Yokogawa CSU-W1

[Vitaly Boyko-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

 
Dear All,
We are looking for a feedback on the latest spinning disk confocal unit by Yokogawa. Any feedback on its performance, setup, stability, frequency of maintenance, quality of service will be appreciated.
Many thanks in advance.
Vitaly
MSKCCT: 212-639-6667   
 

[Montero Llopis, Paula De La Milagrosa]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Vitaly,

We have a Yokowaga CSU-W1 on a Nikon Ti with an Andor Zyla 4.2 plus and are very happy with it. We have a Toptica 4 laser line laser launch that is easy to use and align. Pinhole crosstalk with the plan apo 100x/1.4 objective is negligible (at least with the bead samples we used to test it) and it does a great job even in lower NA lenses, at least compared to the CSU-X1. Setting it up was pretty straightforward.

Dust is a bit of an issue, we have to wipe the dichroic mirrors once every couple of months. Haven't noticed as big issue with dust in the pinholes, but I think other people have reported this.

Rolloff is not awful, but we also have a macro in place to crop the ROI, so that users don't have to think about it.

We got our system through MVI/Nikon and they do a great job supporting it.

I hope this helps!

Best,

Paula

Paula Montero Llopis, PhD
MicRoN Core Director
micron.hms.harvard.edu

> On Feb 15, 2019, at 08:13, Vitaly Boyko <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> Dear All,
> We are looking for a feedback on the latest spinning disk confocal unit by Yokogawa. Any feedback on its performance, setup, stability, frequency of maintenance, quality of service will be appreciated.
> Many thanks in advance.
> Vitaly
> MSKCCT: 212-639-6667    
>

[Sylvie Le Guyader]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Paula

I am interested in the dust problem you mention. My understanding is that this problem was fixed by Yokogawa and that they even apply their fix to older W1 for free if one complains.
How old is your W1 spinning disk? Did you have to go through the fix or have you never had the problem?

Another question regarding the pinhole cross talk: do you know how deep in your bead sample you were where you had negligible cross talk?

thanks

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7C,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website
Follow our microscopy blog!



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Montero Llopis, Paula De La Milagrosa
Sent: den 19 februari 2019 05:50
To: [hidden email]
Subject: Re: Yokogawa CSU-W1

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Vitaly,

We have a Yokowaga CSU-W1 on a Nikon Ti with an Andor Zyla 4.2 plus and are very happy with it. We have a Toptica 4 laser line laser launch that is easy to use and align. Pinhole crosstalk with the plan apo 100x/1.4 objective is negligible (at least with the bead samples we used to test it) and it does a great job even in lower NA lenses, at least compared to the CSU-X1. Setting it up was pretty straightforward.

Dust is a bit of an issue, we have to wipe the dichroic mirrors once every couple of months. Haven't noticed as big issue with dust in the pinholes, but I think other people have reported this.

Rolloff is not awful, but we also have a macro in place to crop the ROI, so that users don't have to think about it.

We got our system through MVI/Nikon and they do a great job supporting it.

I hope this helps!

Best,

Paula

Paula Montero Llopis, PhD
MicRoN Core Director
micron.hms.harvard.edu

> On Feb 15, 2019, at 08:13, Vitaly Boyko <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> Dear All,
> We are looking for a feedback on the latest spinning disk confocal unit by Yokogawa. Any feedback on its performance, setup, stability, frequency of maintenance, quality of service will be appreciated.
> Many thanks in advance.
> Vitaly
> MSKCCT: 212-639-6667
>

När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>.


Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>.

[Ivo Telley]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Sylvie

I wish to comment on this matter as we were one of the first purchasers of the W1 system. The dust problem has not been fixed to a satisfactory level for the W1 system. It is true that Yokogawa has “designed” and provided a fix, which essentially consists of a bunch of metal shields to try and compartmentalise better the interior of the scanner (i.e. electronics, ventilators, optics). However, in our experience the problem remains and after an intense cleaning by a technician the disk collects dust particles after 1-2 weeks, and we sometimes see bright auto-fluorescent particles in individual frames. The bright-spot problem gets worse within months and makes image processing unnecessary complicated.
I must stress here that this dust problem is caused by a suboptimal design of the entire optical compartment and its air sealing. This problem cannot be fixed easily.

