anti GFP gold conjugates/Qdots

[Kevin Mackenzie]

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Does anyone know of a commercial source for anti GFP gold conjugates for fluorescent and electron microscopy?

 

Also has anyone any experience of using Qdots for correlative microscopy?

 

thanks

 

Kevin

The University of Aberdeen is a charity registered in Scotland, No SC013683.

[Ignatius, Mike]

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi kevin,

 

Not sure if Tom Deerinck or Mark Ellisman at UCSD are on this listserve but they are the pros at working with Qdots for LM/EM.  Any of their outstanding papers should be of use. 

 

e.g. Sosinsky GE, Giepmans BN, Deerinck TJ, Gaietta GM, Ellisman MH.

Markers for correlated light and electron microscopy.

Methods Cell Biol. 2007;79:575-91. Review.

 

Anti-GFP - Qdot conjugates could be made from the ITK (innovator's tool kit) Quantum Dots which are conjugatible and anti-GFP - both available from Molecular Probes/Invitrogen.  For example sku# Q21341MP, 525 QDot nanocrystals are ready for EDAC linkage to amines etc.   Search terms 'ITK Qdots' gets you the entire listing.  We haven't made the direct conjugate. 

 

Mike Ignatius,

 

Molecular Probes/Invitrogen

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Mackenzie, Kevin S.
Sent: Thursday, May 22, 2008 5:32 AM
To: [hidden email]
Subject: anti GFP gold conjugates/Qdots

Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

 

Does anyone know of a commercial source for anti GFP gold conjugates for fluorescent and electron microscopy?

 

Also has anyone any experience of using Qdots for correlative microscopy?

 

thanks

 

Kevin

The University of Aberdeen is a charity registered in Scotland, No SC013683.