Zeiss Cell Observer 7

[SUBSCRIBE CONFOCALMICROSCOPY Anonymous-3]

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Dear All,

Zeiss offers Definite Focus.2 in their live cell imaging system Cell Observer 7. How does it compare with the hardware auto-focus from other vendors? I have used PFS from Nikon and believe that PFS is the gold standard in hardware auto-focus.The local vendors says "Definite Focus.2 is an option. The system works fine even without it - software auto focus is enough". Is it true? Given the fact that one usually images overnight with live cell microscope, "Cell Observer 7 is stable and robust without hardware auto-focus" is unbelievable! Please clarify.

Best regards,
Core Imaging Facility Staff
NTU

[Sylvie Le Guyader]

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Hardware autofocus is not only use for life Cell Imaging. We use it routinely to image fixed samples. If the sample is over 3 fields of view, it is likely that some refocussing will be needed. This is especially true for multiwell plates and 35 mm dishes which unfortunately are never flat. Software autofocus bleaches your sample. It is useful in situations where HW autofocus cannot be used.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@

Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Hälsovägen 7,
Novum, G lift, floor 6
14157<tel:14157> Huddinge
Sweden
mobile: [phone]<tel:+46%20(0)%2073%20733%205008>
office: [phone]<tel:+46%20(0)%2008-524%20811%2072>
LCI website

---- SUBSCRIBE CONFOCALMICROSCOPY Anonymous wrote ----

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Dear All,

Zeiss offers Definite Focus.2 in their live cell imaging system Cell Observer 7. How does it compare with the hardware auto-focus from other vendors? I have used PFS from Nikon and believe that PFS is the gold standard in hardware auto-focus.The local vendors says "Definite Focus.2 is an option. The system works fine even without it - software auto focus is enough". Is it true? Given the fact that one usually images overnight with live cell microscope, "Cell Observer 7 is stable and robust without hardware auto-focus" is unbelievable! Please clarify.

Best regards,
Core Imaging Facility Staff
NTU

[Dr. K N Ganesh]

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In my opinion software autofocus is not fast whereas the hardware works based on the Far Red low power iR led or laser which focused onto the coverslip not on the sample.hence the low energy carrying IR photons will have minimal damage on the sample which has absorption max at visible range. The advantage is instantaneous autofocus for live specimens.  Some companies also offer continuous autofocus..
It's worth testing live specimen without any interval in both systems and decide.
Reg
Ganesh

Sent from my iPhone

> On 24-Jul-2017, at 12:42 PM, Sylvie Le Guyader <[hidden email]> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hardware autofocus is not only use for life Cell Imaging. We use it routinely to image fixed samples. If the sample is over 3 fields of view, it is likely that some refocussing will be needed. This is especially true for multiwell plates and 35 mm dishes which unfortunately are never flat. Software autofocus bleaches your sample. It is useful in situations where HW autofocus cannot be used.
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
>
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Hälsovägen 7,
> Novum, G lift, floor 6
> 14157<tel:14157> Huddinge
> Sweden
> mobile: [phone]<tel:+46%20(0)%2073%20733%205008>
> office: [phone]<tel:+46%20(0)%2008-524%20811%2072>
> LCI website
>
> ---- SUBSCRIBE CONFOCALMICROSCOPY Anonymous wrote ----
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
> Zeiss offers Definite Focus.2 in their live cell imaging system Cell Observer 7. How does it compare with the hardware auto-focus from other vendors? I have used PFS from Nikon and believe that PFS is the gold standard in hardware auto-focus.The local vendors says "Definite Focus.2 is an option. The system works fine even without it - software auto focus is enough". Is it true? Given the fact that one usually images overnight with live cell microscope, "Cell Observer 7 is stable and robust without hardware auto-focus" is unbelievable! Please clarify.
>
> Best regards,
> Core Imaging Facility Staff
> NTU