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Hi Leandro,
Seems like there is quite a literature on hemin fluorescence and detection.
Something like this might be adaptable for microscopy:
A simple and sensitive fluorescent assay for hemin detection based on artemisinin-thiamine
https://doi.org/10.1016/j.snb.2018.06.052Good luck!
Michael
On 16/02/2021 17:46, Leandro Lemgruber wrote:
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Hi list
a user in our facility wants to visualise which cells take up/bind Hemin. The available Hemin antibody isn't working well, so we wonder if we could directly visualise hemin - we think it would emit some fluorescence itself.
My question is: has anyone done such a thing? And if yes, would be willing to share your set-up and expertise?
All the best
Leandro
--
[Jockey Club College of Veterinary Medicine and Life Sciences - City University of Hong Kong]
Dr. Michael Doube
BVSc, BPhil, PhD, PGCert Vet Ed, MRCVS, FRMS
Associate Professor
Department of Infectious Diseases
and Public Health
5/F, Block 2, To Yuen Building
31 To Yuen Street
Kowloon, Hong Kong
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