*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopyPost images on
http://www.imgur.com and include the link in your posting.
*****
Sounds like a technical issue. 470nm should work well for YFP. It's either
a filter problem or the fluorophore was a dud.
Craig
On Wed, Jan 14, 2015 at 10:50 AM, Aryeh Weiss <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> A colleague of mine told me that he is unable to see YFP/Venus with his
> LED source (Lumencore, 470nm LED). This surprises me, even if 470nm is not
> at the excitation peak of YFP.
> Are there list-members who use LED excitation sources with YFP? If so, I
> would appreciate if they would share their experience with me.
>
> Thanks in advance.
> --aryeh
>
Locked
