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White light lasers are continuum generators, so must spread their energy
over their entire spectrum. They are particularly weak in the blue so they
are frequently accompanied by a separate blue or violet diode laser for
higher-power applications. For FRAP >40 mW out of the laser is usually
sufficient unless your optical system is very lossy. This is a typical
number from my own experience, and will vary depending on your microscope
and fluorophore, as well as environmental conditions such as oxygen in the
medium and other photochemical factors.
Craig
On Sat, Apr 11, 2020 at 2:36 PM Konstantin <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
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> *****
>
> Dear colleagues
> If the power for WLL is not sufficient for FRAP experiments what power for
> additional solid state laser (for example, for 488 nm) would you recommend?
> Patience and Safety for all.
> Best regards,
> Konstantín
>
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