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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear all, We have a good old Yokogawa QLC100 Spinning Disk unit that we wish to connect to a custom setup, but it is impossible to find documentation on the unit other than brochures and summary specs. More importantly I would like to know the kind of input/output the SYNC BNC connector is capable of providing. If anyone has some documentation on this machine, it would be highly appreaciated All the best Olivier Burri |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** *** VENDOR RESPONSE *** Hi Olivier, My understanding from Yokogawa on this (BNC sync) issue is the following: It's an input that synchronises the disk speed across a limited range (input in the PAL/NTSC frequency range of ~25 - 83 Hz - yes, I realise the disk speed is approx. 30Hz). It can only be connected to a video camera and/or monitor using a BNC T-piece and should be terminated with a 75 Ohm connector if necessary (if the CSU is the last in the chain). Given that video (composite / BNC) cameras are somewhat before my time (I have not used them with CSUs, but have used them with some older / budget widefield systems), I cannot comment on whether or not this connector is of any use. The CSU-10/22 manual has this to say on the matter: " If you want to synchronize the scanning speed of the scanner unit to the video rate of a CCD camera, etc., establish connections as shown in Figure 3-3 using the BNC-T connector provided. For the video signal connections, only one terminator will be required. The CSU22 is not designed to serve as a video signal terminator, so if you want to use it for termination, use the 75Ω connector provided." "Video sync signal - NTSC or PAL composite signal - Input - 1Vpp Input impedance: 10 kΩ or more" In my opinion, if you plan to use the CSU with a modern EMCCD or sCMOS, your best bet is to do the following: 1) Synchronise the camera to the light source - e.g. ensure that excitation laser is only enabled during exposure. This is easy with an AOTF or suitably fast laser. 2) Try different physical rotations of the camera on the CSU to ensure the readout direction and spinning disk directions result in the least amount of streaking. 3) Try different exposure times (both whole and fractions of a ms) to ensure optimal (i.e. minimal streaking) image quality. In theory, the CSU-10 produces a complete image every 2.8 ms. Feel free to contact me on or off-list for further information. Best regards, James James Wainwright Product Support Engineer (Microscopy Systems) Europe, Middle East & Africa Tel: +44 (0) 2890 270 873 Mob: +44 (0) 7834 710 834 Fax: +44 (0) 2890 310 792 Web: www.andor.com/ContactSupport.aspx?type=s -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Burri Olivier Sent: 01 September 2015 12:59 To: [hidden email] Subject: Yokogawa QLC100 specs, ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear all, We have a good old Yokogawa QLC100 Spinning Disk unit that we wish to connect to a custom setup, but it is impossible to find documentation on the unit other than brochures and summary specs. More importantly I would like to know the kind of input/output the SYNC BNC connector is capable of providing. If anyone has some documentation on this machine, it would be highly appreaciated All the best Olivier Burri This message has been scanned for malware by SurfControl plc. www.surfcontrol.com |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi James, Thanks for the info and all the advice! The descriptions you provide are indeed what we have currently setup for the system (sync acquisition to excitation light) and make sure that the camera is exposed for multiples of the disk rotation. I haven't tried the part where you try different rotations of the camera, but I will definitely give that a shot if it can help reduce streaking as well. You mention that you have a manual for a CSU22, which transitions perfectly to my next question. I would be interested in knowing the pinout for a CSU21-22. Would it be possible for you to send me this manual? All the best and thanks again! Oli > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of > WAINWRIGHT James > Sent: Tuesday, September 1, 2015 14:38 > To: [hidden email] > Subject: Re: Yokogawa QLC100 specs, > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > *** VENDOR RESPONSE *** > > Hi Olivier, > > My understanding from Yokogawa on this (BNC sync) issue is the following: > > It's an input that synchronises the disk speed across a limited range (input in > the PAL/NTSC frequency range of ~25 - 83 Hz - yes, I realise the disk speed is > approx. 