Who has purchased a fluorescence nanoscope?

[George McNamara]

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Dear Confocal listserv,

Who has purchased a fluorescence nanoscope? What can you tell me about
your experiences - either here or privately?

I see 11 OMX labs listed at http://www.api.com/omx-labs.asp (may be out
of date or some customers may be shy).

I am aware of STED systems in the USA at Yale Univ, NIH and I was told
San Diego (have not found where), and possibly UC Denver. Paul French
apparently did his own upgrade of a Leica SP2
(http://www.focusonmicroscopy.org/2008/PDF/070_Auksorius.pdf and a Leica
Scientific Forum video ... see also related work at
ftp://ftp.espci.fr/incoming/Gilles/INSERM-STED-x6.pdf ).

I am also aware of one 4pi microscope in the USA.

So, who has N-SIM, N-STORM, SR-SIM, PALm, SR-200, and what can you tell
me about your experiences with your (purchased) nanoscope(s)?

Sincerely,

George
p.s. Please no need to clutter up the listserv with other people's
nanoscope references - I know how to use PubMed. For that matter, I've
replicated the results of the following two papers on my confocal's:

    Subdiffraction fluorescence imaging of biomolecular structure and
    distributions with quantum dots. </pubmed/20600360>

    Heidbreder M, Endesfelder U, van de Linde S, Hennig S, Widera D,
    Kaltschmidt B, Kaltschmidt C, Heilemann M.

    Biochim Biophys Acta. 2010 Oct;1803(10):1224-9. Epub 2010 Jun
    23.PMID: 20600360


    Quantum dot triexciton imaging with three-dimensional subdiffraction
    resolution. </pubmed/19453186>

    Hennig S, van de Linde S, Heilemann M, Sauer M.

    Nano Lett. 2009 Jun;9(6):2466-70.PMID: 19453186

I have used <1 Airy unit pinhole to improve triexciton resolution
further. I have not tried doing 3D deconvolution on the data.


--


George McNamara, PhD
Analytical Imaging Core Facility
University of Miami

[G. Esteban Fernandez]

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UCLA has a STED

http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791
<http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791>
http://www.cnsi.ucla.edu/staticpages/core-facilities#alms


<http://www.cnsi.ucla.edu/staticpages/core-facilities#alms>

On Sat, Jan 29, 2011 at 7:32 AM, George McNamara
<[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Confocal listserv,
>
> Who has purchased a fluorescence nanoscope? What can you tell me about your
> experiences - either here or privately?
>
> I see 11 OMX labs listed at http://www.api.com/omx-labs.asp (may be out of
> date or some customers may be shy).
>
> I am aware of STED systems in the USA at Yale Univ, NIH and I was told San
> Diego (have not found where), and possibly UC Denver. Paul French apparently
> did his own upgrade of a Leica SP2 (
> http://www.focusonmicroscopy.org/2008/PDF/070_Auksorius.pdf and a Leica
> Scientific Forum video ... see also related work at
> ftp://ftp.espci.fr/incoming/Gilles/INSERM-STED-x6.pdf ).
>
> I am also aware of one 4pi microscope in the USA.
>
> So, who has N-SIM, N-STORM, SR-SIM, PALm, SR-200, and what can you tell me
> about your experiences with your (purchased) nanoscope(s)?
>
> Sincerely,
>
> George
> p.s. Please no need to clutter up the listserv with other people's
> nanoscope references - I know how to use PubMed. For that matter, I've
> replicated the results of the following two papers on my confocal's:
>
>   Subdiffraction fluorescence imaging of biomolecular structure and
>   distributions with quantum dots. </pubmed/20600360>
>
>   Heidbreder M, Endesfelder U, van de Linde S, Hennig S, Widera D,
>   Kaltschmidt B, Kaltschmidt C, Heilemann M.
>
>   Biochim Biophys Acta. 2010 Oct;1803(10):1224-9. Epub 2010 Jun
>   23.PMID: 20600360
>
>
>   Quantum dot triexciton imaging with three-dimensional subdiffraction
>   resolution. </pubmed/19453186>
>
>   Hennig S, van de Linde S, Heilemann M, Sauer M.
>
>   Nano Lett. 2009 Jun;9(6):2466-70.PMID: 19453186
>
> I have used <1 Airy unit pinhole to improve triexciton resolution further.
> I have not tried doing 3D deconvolution on the data.
>
>
> --
>
>
> George McNamara, PhD
> Analytical Imaging Core Facility
> University of Miami
>

