advanced perfusion systems

[Vitaly Boyko-2]

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 Dear All,
We are looking for an advanced perfusion system with up to 8 pumps that can be programmed to monitor input and output media volume, flow rate, and more, and compatible with the Nikon NES software.Any recommendations will be greatly appreciated.
Best regards,
VitalyMSKCC/MCCF

   
 

[Romain Laine]

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Dear Vitaly,
Depending on the types of experiments that you have in mind and your
budget, you may want to consider using our NanoJ-Fluidics system.
https://www.nature.com/articles/s41467-019-09231-9
It's an open-source, cheap perfusion system for on-microscope treatments of
sample.

It's really easy to multiplex and you can control volumes and rates but
there's some limitations in terms of the complexity of simultaneous
perfusion.
I have not tried interfacing it with NIS but there may be a way via serial.
It's also not a big commitment to build a couple and give it a shot.

I hope this helps !

Romain

On Tue, Sep 10, 2019 at 9:35 PM Vitaly Boyko <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>  Dear All,
> We are looking for an advanced perfusion system with up to 8 pumps that
> can be programmed to monitor input and output media volume, flow rate, and
> more, and compatible with the Nikon NES software.Any recommendations will
> be greatly appreciated.
> Best regards,
> VitalyMSKCC/MCCF
>
>
>
>

[Vitaly Boyko-2]

*****
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*****

 Hi Romain,
thank you very much for the useful information.In simpler terms, we plan to use ididi or labtek II 4-well dishes with 170 um bottom, with four inlets and four outlets, and add fixed amount of a drug/dye, test different drugs at different concentrations, etc. in the range of 100-300 ul, remove or replace the media, be able to control (calibrate) the flow rate, volume, etc.Most of perfusion systems handle single well dishes (e.g. delta T dish with inserts which is not practical along basic drug assays), with one inlet/one outlet, based on a gravity flowNikon NIS may work via serial (it functions via TTL pulses).
How much effort would it be to integrate it into the Nikon NIS and have it running smoothly?
Best,
Vitaly



    On Wednesday, September 11, 2019, 03:13:31 AM EDT, Romain Laine <[hidden email]> wrote:  
 
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*****

Dear Vitaly,
Depending on the types of experiments that you have in mind and your
budget, you may want to consider using our NanoJ-Fluidics system.
https://www.nature.com/articles/s41467-019-09231-9
It's an open-source, cheap perfusion system for on-microscope treatments of
sample.

It's really easy to multiplex and you can control volumes and rates but
there's some limitations in terms of the complexity of simultaneous
perfusion.
I have not tried interfacing it with NIS but there may be a way via serial.
It's also not a big commitment to build a couple and give it a shot.

I hope this helps !

Romain

On Tue, Sep 10, 2019 at 9:35 PM Vitaly Boyko <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>  Dear All,
> We are looking for an advanced perfusion system with up to 8 pumps that
> can be programmed to monitor input and output media volume, flow rate, and
> more, and compatible with the Nikon NES software.Any recommendations will
> be greatly appreciated.
> Best regards,
> VitalyMSKCC/MCCF
>
>
>
>

 

[Romain Laine]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Vitaly,
What you describe seems possible with NanoJ-Fluidics (aka Pumpy on
Twitter). The Arduino controlling the pumps are themselves controlled via
serial commands, and I believe that NIS elements can be used to send serial
commands to connected devices (does anyone have experience with doing
that?).
Re calibration and volumes, this can be done with the software we already
have.

If you need specific help setting it up, please do not hesitate to get in
touch ([hidden email])!

Romain




On Wed, Sep 11, 2019 at 8:35 PM Vitaly Boyko <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>  Hi Romain,
> thank you very much for the useful information.In simpler terms, we plan
> to use ididi or labtek II 4-well dishes with 170 um bottom, with four
> inlets and four outlets, and add fixed amount of a drug/dye, test different
> drugs at different concentrations, etc. in the range of 100-300 ul, remove
> or replace the media, be able to control (calibrate) the flow rate, volume,
> etc.Most of perfusion systems handle single well dishes (e.g. delta T dish
> with inserts which is not practical along basic drug assays), with one
> inlet/one outlet, based on a gravity flowNikon NIS may work via serial (it
> functions via TTL pulses).
> How much effort would it be to integrate it into the Nikon NIS and have it
> running smoothly?
> Best,
> Vitaly
>
>
>
>     On Wednesday, September 11, 2019, 03:13:31 AM EDT, Romain Laine <
> [hidden email]> wrote:
>
>  *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Vitaly,
> Depending on the types of experiments that you have in mind and your
> budget, you may want to consider using our NanoJ-Fluidics system.
> https://www.nature.com/articles/s41467-019-09231-9
> It's an open-source, cheap perfusion system for on-microscope treatments of
> sample.
>
> It's really easy to multiplex and you can control volumes and rates but
> there's some limitations in terms of the complexity of simultaneous
> perfusion.
> I have not tried interfacing it with NIS but there may be a way via serial.
> It's also not a big commitment to build a couple and give it a shot.
>
> I hope this helps !
>
> Romain
>
> On Tue, Sep 10, 2019 at 9:35 PM Vitaly Boyko <
> [hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> >  Dear All,
> > We are looking for an advanced perfusion system with up to 8 pumps that
> > can be programmed to monitor input and output media volume, flow rate,
> and
> > more, and compatible with the Nikon NES software.Any recommendations will
> > be greatly appreciated.
> > Best regards,
> > VitalyMSKCC/MCCF
> >
> >
> >
> >
>
>