30Hz). > It can only be connected to a video camera and/or monitor using a BNC T- > piece and should be terminated with a 75 Ohm connector if necessary (if the > CSU is the last in the chain). > > Given that video (composite / BNC) cameras are somewhat before my time > (I have not used them with CSUs, but have used them with some older / > budget widefield systems), I cannot comment on whether or not this > connector is of any use. > > The CSU-10/22 manual has this to say on the matter: > > " If you want to synchronize the scanning speed of the scanner unit to the > video rate of a CCD camera, etc., establish connections as shown in Figure 3-3 > using the BNC-T connector provided. For the video signal connections, only > one terminator will be required. The CSU22 is not designed to serve as a > video signal terminator, so if you want to use it for termination, use the 75Ω > connector provided." > > "Video sync signal - NTSC or PAL composite signal - Input - 1Vpp Input > impedance: 10 kΩ or more" > > In my opinion, if you plan to use the CSU with a modern EMCCD or sCMOS, > your best bet is to do the following: > > 1) Synchronise the camera to the light source - e.g. ensure that excitation > laser is only enabled during exposure. This is easy with an AOTF or suitably > fast laser. > > 2) Try different physical rotations of the camera on the CSU to ensure the > readout direction and spinning disk directions result in the least amount of > streaking. > > 3) Try different exposure times (both whole and fractions of a ms) to ensure > optimal (i.e. minimal streaking) image quality. In theory, the CSU-10 produces > a complete image every 2.8 ms. > > Feel free to contact me on or off-list for further information. > > Best regards, > > James > > James Wainwright > Product Support Engineer (Microscopy Systems) Europe, Middle East & Africa > > Tel: +44 (0) 2890 270 873 > Mob: +44 (0) 7834 710 834 > Fax: +44 (0) 2890 310 792 > Web: www.andor.com/ContactSupport.aspx?type=s > > > > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of Burri Olivier > Sent: 01 September 2015 12:59 > To: [hidden email] > Subject: Yokogawa QLC100 specs, > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear all, > > We have a good old Yokogawa QLC100 Spinning Disk unit that we wish to > connect to a custom setup, but it is impossible to find documentation on the > unit other than brochures and summary specs. > > More importantly I would like to know the kind of input/output the SYNC > BNC connector is capable of providing. > > If anyone has some documentation on this machine, it would be highly > appreaciated > > All the best > > Olivier Burri > > > This message has been scanned for malware by SurfControl plc. > www.surfcontrol.com |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** *** VENDOR RESPONSE *** Hi Oli, Sure thing, here is what I have (it may be useful for others too, although I should add the disclaimer that I cannot guarantee the accuracy of the contents and that the CSU10/21/22 are no longer supported by Yokogawa): Pinout: https://www.dropbox.com/s/ewq84c5z1bkicbe/CSU_22_Manual_25_pin_IO.pdf?dl=0 Sync BNC: https://www.dropbox.com/s/k3y6w3gbg25783u/CSU_22_video_BNC.pdf?dl=0 Full manual: https://www.dropbox.com/s/yxihb7j2on3pdx1/CSU_22_Manual_IM87A7C22-01E.pdf?dl=0 James James Wainwright Product Support Engineer (Microscopy Systems) Europe, Middle East & Africa Tel: +44 (0) 2890 270 873 Mob: +44 (0) 7834 710 834 Fax: +44 (0) 2890 310 792 Web: www.andor.com/ContactSupport.aspx?type=s -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Burri Olivier Sent: 02 September 2015 08:01 To: [hidden email] Subject: Re: Yokogawa QLC100 specs, ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi James, Thanks for the info and all the advice! The descriptions you provide are indeed what we have currently setup for the system (sync acquisition to excitation light) and make sure that the camera is exposed for multiples of the disk rotation. I haven't tried the part where you try different rotations of the camera, but I will definitely give that a shot if it can help reduce streaking as well. You mention that you have a manual for a CSU22, which transitions perfectly to my next