[lechristophe]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

The Bordeaux Imaging Center in Bordeaux, France has a STED and a TIRF/PALM
http://www.bic.u-bordeaux2.fr/

PALM/STORM setups are becoming more common (I'm aware of 2 setups already
running in Marseille), because (at least this is what the people who did it
told me) it is quite easy to add to an existing TIRF setup (provided you
find software for the detection and localisation of individual fluorophores,
but there is now  an available ImageJ plugin for that).


--
Christophe Leterrier
Postdoc
INSERM UMR641 // Ionic channels Lab
IFR Jean Roche, Mediterranée University
Marseille, France
[hidden email]




On Sat, Jan 29, 2011 at 16:52, G. Esteban Fernandez <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> UCLA has a STED
>
> http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791
> <http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791>
> http://www.cnsi.ucla.edu/staticpages/core-facilities#alms
>
>
> <http://www.cnsi.ucla.edu/staticpages/core-facilities#alms>
>
> On Sat, Jan 29, 2011 at 7:32 AM, George McNamara
> <[hidden email]>wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Dear Confocal listserv,
> >
> > Who has purchased a fluorescence nanoscope? What can you tell me about
> your
> > experiences - either here or privately?
> >
> > I see 11 OMX labs listed at http://www.api.com/omx-labs.asp (may be out
> of
> > date or some customers may be shy).
> >
> > I am aware of STED systems in the USA at Yale Univ, NIH and I was told
> San
> > Diego (have not found where), and possibly UC Denver. Paul French
> apparently
> > did his own upgrade of a Leica SP2 (
> > http://www.focusonmicroscopy.org/2008/PDF/070_Auksorius.pdf and a Leica
> > Scientific Forum video ... see also related work at
> > ftp://ftp.espci.fr/incoming/Gilles/INSERM-STED-x6.pdf ).
> >
> > I am also aware of one 4pi microscope in the USA.
> >
> > So, who has N-SIM, N-STORM, SR-SIM, PALm, SR-200, and what can you tell
> me
> > about your experiences with your (purchased) nanoscope(s)?
> >
> > Sincerely,
> >
> > George
> > p.s. Please no need to clutter up the listserv with other people's
> > nanoscope references - I know how to use PubMed. For that matter, I've
> > replicated the results of the following two papers on my confocal's:
> >
> >   Subdiffraction fluorescence imaging of biomolecular structure and
> >   distributions with quantum dots. </pubmed/20600360>
> >
> >   Heidbreder M, Endesfelder U, van de Linde S, Hennig S, Widera D,
> >   Kaltschmidt B, Kaltschmidt C, Heilemann M.
> >
> >   Biochim Biophys Acta. 2010 Oct;1803(10):1224-9. Epub 2010 Jun
> >   23.PMID: 20600360
> >
> >
> >   Quantum dot triexciton imaging with three-dimensional subdiffraction
> >   resolution. </pubmed/19453186>
> >
> >   Hennig S, van de Linde S, Heilemann M, Sauer M.
> >
> >   Nano Lett. 2009 Jun;9(6):2466-70.PMID: 19453186
> >
> > I have used <1 Airy unit pinhole to improve triexciton resolution
> further.
> > I have not tried doing 3D deconvolution on the data.
> >
> >
> > --
> >
> >
> > George McNamara, PhD
> > Analytical Imaging Core Facility
> > University of Miami
> >
>

