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Broadcast date: Thursday, November 1, 11 am ET
Length: Approximately one hour
Speaker: Hari Shroff, Ph.D., National Institute of Biomedical Imaging and
Bioengineering (NIBIB)
Moderator: Barbara G. Goode, Editor in Chief, BioOptics World
Thanks to the combination of high speed and low
photodamage/photobleaching they provide, light sheet-based fluorescence
microscopy (LSFM) techniques are gaining popularity in live-cell imaging. But
while the power of these techniques is impressive, the biological community
has been slow to adopt them, for two reasons: Their relatively low axial
resolution leads to resolution anisotropy, and it is difficult to implement the
necessary orthogonal geometry between excitation and detection.
This webcast will explain efforts to deal with both problems, and the
new technology that has emerged as a result: Inverted selective plane
illumination microscopy (iSPIM). iSPIM replaces the brightfield illumination pillar
of a conventional microscope with a module that enables rapid volumetric
imaging of cells and embryos via plane illumination.
The presentation will discuss the potential of iSPIM for high speed,
noninvasive interrogation of nematode embryos. It will also describe the latest
attempts to increase resolution isotropy. The broadcast will conclude with an
open question-and-answer session.
What you will learn:
• What advantages light sheet-based fluorescence microscopy offers to life
scientists
• What efforts have been made to improve overcome speed and resolution
limitations
• How inverted selective plane illumination microscopy (iSPIM) works
• What are the advantages and limitations of iSPIM
Who should attend:
• Anyone interested in live-cell imaging
• Scientists and engineers wanting details on microscopy advances
• Designers of optical microscopy components or systems
• Cell biologists, biophysicists, biomedical researchers, bioengineers, and
other life scientists
About the speaker:
Hari Shroff, Ph.D. is a Principal Investigator in the National Institutes of Health
Intramural Research Program (NIH IRP) at the National Institute of Biomedical
Imaging and Bioengineering (NIBIB). He received a B.S.E. in bioengineering
from the University of Washington in 2001, and under the supervision of Dr.
Jan Liphardt, completed his Ph.D. in biophysics at the Unversity of California at
Berkeley in 2006. He spent the next three years performing postdoctoral
research under the mentorship of Eric Betzig at the Howard Hughes Medical
Institute's Janelia Farm Research Campus where his research focused on
development of photactivated localization microscopy (PALM). Dr. Shroff is
now chief of NIBIB's section on high resolution optical imaging laboratory,
where he and his staff are developing new imaging tools for application in
biological and clinical research.
To register sign up at:
http://www.asiimaging.com/fast-high-axial-resolution-3d-live-cell-imaging-with-plane-illumination-microscopy/
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