Zeiss piezo stage

[Magali Mondin]

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Dear all,



To make a long story short, we have on our platform 2 Zeiss LSM 710 and 780
(NLO). They were equipped with piezo stages (Piezo Fine Focusing Stage),
for specific XZ applications. As we have some issues to use them for these
specific applications, we would like to have general experience feedbacks
with these Zeiss piezo stages installed on scanning confocals.



Particularly concerning the ease of use, re-positioning reproducibility to
himself and to the z limits defined with the micro-z, lifetime of the
system installed on a very used microscope…



And of course we are particularly interested with any experience with Z
studies, either in XYZ mode followed by orthogonal projections, or using
the XZ mode!



Thanks for your Help!



Mag



*Magali Mondin, Ph.D.*
 ------------------------------

*Plateforme d'imagerie PRISM*
iBV – CNRS UMR 7277 – INSERM 1091 -- UNS
Parc Valrose
06108 NICE cedex 2

* tel: [phone] fax :[phone]*
mail : [hidden email]*; *[hidden email]
 ------------------------------

[George McNamara]

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Hi Magali,

Hopefully you have a resonant scanner to complement your Piezo type
stage(s). R.S. enables as fast scanning in XZ as in XY. 14 full frames
per second on the Leica SP5 I used to manage in Miami ... I believe the
new SP8 RS is even faster, and acquiring fewer rows enables higher
speed, something like 100 fps for 50 rows (the SP5 also had a rotator
mirror to change scanning orientation wrt specimen).

If you do not have R.S., carefully evaluate your acquisition parameters
for XZ to get the performance you need. I suggest using reflection mode
on a coverglass, that is, the air-coverglass-air interfaces, timelapse
mode, to evaluate Z-stability (reflection gives a much brighter signal -
and photostable - compared to fluorescence ... on LSM7x0's need to click
a checkbox to enable bright reflection ... also, use pinhole 1 A.U.).
Changing Z-range should not affect how the specimen looks. If there is a
bidirectional Z option you can ask whether the surfaces are in the same
pixels in each direction.

Please publish what you find. It sounds like Alby can use more
manuscripts for M.R.T (page charges, not gold open access without more
charges). Also consider Journal of Biomolecular Techniques,
http://jbt.abrf.org/  -- "gold" (immediate) open access and no
publication charge and Rich Cole or Claire Brown might be the light
microscopy handling editors?.


Enjoy,

George

On 6/10/2014 3:47 AM, Magali Mondin wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
>
>
> To make a long story short, we have on our platform 2 Zeiss LSM 710 and 780
> (NLO). They were equipped with piezo stages (Piezo Fine Focusing Stage),
> for specific XZ applications. As we have some issues to use them for these
> specific applications, we would like to have general experience feedbacks
> with these Zeiss piezo stages installed on scanning confocals.
>
>
>
> Particularly concerning the ease of use, re-positioning reproducibility to
> himself and to the z limits defined with the micro-z, lifetime of the
> system installed on a very used microscope…
>
>
>
> And of course we are particularly interested with any experience with Z
> studies, either in XYZ mode followed by orthogonal projections, or using
> the XZ mode!
>
>
>
> Thanks for your Help!
>
>
>
> Mag
>
>
>
> *Magali Mondin, Ph.D.*
>   ------------------------------
>
> *Plateforme d'imagerie PRISM*
> iBV – CNRS UMR 7277 – INSERM 1091 -- UNS
> Parc Valrose
> 06108 NICE cedex 2
>
> * tel: [phone] fax :[phone]*
> mail : [hidden email]*; *[hidden email]
>   ------------------------------
>
>    

--



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/26/

[Dan Focht]

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*****

Magali


If it is a old "very used microscope" you might want to consider having all the lubricants in the stage and focusing system cleaned and replaced with fresh lubricants to prevent slow settling or lagging that occurs due to viscosity changes in old lubricants before relying on such a sensitive translation device.

Dan





On Jun 10, 2014, at 4:47 AM, Magali Mondin wrote:

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*****

Dear all,



To make a long story short, we have on our platform 2 Zeiss LSM 710 and 780
(NLO). They were equipped with piezo stages (Piezo Fine Focusing Stage),
for specific XZ applications. As we have some issues to use them for these
specific applications, we would like to have general experience feedbacks
with these Zeiss piezo stages installed on scanning confocals.



Particularly concerning the ease of use, re-positioning reproducibility to
himself and to the z limits defined with the micro-z, lifetime of the
system installed on a very used microscope…



And of course we are particularly interested with any experience with Z
studies, either in XYZ mode followed by orthogonal projections, or using
the XZ mode!



Thanks for your Help!



Mag



*Magali Mondin, Ph.D.*
------------------------------

*Plateforme d'imagerie PRISM*
iBV – CNRS UMR 7277 – INSERM 1091 -- UNS
Parc Valrose
06108 NICE cedex 2

* tel: [phone] fax :[phone]*
mail : [hidden email]*; *[hidden email]
------------------------------


Dan Focht
Bioptechs, Inc.
3560 Beck Rd.
Butler, PA 16002
www.bioptechs.com
P: [phone]
F: [phone]
[hidden email]

[Magali Mondin]

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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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*****

Thanks for your answers.

