air bubble

[Pieter Vanden Berghe]

Dear all,

we've got an upright microscope with a 20x water dipping lens that we use for Ca imaging in tissues. Much like a patch clamp experiment we insert a perfusion pipette between the lens and the tissue to administer drugs etc. The solutions are all oxygenated CO2/bicarbonate buffered. Unfortunately it happens (too often) that an bubble (air/O2/CO2?) gets trapped under the lens, which obviously perturbs the image to an extent that measuring is no longer possible.

I'm sure some of you have experienced similar problems. Any thought on how to solve this (in a simple way)?

Best regards,

Pieter

[Franco Del Principe]

Dear Pieter

For our acute cerebellar thick slices we used a custom-designed chamber
with so-called bubble traps. That is, the tubing with the oxygenated
perfusion terminated within a small circular trough at the side of the
chamber. Bubbles escaped from this trough while the perfusate streamed
through a slit below the liquid surface into the main chamber where the
slice was placed. I am sure there are commercial versions available.

Cheers

Franco

Pieter Vanden Berghe wrote:

> Dear all,
>
> we've got an upright microscope with a 20x water dipping lens that we
> use for Ca imaging in tissues. Much like a patch clamp experiment we
> insert a perfusion pipette between the lens and the tissue to administer
> drugs etc. The solutions are all oxygenated CO2/bicarbonate buffered.
> Unfortunately it happens (too often) that an bubble (air/O2/CO2?) gets
> trapped under the lens, which obviously perturbs the image to an extent
> that measuring is no longer possible.
>
> I'm sure some of you have experienced similar problems. Any thought on
> how to solve this (in a simple way)?
>
> Best regards,
>
> Pieter
>
>
>

--
       ___      ___   _______
      /  /     /  /  /  ____/
     /  /     /  /  /  /___
    /  /     /  /  /___   /
   /  /___  /  /  ____/  /
  /______/ /__/  /______/             Dr. Franco Del Principe
------------------------------------------------------------
LIFE IMAGING SERVICES GmbH           Fon     [phone]
Efringerstrasse 79                   Fax     [phone]
CH-4057 Basel, Switzerland           Mob     [phone]
E-mail  [hidden email]    Web   http://www.lis.ch

[PattyJansma]

fyi


At 07:01 AM 9/2/2009, you wrote:

>Dear Pieter
>
>For our acute cerebellar thick slices we used a custom-designed chamber
>with so-called bubble traps. That is, the tubing with the oxygenated
>perfusion terminated within a small circular trough at the side of the
>chamber. Bubbles escaped from this trough while the perfusate streamed
>through a slit below the liquid surface into the main chamber where the
>slice was placed. I am sure there are commercial versions available.
>
>Cheers
>
>Franco
>
>Pieter Vanden Berghe wrote:
>>Dear all,
>>we've got an upright microscope with a 20x water dipping lens that we use
>>for Ca imaging in tissues. Much like a patch clamp experiment we insert a
>>perfusion pipette between the lens and the tissue to administer drugs
>>etc. The solutions are all oxygenated CO2/bicarbonate buffered.
>>Unfortunately it happens (too often) that an bubble (air/O2/CO2?) gets
>>trapped under the lens, which obviously perturbs the image to an extent
>>that measuring is no longer possible.
>>I'm sure some of you have experienced similar problems. Any thought on
>>how to solve this (in a simple way)?
>>Best regards,
>>Pieter
>>
>
>--
>       ___      ___   _______
>      /  /     /  /  /  ____/
>     /  /     /  /  /  /___
>    /  /     /  /  /___   /
>   /  /___  /  /  ____/  /
>  /______/ /__/  /______/             Dr. Franco Del Principe
>------------------------------------------------------------
>LIFE IMAGING SERVICES GmbH           Fon     [phone]
>Efringerstrasse 79                   Fax     [phone]
>CH-4057 Basel, Switzerland           Mob     [phone]
>E-mail  [hidden email]    Web   http://www.lis.ch