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Zeiss or Olympus

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RICHARD BURRY

Re: Zeiss or Olympus

Vladimir

We have a new Olympus FV 1000 MPE with Spectral Physics Mai Tai DeepSee laser with 25x 1.02 N.A. 2 mm working distance objective The system has four non-descanned detectors and a forward second harmonic generation detector. The single photon capability uses four lasers, two spectral detectors and one filter detector. We are happy with our system and have penetration almost 1 mm for imaging fluorescent proteins in tissue. The SHG system is great and adds imaging for collagen with out having to label it.

Dick

----- Original Message -----
From: George McNamara <[hidden email]>
Date: Saturday, March 20, 2010 7:32 pm
Subject: Re: Zeiss or Olympus
To: [hidden email]

> Find the money for an LSM 780 (NIH S10 maximum limit this year
> is
> $600K and you can get a nicely equipped 780 within that amount -
> not
> counting the MP laser, you can always move one of your lasers
> over).
> If not, get an LSM710. The FV1000 is old technology.
>
> Full disclosure: I manage an LSM 710 and just hosted a Zeiss
> educational workshop featuring a 780 (www.zeiss.com/zoyc).
>
> At 08:18 AM 3/9/2010, Vladimir Gukassyan wrote:
> >Dear List Members,
> >
> >We're making a selection between Zeiss 710 and Olympus FV1000
> for the
> >multiphoton imaging.
> >I would be thankful for all user experience cases and
> suggestions on
> >this matter.
> >
> >Thank you in advance.
> >With regards,
> >Vladimir
> >
> >---------------------------------
> >Vladimir Ghukasyan
> >Confocal and Multiphoton Imaging Core,
> >Neuroscience Research Center
> >University of North Carolina
> >115 Mason Farm Rd., Bld. 245, 7 Fl.
> >Chapel Hill, NC 27599-7250
> >Tel.: +1 919 966 5807
>
>
>
>
>
>
>
> George McNamara, Ph.D.
> Image Core Manager
> Analytical Imaging Core Facility
> University of Miami, Miller School of Medicine
> Miami, FL 33136
> [hidden email]
> [hidden email]
> 305-243-8436 office
> http://www.sylvester.org/AICF (Analytical Imaging Core Facility)
> http://www.sylvester.org/AICF/pubspectra.zip (the entire 2000+
> spectra .xlsx file is in the zip file)
> http://home.earthlink.net/~geomcnamara
>
>
> --
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Richard W. Burry, Ph.D.
Department of Neuroscience, College of Medicine
Campus Microscopy and Imaging Facility, Director
The Ohio State University
Associate Editor, Journal of Histochemistry and Cytochemistry
277 Biomedical Research Tower
460 West Twelfth Avenue
Columbus, Ohio 43210
Voice 614.292.2814 Cell 614.638.3345 Fax 614.247.8849

Armstrong, Brian

Re: Zeiss or Olympus

In reply to this post by George McNamara

I would echo what George states below except that I would like to
suggest that it really depends upon what you mean by multiphoton
imaging. If you want to have a swiss-army-knife system that does many
things for many people then I would start with George's advice from
below. However, if you want to do excellent intra-vital imaging you will
want to look at the LSM710 Examiner and the Prairie Technologies Ultima.

As is often the case, it's hard to have one system that accomplishes all
your imaging needs.

Cheers,

Brian Armstrong PhD
Light Microscopy and Digital Imaging
Beckman Research Institute
Neuroscience
X62872

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of George McNamara
Sent: Saturday, March 20, 2010 1:28 PM
To: [hidden email]
Subject: Re: Zeiss or Olympus

Find the money for an LSM 780 (NIH S10 maximum limit this year is
$600K and you can get a nicely equipped 780 within that amount - not
counting the MP laser, you can always move one of your lasers over).
If not, get an LSM710. The FV1000 is old technology.

Full disclosure: I manage an LSM 710 and just hosted a Zeiss
educational workshop featuring a 780 (www.zeiss.com/zoyc).