Regards,
Ivo A. Telley, PhD
Instituto Gulbenkian de Ciência (IGC)
Rua da Quinta Grande, 6
2780-156 Oeiras, Portugal
Phone: [phone]
http://www.igc.gulbenkian.pt/itelley

> On 1 Mar 2019, at 11:22, Sylvie Le Guyader <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Paula
>
> I am interested in the dust problem you mention. My understanding is that this problem was fixed by Yokogawa and that they even apply their fix to older W1 for free if one complains.
> How old is your W1 spinning disk? Did you have to go through the fix or have you never had the problem?
>
> Another question regarding the pinhole cross talk: do you know how deep in your bead sample you were where you had negligible cross talk?
>
> thanks
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Hälsovägen 7C,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI website
> Follow our microscopy blog!
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On Behalf Of Montero Llopis, Paula De La Milagrosa
> Sent: den 19 februari 2019 05:50
> To: [hidden email]
> Subject: Re: Yokogawa CSU-W1
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Vitaly,
>
> We have a Yokowaga CSU-W1 on a Nikon Ti with an Andor Zyla 4.2 plus and are very happy with it. We have a Toptica 4 laser line laser launch that is easy to use and align. Pinhole crosstalk with the plan apo 100x/1.4 objective is negligible (at least with the bead samples we used to test it) and it does a great job even in lower NA lenses, at least compared to the CSU-X1. Setting it up was pretty straightforward.
>
> Dust is a bit of an issue, we have to wipe the dichroic mirrors once every couple of months. Haven't noticed as big issue with dust in the pinholes, but I think other people have reported this.
>
> Rolloff is not awful, but we also have a macro in place to crop the ROI, so that users don't have to think about it.
>
> We got our system through MVI/Nikon and they do a great job supporting it.
>
> I hope this helps!
>
> Best,
>
> Paula
>
> Paula Montero Llopis, PhD
> MicRoN Core Director
> micron.hms.harvard.edu
>
>> On Feb 15, 2019, at 08:13, Vitaly Boyko <[hidden email]> wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>>
>> Dear All,
>> We are looking for a feedback on the latest spinning disk confocal unit by Yokogawa. Any feedback on its performance, setup, stability, frequency of maintenance, quality of service will be appreciated.
>> Many thanks in advance.
>> Vitaly
>> MSKCCT: 212-639-6667
>>
>
>
> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>.
>
>
> Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>.

[Montero Llopis, Paula De La Milagrosa]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Sylvie,

We haven't had issues with dust on te pinholes, but we do notice dust on the dichroic mirrors. Those we clean with a swab wrapped in lens paper.

With the 100x lens and bead sample I didn't see pinhole crosstalk in at least a range of 10-15 um (I will have to check the stacks). And in other magnifications, in which you can detect pinhole crosstalk (very bright 15 um  fluorescence spheres) you still get pretty good images without a really bad degradation of the image. I will find and send you the images I took with the 20x/075 objective of Drosophila embryos (90 um thick). At the end of the stack you do see spherical aberration and probably some loss in contrast due to pinhole crosstalk, but I was very satisfied with its performance. I love this system.

