Caroline Bass <
[hidden email]> writes:
> Hello Everyone,
>
> I’m at the starting point for a new transgenic approach (viral vector
> mediated) and so it’s a good point to rethink the standard EGFP marker
> gene that I use. I know there are humanized GFP variants, mCherry,
> etc. Can anyone suggest a good marker for in vivo work? I will mostly
> use these vectors in the brain, and the FP will be used to image the
> spread of the virus. I want something bright, NON-toxic, and easy to
> work with. I can easily view red and green fluorescence, so something
> in these ranges are nice.
Well EGFP is already recodoned for mammalian expression systems. In
general red stuff is better in terms of less auto-fluorescent
background, while green/yellow is brighter and cameras are more
sensitive.
In my experience mCherry is the best red FP I have used, although someone
mentioned TagRFP to me a few days ago. I haven't yet had time to go
check the literature, or try it out. Anyone else have any experience with
TagRFP?
Ian
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