question. I would be interested in knowing the pinout for a CSU21-22. Would it be possible for you to send me this manual? All the best and thanks again! Oli > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of WAINWRIGHT > James > Sent: Tuesday, September 1, 2015 14:38 > To: [hidden email] > Subject: Re: Yokogawa QLC100 specs, > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > *** VENDOR RESPONSE *** > > Hi Olivier, > > My understanding from Yokogawa on this (BNC sync) issue is the following: > > It's an input that synchronises the disk speed across a limited range > (input in the PAL/NTSC frequency range of ~25 - 83 Hz - yes, I realise > the disk speed is approx. 30Hz). > It can only be connected to a video camera and/or monitor using a BNC > T- piece and should be terminated with a 75 Ohm connector if necessary > (if the CSU is the last in the chain). > > Given that video (composite / BNC) cameras are somewhat before my time > (I have not used them with CSUs, but have used them with some older / > budget widefield systems), I cannot comment on whether or not this > connector is of any use. > > The CSU-10/22 manual has this to say on the matter: > > " If you want to synchronize the scanning speed of the scanner unit to > the video rate of a CCD camera, etc., establish connections as shown > in Figure 3-3 using the BNC-T connector provided. For the video signal > connections, only one terminator will be required. The CSU22 is not > designed to serve as a video signal terminator, so if you want to use > it for termination, use the 75Ω connector provided." > > "Video sync signal - NTSC or PAL composite signal - Input - 1Vpp > Input > impedance: 10 kΩ or more" > > In my opinion, if you plan to use the CSU with a modern EMCCD or > sCMOS, your best bet is to do the following: > > 1) Synchronise the camera to the light source - e.g. ensure that > excitation laser is only enabled during exposure. This is easy with an > AOTF or suitably fast laser. > > 2) Try different physical rotations of the camera on the CSU to ensure > the readout direction and spinning disk directions result in the least > amount of streaking. > > 3) Try different exposure times (both whole and fractions of a ms) to > ensure optimal (i.e. minimal streaking) image quality. In theory, the > CSU-10 produces a complete image every 2.8 ms. > > Feel free to contact me on or off-list for further information. > > Best regards, > > James > > James Wainwright > Product Support Engineer (Microscopy Systems) Europe, Middle East & > Africa > > Tel: +44 (0) 2890 270 873 > Mob: +44 (0) 7834 710 834 > Fax: +44 (0) 2890 310 792 > Web: www.andor.com/ContactSupport.aspx?type=s > > > > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of Burri Olivier > Sent: 01 September 2015 12:59 > To: [hidden email] > Subject: Yokogawa QLC100 specs, > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear all, > > We have a good old Yokogawa QLC100 Spinning Disk unit that we wish to > connect to a custom setup, but it is impossible to find documentation > on the unit other than brochures and summary specs. > > More importantly I would like to know the kind of input/output the > SYNC BNC connector is capable of providing. > > If anyone has some documentation on this machine, it would be highly > appreaciated > > All the best > > Olivier Burri > > > This message has been scanned for malware by SurfControl plc. > www.surfcontrol.com |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear James Thank you very much for the information! The RS232 commands work flawlessly and I got it to run under LabView in no time. Thanks again! Oli > Hi Oli, > > Sure thing, here is what I have (it may be useful for others too, although I > should add the disclaimer that I cannot guarantee the accuracy of the > contents and that the CSU10/21/22 are no longer supported by Yokogawa): > > Pinout: > > https://www.dropbox.com/s/ewq84c5z1bkicbe/CSU_22_Manual_25_pin_IO > .pdf?dl=0 > > Sync BNC: > > https://www.dropbox.com/s/k3y6w3gbg25783u/CSU_22_video_BNC.pdf?dl > =0 > > Full manual: > > https://www.dropbox.com/s/yxihb7j2on3pdx1/CSU_22_Manual_IM87A7C2 > 2-01E.pdf?dl=0 > > James > > > James Wainwright > Product Support Engineer (Microscopy Systems) Europe, Middle East & Africa > > Tel: +44 (0) 2890 270 873 > Mob: +44 (0) 7834 710 834 > Fax: +44 (0) 2890 310 792 > Web: www.andor.com/ContactSupport.aspx?type=s |
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