Hope this helps

Best,

Paula

> On Mar 1, 2019, at 06:23, Sylvie Le Guyader <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Paula
>
> I am interested in the dust problem you mention. My understanding is that this problem was fixed by Yokogawa and that they even apply their fix to older W1 for free if one complains.
> How old is your W1 spinning disk? Did you have to go through the fix or have you never had the problem?
>
> Another question regarding the pinhole cross talk: do you know how deep in your bead sample you were where you had negligible cross talk?
>
> thanks
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Hälsovägen 7C,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI website
> Follow our microscopy blog!
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On Behalf Of Montero Llopis, Paula De La Milagrosa
> Sent: den 19 februari 2019 05:50
> To: [hidden email]
> Subject: Re: Yokogawa CSU-W1
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Vitaly,
>
> We have a Yokowaga CSU-W1 on a Nikon Ti with an Andor Zyla 4.2 plus and are very happy with it. We have a Toptica 4 laser line laser launch that is easy to use and align. Pinhole crosstalk with the plan apo 100x/1.4 objective is negligible (at least with the bead samples we used to test it) and it does a great job even in lower NA lenses, at least compared to the CSU-X1. Setting it up was pretty straightforward.
>
> Dust is a bit of an issue, we have to wipe the dichroic mirrors once every couple of months. Haven't noticed as big issue with dust in the pinholes, but I think other people have reported this.
>
> Rolloff is not awful, but we also have a macro in place to crop the ROI, so that users don't have to think about it.
>
> We got our system through MVI/Nikon and they do a great job supporting it.
>
> I hope this helps!
>
> Best,
>
> Paula
>
> Paula Montero Llopis, PhD
> MicRoN Core Director
> micron.hms.harvard.edu
>
>> On Feb 15, 2019, at 08:13, Vitaly Boyko <[hidden email]> wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>>
>> Dear All,
>> We are looking for a feedback on the latest spinning disk confocal unit by Yokogawa. Any feedback on its performance, setup, stability, frequency of maintenance, quality of service will be appreciated.
>> Many thanks in advance.
>> Vitaly
>> MSKCCT: 212-639-6667
>>
>
>
> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>.
>
>
> Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>.

[Manabu Fueki]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Ivo,



Thank you very much for your patronage of our products.

I’m the QA manager of CSU series.

Would you please let me know the Serial No. of the CSU-W1?

And who is taking care of the dust problem?

I think we’ve deployed our latest countermeasure for the problem through our business partners.

If you have any problem, we will have to investigate the cause.



Best regards,

Manabu



********************************************************************

Manabu Fueki

Yokogawa Electric Corporation

Life Innovation Business HQ

QA & Customer Support Center

[hidden email]

2-3 Hokuyodai, Kanazawa-shi, Ishikawa, 920-0177 Japan

Tel:[phone]  Fax:[phone]
Follow Us : Twitter<https://twitter.com/Yokogawa_LS> | Facebook<https://www.facebook.com/yokogawa.life.science/> | Linkedin<https://www.linkedin.com/company/yokogawa-life-science/>

https://www.yokogawa.com/solutions/products-platforms/life-science/

********************************************************************



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ivo Telley
Sent: Friday, March 1, 2019 8:35 PM
To: [hidden email]
Subject: Re: Yokogawa CSU-W1



*****

To join, leave or search the confocal microscopy listserv, go to:

http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

Post images on http://www.imgur.com and include the link in your posting.

*****



Dear Sylvie



I wish to comment on this matter as we were one of the first purchasers of the W1 system. The dust problem has not been fixed to a satisfactory level for the W1 system. It is true that Yokogawa has “designed” and provided a fix, which essentially consists of a bunch of metal shields to try and compartmentalise better the interior of the scanner (i.e. electronics, ventilators, optics). However, in our experience the problem remains and after an intense cleaning by a technician the disk collects dust particles after 1-2 weeks, and we sometimes see bright auto-fluorescent particles in individual frames. The bright-spot problem gets worse within months and makes image processing unnecessary complicated.

I must stress here that this dust problem is caused by a suboptimal design of the entire optical compartment and its air sealing. This problem cannot be fixed easily.



Regards,

Ivo A. Telley, PhD

Instituto Gulbenkian de Ciência (IGC)

Rua da Quinta Grande, 6

2780-156 Oeiras, Portugal

Phone: [phone]

http://www.igc.gulbenkian.pt/itelley



> On 1 Mar 2019, at 11:22, Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> wrote:

>

> *****

> To join, leave or search the confocal microscopy listserv, go to:

> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

> Post images on http://www.imgur.com and include the link in your posting.

> *****

>

> Hi Paula

>

> I am interested in the dust problem you mention. My understanding is that this problem was fixed by Yokogawa and that they even apply their fix to older W1 for free if one complains.

> How old is your W1 spinning disk? Did you have to go through the fix or have you never had the problem?

