Workshop Announcement; Advalytix

[Konz, Richard]

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Greetings everyone:

On Wednesday January 9^th the UMASS Medical School Flow Cytometry Core Facility will be hosting Advalytix, the global competence center for molecular analysis within Olympus.  Advalytix makes single cell molecular analysis solutions for research and clinical R&D and diagnostics and microfluidics solutions based on Advalytix’ patented surface acoustic wave technology.

The schedule will be as follows:

10:30-11:30 Flow Cytometry Core Facility [S5-322, Medical School Building, Worcester Campus]

Set-up PCR: hands-on pipetting micro liter GAPDH rtPCr assay onto slide with cells sorted by Ted Giehl on the BD FACSAria.

11:30-12:30 Seminar; Michelson conference room; first floor Lazare Research Building

Frank Feist, “Advalytix’ single cell molecular analysis platform. “  Please see abstract below.

2:30 -3:00 Flow Cytometry Core Facility 

Retrieve sample, run gel, interpret results, Q & A.

If you would like to attend the workshop, please RSVP me no later than Tuesday afternoon.  Please forward this email to a colleague who may benefit from this workshop.  Please feel free to just attend the seminar if your schedule does not permit the workshop.  Please contact me if you have any questions.

Best regards and Happy New Year,

-Rich

Richard F. Konz, Jr.
Assistant Professor
Department of Medicine
Graduate Faculty, Immunology and Virology Program
Director, Flow Cytometry Core Facility
UMass Medical School
55 Lake Avenue North
Room: S5-322
Worcester, MA 01655
Office:  508-856-1598
Lab:     508-856-3276
Fax:   [phone]
Email:  [hidden email]
Lab Web:      http://www.umassmed.edu/facslab
Faculty Web: http://www.umassmed.edu/ivp/faculty/konz.cfm

Talk abstract:

We will present Advalytix’ single cell molecular analysis platform: the AmplGrid slide and AmpliSpeed slide cycler.  The platform enables highly sensitive amplification of DNA, mRNA, or mtDNA from single cells in a simple workflow.  Cells are deposited onto the AmpliGrid slide via FACS, laser capture microscopy, or micromanipulator.  After adding a 1 micro liter assay – either manually with a pipette or with an available automated liquid handler solution – AmpliGrid slides are thermo cycled on the world’s smallest thermocycler – the AmpliSpeed.  Post PCR, amplicons are retrieved from the slide and analyzed via gel, capillary electrophoresis or sequencing.  We will present case studies from immunology, oncology, stem cells, and metabolic disease that highlight the advantage of AmpliGrid single cell analysis vs. conventional pooled-sample approaches.