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Dear Jeff,
Disclosure: I am working with Abbelight (shared PhD student) and we have
published together several articles.
We have the SAFe360 instrument since last year. Abbelight has worked a lot
to add features and the setup is performing quite well. A distinctive
feature is the possibility to image a large filed-of-view in STORM even
with reasonable laser (~300 mW) thanks to the ASTER widefield scanning
method explained in this very recent preprint
https://www.biorxiv.org/content/10.1101/2020.05.08.083774v1.article-metrics.
200 x 200 µm area is useful for our imaging of axonal networks, see here:
https://www.youtube.com/watch?v=XxNQrVsgjJ4. Another feature that we are
excited about is the spectral demixing SMLM that allows 2-3
colors simultaneous imaging using far-red fluorophores (AF647, CF660,
CF680) split on two camera by a well-chosen dichroic, with assignment of
each fluorophore based on the balance of intensities in each low/high
spectral band image. IN our tests it allows multi-channel STORM with a
similar quality is all channels, something that is difficult to achieve
with fluorophores of different colors (488/568/647).
Regarding cost, I can't really say as this can vary quite a bit, maybe
Abbelight can comment on it or contact you for more information.
Hope this helps,
Christophe
--
Christophe Leterrier
NeuroCyto lab
INP CNRS UMR 7051
Aix Marseille University, France
On Tue, 12 May 2020 at 05:12, Jeff Spector <
[hidden email]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Greetings,
> We are currently stuck at home and planning our next few big
> acquisitions for the lab.
> The safe60 system from abbelight looks quote promising. Can anyone with
> experience on this system give me their feedback, as well as an
> approximate cost?
> Thanks,
> -Jeff
>
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