At 08:18 AM 3/9/2010, Vladimir Gukassyan wrote:

>Dear List Members,
>
>We're making a selection between Zeiss 710 and Olympus FV1000 for the
>multiphoton imaging.
>I would be thankful for all user experience cases and suggestions on
>this matter.
>
>Thank you in advance.
>With regards,
>Vladimir
>
>---------------------------------
>Vladimir Ghukasyan
>Confocal and Multiphoton Imaging Core,
>Neuroscience Research Center
>University of North Carolina
>115 Mason Farm Rd., Bld. 245, 7 Fl.
>Chapel Hill, NC 27599-7250
>Tel.: +1 919 966 5807

George McNamara, Ph.D.
Image Core Manager
Analytical Imaging Core Facility
University of Miami, Miller School of Medicine
Miami, FL 33136
[hidden email]
[hidden email]
305-243-8436 office
http://www.sylvester.org/AICF (Analytical Imaging Core Facility)
http://www.sylvester.org/AICF/pubspectra.zip (the entire 2000+
spectra .xlsx file is in the zip file)
http://home.earthlink.net/~geomcnamara

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Andreas Bruckbauer

Re: Zeiss or Olympus

In reply to this post by RICHARD BURRY

We just got the new version of the Fluoview software for our Olympus multiphoton and it has now the laser control buld in so that multi track with different wavelength is possble. Furthermore it can do hard disk recording for long movies.

best wishes

Andreas

-----Original Message-----
From: RICHARD BURRY <[hidden email]>
To: [hidden email]
Sent: Mon, 22 Mar 2010 0:17
Subject: Re: Zeiss or Olympus

Vladimir

Dick

----- Original Message -----
From: George McNamara <[hidden email]>
Date: Saturday, March 20, 2010 7:32 pm
Subject: Re: Zeiss or Olympus
To: [hidden email]

> Find the money for an LSM 780 (NIH S10 maximum limit this year
> is
> $600K and you can get a nicely equipped 780 within that amount -
> not
> counting the MP laser, you can always move one of your lasers
> over).
> If not, get an LSM710. The FV1000 is old technology.
>
> Full disclosure: I manage an LSM 710 and just hosted a Zeiss
> educational workshop featuring a 780 (www.zeiss.com/zoyc).
>
> At 08:18 AM 3/9/2010, Vladimir Gukassyan wrote:
> >Dear List Members,
> >
> >We're making a selection between Zeiss 710 and Olympus FV1000
> for the
> >multiphoton imaging.
> >I would be thankful for all user experience cases and
> suggestions on
> >this matter.
> >
> >Thank you in advance.
> >With regards,
> >Vladimir
> >
> >---------------------------------
> >Vladimir Ghukasyan
> >Confocal and Multiphoton Imaging Core,
> >Neuroscience Research Center
> >University of North Carolina
> >115 Mason Farm Rd., Bld. 245, 7 Fl.
> >Chapel Hill, NC 27599-7250
> >Tel.: +1 919 966 5807
>
>
>
>
>
>
>
> George McNamara, Ph.D.
> Image Core Manager
> Analytical Imaging Core Facility
> University of Miami, Miller School of Medicine
> Miami, FL 33136
> [hidden email]
> [hidden email]
> 305-243-8436 office
> http://www.sylvester.org/AICF (Analytical Imaging Core Facility)
> http://www.sylvester.org/AICF/pubspectra.zip (the entire 2000+
> spectra .xlsx file is in the zip file)
> http://home.earthlink.net/~geomcnamara
>
>
> --
> BEGIN-ANTISPAM-VOTING-LINKS
> ------------------------------------------------------
>
> Teach CanIt if this mail (ID 1015274920) is spam:
> Spam:
> https://antispam.osu.edu/b.php?i=1015274920&m=906833d4ce50&c=sNot spam: https://antispam.osu.edu/b.php?i=1015274920&m=906833d4ce50&c=n
> Forget vote:
> https://antispam.osu.edu/b.php?i=1015274920&m=906833d4ce50&c=f---
> ---------------------------------------------------
> END-ANTISPAM-VOTING-LINKS
>

Richard W. Burry, Ph.D.
Department of Neuroscience, College of Medicine
Campus Microscopy and Imaging Facility, Director
The Ohio State University
Associate Editor, Journal of Histochemistry and Cytochemistry
277 Biomedical Research Tower
460 West Twelfth Avenue
Columbus, Ohio 43210
Voice 614.292.2814 Cell 614.638.3345 Fax 614.247.8849