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First, have the manufacturers park each system in your lab for two weeks.
We have just finished testing the Zeiss 780 and the Leica SP5II with
HyD. Both the GASP and the HyD are far more sensitive and less noisy
than the conventional PMT's; for weak signals or fast-scan/low-dose/live
cell, they are quite an improvement. If you'd like more details,
contact me off-line.
Julian
On 2/17/11 8:09 AM, allison van-de-meene (RRes-Roth) wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> *****
>
> Hello,
> We are in the process of reviewing the current confocal microscopes on the market for purchase this year. We are after a system that has good capabilities in separating and characterizing autofluorescence from the fluorescent signals of interest (preferably whilst scanning in live mode) as well as having high sensitivity to detect levels of expression that change over time.
> The options are the Zeiss 780, Leica SP5 II with the hybrid detectors or the Nikon A1R. I would be interested in hearing peoples ideas etc about these systems.
> Thanks for your help.
> Allison
--
Julian P.S. Smith III
Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC 29733
803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)
Research Website www.birdnest.org/smithj
Personal Website www.rociada-east.net
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