>

> Another question regarding the pinhole cross talk: do you know how deep in your bead sample you were where you had negligible cross talk?

>

> thanks

>

> Med vänlig hälsning / Best regards

>

> Sylvie

>

> @@@@@@@@@@@@@@@@@@@@@@@@

> Sylvie Le Guyader, PhD

> Live Cell Imaging Facility Manager

> Karolinska Institutet- Bionut Dpt

> Hälsovägen 7C,

> Room 7362 (lab)/7840 (office)

> 14157 Huddinge, Sweden

> mobile: [phone]

> LCI website

> Follow our microscopy blog!

>

>

>

> -----Original Message-----

> From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> On Behalf Of Montero Llopis, Paula De La Milagrosa

> Sent: den 19 februari 2019 05:50

> To: [hidden email]<mailto:[hidden email]>

> Subject: Re: Yokogawa CSU-W1

>

> *****

> To join, leave or search the confocal microscopy listserv, go to:

> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

> Post images on http://www.imgur.com and include the link in your posting.

> *****

>

> Dear Vitaly,

>

> We have a Yokowaga CSU-W1 on a Nikon Ti with an Andor Zyla 4.2 plus and are very happy with it. We have a Toptica 4 laser line laser launch that is easy to use and align. Pinhole crosstalk with the plan apo 100x/1.4 objective is negligible (at least with the bead samples we used to test it) and it does a great job even in lower NA lenses, at least compared to the CSU-X1. Setting it up was pretty straightforward.

>

> Dust is a bit of an issue, we have to wipe the dichroic mirrors once every couple of months. Haven't noticed as big issue with dust in the pinholes, but I think other people have reported this.

>

> Rolloff is not awful, but we also have a macro in place to crop the ROI, so that users don't have to think about it.

>

> We got our system through MVI/Nikon and they do a great job supporting it.

>

> I hope this helps!

>

> Best,

>

> Paula

>

> Paula Montero Llopis, PhD

> MicRoN Core Director

> micron.hms.harvard.edu

>

>> On Feb 15, 2019, at 08:13, Vitaly Boyko <[hidden email]<mailto:[hidden email]>> wrote:

>>

>> *****

>> To join, leave or search the confocal microscopy listserv, go to:

>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

>> Post images on http://www.imgur.com and include the link in your posting.

>> *****

>>

>>

>> Dear All,

>> We are looking for a feedback on the latest spinning disk confocal unit by Yokogawa. Any feedback on its performance, setup, stability, frequency of maintenance, quality of service will be appreciated.

>> Many thanks in advance.

>> Vitaly

>> MSKCCT: 212-639-6667

>>

>

>

> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>.

>

>

> Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>.

-----
CONFIDENTIAL: This e-mail may contain information that is confidential or otherwise protected from disclosure and intended only for the party to whom it is addressed. If you are not the intended recipient, please notify the sender by return and delete this e-mail. You are hereby formally advised that any unauthorized use, disclosure or copying of this email is strictly prohibited and may be unlawful.

[lgelman]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Sylvie, Hi Vitaly, Hi Paula, all,

I support what Ivo and Paula said: we had 3 CSU-W1 on the facility (plus a CV7000), and despite the installation of the dust-protection system (whatever that is) by Yokogawa a few months ago, we still get dust on the disks (maybe less often though), or on the dichroics. It is very costly to have someone coming and cleaning them.

Except that, we are extremely happy with the CSU-W1s. These have become the working horses for confocal imaging on the facility. People have abandoned the LSMs, except for some specific applications (e.g. FCS, acceptor photobleaching, SHG). Even in thick samples (i.e. >100uM brain slices) we get very nice confocality and resolution. For our applications and samples, image quality is as good or better as before (with LSMs), but we are 20x times faster, which is a big advantage in general but in particular for people doing connectomics and tiling over very large regions. The large field of view, the bigger distance between pinholes and the two motorized disks have bene game changers for the CSU-scan heads. We have also two CSU-X1 but these are really limited to their application "niche", i.e. live cell imaging with a 100x/1.4.
At the beginning, I thought the X1s would still be better than the W1 for sensitivity and speed, but this is not what I observed. Maybe it depends there of the kind of sample. Our CSU-W1 units have either two PCO Edge, two Ultra 88 or two Prime95B mounted and this makes a big difference, depending if you seek speed or sensitivity.

