Guosheng,
as already stated, it could be a timing problem. So maybe the hardware
was not ready yet while starting the software. If it appears more often
or always, there might be three reasons - as far as I know.
a) The X-Y motor of the pinhole is getting old and takes longer to move
the pinhole into its initial position. After a certain time, a sensor
checks if the pinhole arrived at this position. So the pinhole might
arrive after the sensor readout. In this case, the service might
exchange the drive.
b) The sensor which checks the position has a defect. Can be exchanged
as well.
c) The saved position for the pinhole is too far away from the central
one, and it takes too long to move the pinhole there. You can check the
initial position in the AIM software. Initiate it, and go directly to
Maintain > Pinhole. Have a look at the X-Y values, are the far away from
0? If yes, and the pinhole is still nicely adjusted in terms of
Z-resolution, a service engineer can reset the physical position of the
pinhole in the scan-head.
In the end it could be a combination of problems. So if the problem
persists, contact your service.
Good luck,
Michael
Holly Aaron schrieb:
> Try Julio's approach 2-3 times, and if that doesn't work, you can try
> using CANCheck (CANCHK), which is in the AIM folder or in the Tools
> Folder within AIM or in the CANCHK folder within AIM, depending on the
> version of software you have. Depending on the version of CANCHK, there
> are ways to reset the firmware. If the below protocol does not work for
> you, the Zeiss support can walk you through it, too, or give me a call
> and let me know which version you have and I can walk you through the
> reset process. Very rarely, it could be that the pinhole is broken or
> lost power or something, and then you'll need a service call, but it is
> good to try this first.
>
> Best,
> Holly
>
> Holly L Aaron
>
> Molecular Imaging Center
>
> Cancer Research Laboratory
>
> 251 Life Sciences Addition #2751
>
> Berkeley, CA 94720-2751
>
> 510.642.2901
>
> 510.642.5741 FAX
>
>
[hidden email] <mailto:
[hidden email]>
>
>
http://imaging.berkeley.edu>
>
>
> Julio Vazquez wrote:
>> =
>> PH1 refers to pinhole 1, the pinhole on front of the META detector.
>> The error message most likely indicates that the software lost contact
>> with the pinhole. Typically, you can fis this by quitting the software
>> and restarting it. If that doesn't work, quit software, shut down and
>> restart system, and restart software. Zeiss softwasre is a bit picky,
>> and you may need to wait a little after you power ON the system and
>> before you start the LSM software to allow all devices to initiate
>> properly. If you still see problems after that, you may need a service
>> visit.
>>
>> Julio.
>>
>> --
>> Julio Vazquez,
>> Fred Hutchinson Cancer Research Center
>> 1100 Fairview Ave. N., mailstop DE-512
>> Seattle, WA 98109-1024
>>
>>
http://www.fhcrc.org/>>
>>
>> On Aug 12, 2009, at 12:55 PM, G.Liu wrote:
>>
>>> Hello Listers,
>>>
>>> A error message pops up this morning in our Zeiss 510Meta: "Object
>>> PH1 time
>>> out". It seems it is associated with ChS channel (Meta detector)
>>> since ChS
>>> is not working.
>>>
>>> Does anyone out there have any idea on it? Any suggestion to fix it
>>> would be
>>> greatly appreciated!
>>>
>>> Thanks,
>>>
>>> Guosheng
>>> --
>>> View this message in context:
>>>
http://n2.nabble.com/Zeiss510--error-log%3A-Object-PH1%2C-Time-out-tp3433598p3433598.html>>> Sent from the Confocal Microscopy List mailing list archive at
>>> Nabble.com.
>>
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