What products do you use to glue a glass coverslip to a chamber?

classic Classic list List threaded Threaded
6 messages Options
Svetlana Kurilova Svetlana Kurilova
Reply | Threaded
Open this post in threaded view
|

What products do you use to glue a glass coverslip to a chamber?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi all,
we are using SYLGARD 184 silicone elastomer kit for gluing plates and
chambers. But I'm wondering are there any other alternatives you could suggest?

Sincerely,
Svetlana
Craig Brideau Craig Brideau
Reply | Threaded
Open this post in threaded view
|

Re: What products do you use to glue a glass coverslip to a chamber?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I actually use Sylgard for some of my stranger samples.  If I might ask,
why are you looking for alternatives?

Craig



On Fri, Mar 21, 2014 at 3:50 PM, Svetlana Kurilova <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi all,
> we are using SYLGARD 184 silicone elastomer kit for gluing plates and
> chambers. But I'm wondering are there any other alternatives you could
> suggest?
>
> Sincerely,
> Svetlana
>
mmodel mmodel
Reply | Threaded
Open this post in threaded view
|

Re: What products do you use to glue a glass coverslip to a chamber?

In reply to this post by Svetlana Kurilova
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Glue for fish tanks also works well, and it's much faster

________________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Svetlana Kurilova <[hidden email]>
Sent: Friday, March 21, 2014 5:50 PM
To: [hidden email]
Subject: What products do you use to glue a glass coverslip to a chamber?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi all,
we are using SYLGARD 184 silicone elastomer kit for gluing plates and
chambers. But I'm wondering are there any other alternatives you could suggest?

Sincerely,
Svetlana
Svetlana Kurilova Svetlana Kurilova
Reply | Threaded
Open this post in threaded view
|

Re: What products do you use to glue a glass coverslip to a chamber?

In reply to this post by Svetlana Kurilova
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Michael,
that product works just fine and I personally like it but:
1. there is never enough a curing agent for a provided amount of a base
agent (even considering the 1:9 mass ratio)
2. it takes more than 24 hours for clue to completely dry out
3. the shipping from that company is almost the same with a product price
mmodel mmodel
Reply | Threaded
Open this post in threaded view
|

Re: What products do you use to glue a glass coverslip to a chamber?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

That's why I suggest, as an alternative, the glue that you can buy in pet stores. Nontoxic and dries fast.
________________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Svetlana Kurilova <[hidden email]>
Sent: Saturday, March 22, 2014 3:49 PM
To: [hidden email]
Subject: Re: What products do you use to glue a glass coverslip to a chamber?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Michael,
that product works just fine and I personally like it but:
1. there is never enough a curing agent for a provided amount of a base
agent (even considering the 1:9 mass ratio)
2. it takes more than 24 hours for clue to completely dry out
3. the shipping from that company is almost the same with a product price
Marcus Knopp Marcus Knopp
Reply | Threaded
Open this post in threaded view
|

AW: What products do you use to glue a glass coverslip to a chamber?

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I have used duplication silicone for that purpose (picodent twinsil). Since it is used for humans, I supposed it to be non-toxic. It dries fast and is easy to remove without remainings.

Marcus