[Alberto Diaspro]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

At the Italian Institute of Technology, we got a Leica STED-CW and home built an FPALM on an inverted Nikon thanks to software sharing from Sam Hess Lab, University of Maine. We home built a WLL and a CW STED controlled by MPI Nanobiophotonics software. We are currently interested n the Nikon N-STORM.
All the best
Alby
Il giorno 29/gen/2011, alle ore 17.19, Christophe Leterrier ha scritto:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> The Bordeaux Imaging Center in Bordeaux, France has a STED and a TIRF/PALM
> http://www.bic.u-bordeaux2.fr/
>
> PALM/STORM setups are becoming more common (I'm aware of 2 setups already
> running in Marseille), because (at least this is what the people who did it
> told me) it is quite easy to add to an existing TIRF setup (provided you
> find software for the detection and localisation of individual fluorophores,
> but there is now  an available ImageJ plugin for that).
>
>
> --
> Christophe Leterrier
> Postdoc
> INSERM UMR641 // Ionic channels Lab
> IFR Jean Roche, Mediterranée University
> Marseille, France
> [hidden email]
>
>
>
>
> On Sat, Jan 29, 2011 at 16:52, G. Esteban Fernandez <
> [hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> UCLA has a STED
>>
>> http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791
>> <http://clms.cnsi.ucla.edu/cnsi/clms/equipment-list?search_lab=6791>
>> http://www.cnsi.ucla.edu/staticpages/core-facilities#alms
>>
>>
>> <http://www.cnsi.ucla.edu/staticpages/core-facilities#alms>
>>
>> On Sat, Jan 29, 2011 at 7:32 AM, George McNamara
>> <[hidden email]>wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> Dear Confocal listserv,
>>>
>>> Who has purchased a fluorescence nanoscope? What can you tell me about
>> your
>>> experiences - either here or privately?
>>>
>>> I see 11 OMX labs listed at http://www.api.com/omx-labs.asp (may be out
>> of
>>> date or some customers may be shy).
>>>
>>> I am aware of STED systems in the USA at Yale Univ, NIH and I was told
>> San
>>> Diego (have not found where), and possibly UC Denver. Paul French
>> apparently
>>> did his own upgrade of a Leica SP2 (
>>> http://www.focusonmicroscopy.org/2008/PDF/070_Auksorius.pdf and a Leica
>>> Scientific Forum video ... see also related work at
>>> ftp://ftp.espci.fr/incoming/Gilles/INSERM-STED-x6.pdf ).
>>>
>>> I am also aware of one 4pi microscope in the USA.
>>>
>>> So, who has N-SIM, N-STORM, SR-SIM, PALm, SR-200, and what can you tell
>> me
>>> about your experiences with your (purchased) nanoscope(s)?
>>>
>>> Sincerely,
>>>
>>> George
>>> p.s. Please no need to clutter up the listserv with other people's
>>> nanoscope references - I know how to use PubMed. For that matter, I've
>>> replicated the results of the following two papers on my confocal's:
>>>
>>> Subdiffraction fluorescence imaging of biomolecular structure and
>>> distributions with quantum dots. </pubmed/20600360>
>>>
>>> Heidbreder M, Endesfelder U, van de Linde S, Hennig S, Widera D,
>>> Kaltschmidt B, Kaltschmidt C, Heilemann M.
>>>
>>> Biochim Biophys Acta. 2010 Oct;1803(10):1224-9. Epub 2010 Jun
>>> 23.PMID: 20600360
>>>
>>>
>>> Quantum dot triexciton imaging with three-dimensional subdiffraction
>>> resolution. </pubmed/19453186>
>>>
>>> Hennig S, van de Linde S, Heilemann M, Sauer M.
>>>
>>> Nano Lett. 2009 Jun;9(6):2466-70.PMID: 19453186
>>>
>>> I have used <1 Airy unit pinhole to improve triexciton resolution
>> further.
>>> I have not tried doing 3D deconvolution on the data.
>>>
>>>
>>> --
>>>
>>>
>>> George McNamara, PhD
>>> Analytical Imaging Core Facility
>>> University of Miami
>>>
>>

ISTITUTO ITALIANO
DI TECNOLOGIA

Prof. Alberto Diaspro
Scientific Head
Nanophysics
Via Morego, 30 16163 Genova
Tel: [phone]
Fax [phone]
Mobile [phone]
www.iit.it
[hidden email]