Service, stability, etc. depends on by whom you'll purchase your systems I guess. I got mine at Visitron.

Best regards,

Laurent.


Laurent Gelman
Facility for Advanced Imaging and Microscopy
Head Light Microscopy
Friedrich Miescher Institute for Biomedical Research
Maulbeerstrasse 66
4058 basel
Switzerland
[phone]

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Ivo Telley
Sent: Friday, March 1, 2019 12:35
To: [hidden email]
Subject: Re: Yokogawa CSU-W1

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Sylvie

I wish to comment on this matter as we were one of the first purchasers of the W1 system. The dust problem has not been fixed to a satisfactory level for the W1 system. It is true that Yokogawa has “designed” and provided a fix, which essentially consists of a bunch of metal shields to try and compartmentalise better the interior of the scanner (i.e. electronics, ventilators, optics). However, in our experience the problem remains and after an intense cleaning by a technician the disk collects dust particles after 1-2 weeks, and we sometimes see bright auto-fluorescent particles in individual frames. The bright-spot problem gets worse within months and makes image processing unnecessary complicated.
I must stress here that this dust problem is caused by a suboptimal design of the entire optical compartment and its air sealing. This problem cannot be fixed easily.

Regards,
Ivo A. Telley, PhD
Instituto Gulbenkian de Ciência (IGC)
Rua da Quinta Grande, 6
2780-156 Oeiras, Portugal
Phone: [phone]
http://www.igc.gulbenkian.pt/itelley

> On 1 Mar 2019, at 11:22, Sylvie Le Guyader <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Paula
>
> I am interested in the dust problem you mention. My understanding is that this problem was fixed by Yokogawa and that they even apply their fix to older W1 for free if one complains.
> How old is your W1 spinning disk? Did you have to go through the fix or have you never had the problem?
>
> Another question regarding the pinhole cross talk: do you know how deep in your bead sample you were where you had negligible cross talk?
>
> thanks
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Hälsovägen 7C,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI website
> Follow our microscopy blog!
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List <[hidden email]> On Behalf Of Montero Llopis, Paula De La Milagrosa
> Sent: den 19 februari 2019 05:50
> To: [hidden email]
> Subject: Re: Yokogawa CSU-W1
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Vitaly,
>
> We have a Yokowaga CSU-W1 on a Nikon Ti with an Andor Zyla 4.2 plus and are very happy with it. We have a Toptica 4 laser line laser launch that is easy to use and align. Pinhole crosstalk with the plan apo 100x/1.4 objective is negligible (at least with the bead samples we used to test it) and it does a great job even in lower NA lenses, at least compared to the CSU-X1. Setting it up was pretty straightforward.
>
> Dust is a bit of an issue, we have to wipe the dichroic mirrors once every couple of months. Haven't noticed as big issue with dust in the pinholes, but I think other people have reported this.
>
> Rolloff is not awful, but we also have a macro in place to crop the ROI, so that users don't have to think about it.
>
> We got our system through MVI/Nikon and they do a great job supporting it.
>
> I hope this helps!
>
> Best,
>
> Paula
>
> Paula Montero Llopis, PhD
> MicRoN Core Director
> micron.hms.harvard.edu
>
>> On Feb 15, 2019, at 08:13, Vitaly Boyko <[hidden email]> wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>>
>> Dear All,
>> We are looking for a feedback on the latest spinning disk confocal unit by Yokogawa. Any feedback on its performance, setup, stability, frequency of maintenance, quality of service will be appreciated.
>> Many thanks in advance.
>> Vitaly
>> MSKCCT: 212-639-6667
>>
>
>
> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://ki.se/medarbetare/integritetsskyddspolicy>.
>
>
> Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI’s processing of personal data here<https://ki.se/en/staff/data-protection-policy>.