acetone fixation and RNA detection

[Sylvie Le Guyader]

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Hi listers

Does anyone know if it is possible to detect RNAs on cells fixed with acetone?
The protocol used on the cells before staining unfortunately requires acetone.

Med vänlig hälsning / Best regards

Sylvie

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Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website<https://ki.se/en/bionut/welcome-to-the-lci-facility>
Follow our microscopy blog!<http://microscopykarolinska.se/>




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[Miller,Douglas R]

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Hi Sylvie,

As long as it's free of RNAse, it shouldn't affect the RNA.
https://onlinelibrary.wiley.com/doi/full/10.1046/j.1365-294x.1999.00795.x?sid=nlm%3Apubmed

Best,

Doug
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Subject: acetone fixation and RNA detection

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Hi listers

Does anyone know if it is possible to detect RNAs on cells fixed with acetone?
The protocol used on the cells before staining unfortunately requires acetone.

Med vänlig hälsning / Best regards

Sylvie

@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader, PhD
Live Cell Imaging Facility Manager
Karolinska Institutet- Bionut Dpt
Blickagången 16,
Room 7362 (lab)/7840 (office)
14157 Huddinge, Sweden
mobile: [phone]
LCI website<https://urldefense.proofpoint.com/v2/url?u=https-3A__ki.se_en_bionut_welcome-2Dto-2Dthe-2Dlci-2Dfacility&d=DwIFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=3i4rxt62ZtjRndK4HyoTIA&m=oI3ygEur58jv9SxG7a4T9xrs3gxR2ILghrGWoA6d8FU&s=vTCrQiyXaDoJ3E1kbyqhEQ0aky8ViCr9xeo14_vFnos&e= >
Follow our microscopy blog!<https://urldefense.proofpoint.com/v2/url?u=http-3A__microscopykarolinska.se_&d=DwIFAw&c=sJ6xIWYx-zLMB3EPkvcnVg&r=3i4rxt62ZtjRndK4HyoTIA&m=oI3ygEur58jv9SxG7a4T9xrs3gxR2ILghrGWoA6d8FU&s=bThobCUTA8YhETWucAfDJHX_8QS_StiGW7aEuH5w7Ns&e= >




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