What happened to this objective?

[Giang, William]

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Hi Confocal listers (and possibly readers of Microscopy Today),


I'm not sure what happened to our Nikon 60x TIRF objective. It reminds me of plastic wrap on new electronics when there's air stuck underneath.

**possible lens gore / not safe for microscopists warning ** See https://imgur.com/p38eD22 for a picture taken with my cellphone.

One of my coworkers suspects it's the result of uncured mounting media.

The typical optical lens cleaners have been ineffective.

Has anyone been unfortunate enough to see something similar?


Thanks,
Will



William Giang  |  Research Specialist
Whitehead Building, Room 465
615 Michael Street, Atlanta, GA 30322

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[Rowlands, Christopher J]

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I've seen something similar from a lab where someone had left Mount-Quick to dry on the objective. Like you, I went through all the 'safe' solvents, and then got increasingly desperate, and after I figured out it was Mount-Quick, I tried xylene (listed as the solvent on the Mount-Quick box) which did the trick. I did the usual cleaning procedure of folding a lens tissue in hemostats, dropping a few drops of xylene on the tissue edge and wiping once, followed by a gentle dab with another lens tissue to minimize the time xylene is eating away at the lens adhesives. Repeat until the lens is clean (it should be fairly obvious if it's working because the solid crusty stuff will start to come away, and you can see the interference fringes as it gets thinner).

I should point out my solution is absolutely not approved procedure, but I did it because the alternative was to send the objective back to the manufacturer, and at that point I thought I might as well risk the additional damage. So consider yourself warned - only to be tried once you're pretty certain the whole front element will have to be replaced anyway!

Chris

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Giang, William
Sent: 06 February 2019 21:33
To: [hidden email]
Subject: What happened to this objective?

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Hi Confocal listers (and possibly readers of Microscopy Today),


I'm not sure what happened to our Nikon 60x TIRF objective. It reminds me of plastic wrap on new electronics when there's air stuck underneath.

**possible lens gore / not safe for microscopists warning ** See https://imgur.com/p38eD22 for a picture taken with my cellphone.

One of my coworkers suspects it's the result of uncured mounting media.

The typical optical lens cleaners have been ineffective.

Has anyone been unfortunate enough to see something similar?


Thanks,
Will



William Giang  |  Research Specialist
Whitehead Building, Room 465
615 Michael Street, Atlanta, GA 30322

[hidden email]<mailto:[hidden email]>
ici.emory.edu<http://www.cores.emory.edu/ici/>


[http://www.cores.emory.edu/ici/images/EU_ICIC_EICF_SmlLogo.png]<http://www.cores.emory.edu/ici/>



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This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited.

If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).

[Sylvie Le Guyader]

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Have you tried water? One tends to forget but it actually removed a fair number of stuff.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7C,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website
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-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Giang, William
Sent: den 6 februari 2019 22:33
To: [hidden email]
Subject: What happened to this objective?

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Hi Confocal listers (and possibly readers of Microscopy Today),


I'm not sure what happened to our Nikon 60x TIRF objective. It reminds me of plastic wrap on new electronics when there's air stuck underneath.

**possible lens gore / not safe for microscopists warning ** See https://imgur.com/p38eD22 for a picture taken with my cellphone.

One of my coworkers suspects it's the result of uncured mounting media.

The typical optical lens cleaners have been ineffective.

Has anyone been unfortunate enough to see something similar?


Thanks,
Will



William Giang  |  Research Specialist
Whitehead Building, Room 465
615 Michael Street, Atlanta, GA 30322

[hidden email]<mailto:[hidden email]>
ici.emory.edu<http://www.cores.emory.edu/ici/>


[http://www.cores.emory.edu/ici/images/EU_ICIC_EICF_SmlLogo.png]<http://www.cores.emory.edu/ici/>



________________________________

This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited.

If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments).


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[Dan Metcalf-2]

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If it is hardened immersion oil place a drop of the same immersion oil on the contaminated objective, leave it for a few minutes and then gently wipe it off with lens tissue wrapped around a cuticle stick. Finish the cleaning with ethanol.

Regards,

Dan Metcalf

(Nikon UK)

[Steven Nedellec]

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Hi William

We encountered the same problem with all of our Nikon Oil Immersion objectives. Apparently, the new immersion oil provided by Nikon seems to be "aggressive" if not perfectly cleaned after use. Even our Nikon contact in France agreed with the fact that is oil may be very good optically but can be a little hazardous when used on a multi users device. Apparently they don't have any surrogate...

We tried to clean the best we could with water and then very little amount of acetone, but it's risky. And it does not work when there are too much spots.

If other people noticed the same problem with Nikon oil, it should be interesting to share on this topic.

best

Steven Nedellec

MicroPICell facility manager

Center Of Excellence Nikon

Nantes University, france

[Seamus Holden-2]

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Hi Steven

Could you advise precisely which letter this new Nikon oil is (ie Type-?) so that we know to be careful of it!

Many thanks
Seamus


Dr Seamus Holden
Wellcome Trust Sir Henry Dale Fellow
Newcastle University
Centre for Bacterial Cell Biology
NE2 4AX, United Kingdom

Phone: [phone]
https://blogs.ncl.ac.uk/seamusholden/

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Steven Nedellec
Sent: 08 February 2019 12:10
To: [hidden email]
Subject: Re: What happened to this objective?

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Hi William

We encountered the same problem with all of our Nikon Oil Immersion objectives. Apparently, the new immersion oil provided by Nikon seems to be "aggressive" if not perfectly cleaned after use. Even our Nikon contact in France agreed with the fact that is oil may be very good optically but can be a little hazardous when used on a multi users device. Apparently they don't have any surrogate...

We tried to clean the best we could with water and then very little amount of acetone, but it's risky. And it does not work when there are too much spots.

If other people noticed the same problem with Nikon oil, it should be interesting to share on this topic.

best

Steven Nedellec

MicroPICell facility manager

Center Of Excellence Nikon

Nantes University, france

[Sylvie Le Guyader]

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Hi Steven

Nikon has several types of immersion oils. We have been using 2 of them in the past 10 years. We have 6 Nikon systems, all of which have at least one oil objectives and all of which are used an average of 4-6h/day. Also most of our systems at at 37 degrees all the time. We have never encountered that problem.

We have as a routine that each user cleans the used oil objectives with 100% ethanol after their session. As I understood this is not optimal because the oil and ethanol do not have the same polarity (or something like that) so the oil is not completely removed. Despite this we have not experienced any problem. We inspect our objectives regularly and find no crystals that would come from dry oil.

The only problem we sometimes have is that users can accidentally put oil on an air objective in which case we use petroleum to remove the oil then water to remove any small residue left behind if any.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7C,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website

________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Steven Nedellec [[hidden email]]
Sent: 08 February 2019 13:09
To: [hidden email]
Subject: Re: What happened to this objective?

*****
To join, leave or search the confocal microscopy listserv, go to:
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Post images on http://www.imgur.com and include the link in your posting.
*****

Hi William

We encountered the same problem with all of our Nikon Oil Immersion objectives. Apparently, the new immersion oil provided by Nikon seems to be "aggressive" if not perfectly cleaned after use. Even our Nikon contact in France agreed with the fact that is oil may be very good optically but can be a little hazardous when used on a multi users device. Apparently they don't have any surrogate...

We tried to clean the best we could with water and then very little amount of acetone, but it's risky. And it does not work when there are too much spots.

If other people noticed the same problem with Nikon oil, it should be interesting to share on this topic.

best

Steven Nedellec

MicroPICell facility manager

Center Of Excellence Nikon

Nantes University, france


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