More precisely, when we talk about "used" microscope it means that we have
a lot of users on both systems (LSM 710 and LSM 780) and we wonder about
the resistance of the stage over time ;)
For our fast XZ applications (live dynamics), on both LSM 710 and LSM 780
driven by ZEN, we observe distorsions of the images when compared to XZ
acquisitions done with the microscope Z. Does anyone tried these kind of
acquisitions with the piezo stage mounted on either 710 or 780 microscopes?

Thanks!

Mag




2014-06-10 17:01 GMT+02:00 Dan Focht <[hidden email]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Magali
>
>
> If it is a old "very used microscope" you might want to consider having
> all the lubricants in the stage and focusing system cleaned and replaced
> with fresh lubricants to prevent slow settling or lagging that occurs due
> to viscosity changes in old lubricants before relying on such a sensitive
> translation device.
>
> Dan
>
>
>
>
>
> On Jun 10, 2014, at 4:47 AM, Magali Mondin wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear all,
>
>
>
> To make a long story short, we have on our platform 2 Zeiss LSM 710 and 780
> (NLO). They were equipped with piezo stages (Piezo Fine Focusing Stage),
> for specific XZ applications. As we have some issues to use them for these
> specific applications, we would like to have general experience feedbacks
> with these Zeiss piezo stages installed on scanning confocals.
>
>
>
> Particularly concerning the ease of use, re-positioning reproducibility to
> himself and to the z limits defined with the micro-z, lifetime of the
> system installed on a very used microscope…
>
>
>
> And of course we are particularly interested with any experience with Z
> studies, either in XYZ mode followed by orthogonal projections, or using
> the XZ mode!
>
>
>
> Thanks for your Help!
>
>
>
> Mag
>
>
>
> *Magali Mondin, Ph.D.*
> ------------------------------
>
> *Plateforme d'imagerie PRISM*
> iBV – CNRS UMR 7277 – INSERM 1091 -- UNS
> Parc Valrose
> 06108 NICE cedex 2
>
> * tel: [phone] fax :[phone]*
> mail : [hidden email]*; *[hidden email]
> ------------------------------
>
>
> Dan Focht
> Bioptechs, Inc.
> 3560 Beck Rd.
> Butler, PA 16002
> www.bioptechs.com
> P: [phone]
> F: [phone]
> [hidden email]
>

[Mark Cannell-2]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

For fast xz scanning the hydraulic coupling due to the immersion fluid between the slide and objective can cause distortion especially if the slide is not well supported and/or the coverslip not well fixed to the slide.

HTH

Mark


On 11/06/2014, at 10:43 am, Magali Mondin <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Thanks for your answers.
>
> More precisely, when we talk about "used" microscope it means that we have
> a lot of users on both systems (LSM 710 and LSM 780) and we wonder about
> the resistance of the stage over time ;)
> For our fast XZ applications (live dynamics), on both LSM 710 and LSM 780
> driven by ZEN, we observe distorsions of the images when compared to XZ
> acquisitions done with the microscope Z. Does anyone tried these kind of
> acquisitions with the piezo stage mounted on either 710 or 780 microscopes?
>
> Thanks!
>
> Mag
>
>
>
>
> 2014-06-10 17:01 GMT+02:00 Dan Focht <[hidden email]>:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Magali
>>
>>
>> If it is a old "very used microscope" you might want to consider having
>> all the lubricants in the stage and focusing system cleaned and replaced
>> with fresh lubricants to prevent slow settling or lagging that occurs due
>> to viscosity changes in old lubricants before relying on such a sensitive
>> translation device.
>>
>> Dan
>>
>>
>>
>>
>>
>> On Jun 10, 2014, at 4:47 AM, Magali Mondin wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> Post images on http://www.imgur.com and include the link in your posting.
>> *****
>>
>> Dear all,
>>
>>
>>
>> To make a long story short, we have on our platform 2 Zeiss LSM 710 and 780
>> (NLO). They were equipped with piezo stages (Piezo Fine Focusing Stage),
>> for specific XZ applications. As we have some issues to use them for these
>> specific applications, we would like to have general experience feedbacks
>> with these Zeiss piezo stages installed on scanning confocals.
>>
>>
>>
>> Particularly concerning the ease of use, re-positioning reproducibility to
>> himself and to the z limits defined with the micro-z, lifetime of the
>> system installed on a very used microscope…
>>
>>
>>
>> And of course we are particularly interested with any experience with Z
>> studies, either in XYZ mode followed by orthogonal projections, or using
>> the XZ mode!
>>
>>
>>
>> Thanks for your Help!
>>
>>
>>
>> Mag
>>
>>
>>
>> *Magali Mondin, Ph.D.*
>> ------------------------------
>>
>> *Plateforme d'imagerie PRISM*
>> iBV – CNRS UMR 7277 – INSERM 1091 -- UNS
>> Parc Valrose
>> 06108 NICE cedex 2
>>
>> * tel: [phone] fax :[phone]*
>> mail : [hidden email]*; *[hidden email]
>> ------------------------------
>>
>>
>> Dan Focht
>> Bioptechs, Inc.
>> 3560 Beck Rd.
>> Butler, PA 16002
>> www.bioptechs.com
>> P: [phone]
>> F: [phone]
>> [hidden email]
>>

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology &  Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]