You are invited to
attend a live, interactive, web-based instructional seminar:
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"Quantitative Image and Data Acquisition for Fluorescent
Specimens"
Advice from a Facility Director
Presented by Brian Matsumoto, Ph.D., University of California, Santa
Barbara
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Details are
below. Connection lines are limited, so reserve yours now. There is no charge to
participate in this on-line seminar.
When:
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Thursday, 13-September, 1:00PM (Eastern time; 10 AM Pacific time.)
Duration: Approximately 1
hour.
Pre-register (required) at:
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href="blocked::https://mediacy.webex.com/mediacy/j.php?ED=99592442&RG=1"
target=_blank>https://mediacy.webex.com/mediacy/j.php?ED=99592442&RG=1
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wrapped, please re-build it in your web browser's address bar. The line begins
with "https" and ends with "RG=1"
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Details:
=====================
Attendees will
learn about issues affecting the quantitative accuracy of fluorescence images acquired through a
microscope and will pick up tips and suggestions for improving image quality, making better use of the
camera's light collection abilities, and will learn the nomenclature associated
with digital imaging. Attendees will leave with a better understanding of how to
characterize and optimize their optical system, camera, and software. Bring your questions to this live, interactive
web-based seminar.
- What is bit depth and when/why does it
matter?
- What is dynamic
range?
- Characterizing your camera's linear
range.
- Noise and other sources of data
uncertainty.
- Maximizing
your camera's light collection capabilities.
- Setting the
best exposures.
- Correcting for photobleaching.
- Collecting
images in 3D.
About the
presenter
=====================
Brian Matsumoto,
Ph.D. is the Director of the Integrated Microscopy Facility and is an
Associate Adjunct Professor for the department of Molecular, Cellular and
Developmental Biology Department at the University of California Santa
Barbara. He is the author of Basic Methods in Light Microscopy
(Cambridge University
Press) and editor of Cell Biological Applications of
Confocal Microscopy (Academic Press).
Upcoming Webinar: "Deconvolving 3D Fluorescence Images for
Quantitative Analysis" (invitations will be sent
separately).
Sponsored by
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href="blocked::http://www.mediacy.com/">Media Cybernetics (Image Pro and AutoQuant software) and <A
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href="blocked::http://www.qimaging.com/">QImaging (precision CCD
cameras)
This seminar requires
that attendees use a Java-enabled browser with a high bandwidth connection. Audio is via toll-free
telephone.
There is no charge to participate in this on-line
seminar.
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