Kubow, Kristopher E - kubowke" <[hidden email]>
wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>Post images on http://www.imgur.com and include the link in your posting.
>*****
>
>I'd like to know if anyone has a protocol for preparing and using the
>stain Uvitex (also known by many other names:
>http://www.chemicalbook.com/CASEN_7128-64-5.htm). I have a researcher who
>ordered it to stain crayfish intestines. However it came as a solid
>powder rather than a solution, and without any instructions. I've never
>heard of the stain before and there is very little obvious information
>out on the internet. Based on its chemical structure, I'm thinking that
>they need to make a concentrated stock solution in a non-polar solvent
>such as DMF or DMSO. But I'd like to know if anyone has a standard
>protocol for this.
>
>Thank you,
>
>Kristopher Kubow
>Assistant Professor
>Director of the Light Microscopy Facility
>Department of Biology
>James Madison University
>Harrisonburg, VA 22807
>[phone]
>Bioscience 2036
>http://csmbio.csm.jmu.edu/biology/microscopy/