antibodies to fluorescent proteins from SCAM

[Roger Phillips]

Dear Microscopy Users (if you don't want to get this kind of information,
let me know and I will drop you from my list)

For those of you who work with fluorescent proteins and have wondered about
antibody specificity against these proteins here is some useful information:

From Confocal List server replies:

'We have been working a little with an anti-RedFP from Rockland
(600-401-379) to
probe for Cherry and the Living Colors JL-8 anti-GFP for GFP.
On westerns they both look specific.
Marc D Green
[hidden email]
Pombe.net'

'As noted, DsRed, mRFP1, mCherry, mStrawberry, and tdTomato are derived from
a common source.

Of these, the rabbit polyclonal anti-DsRed antibody from BD, #632397
recognizes all except tdTomato by western blotting. It will also IP these
proteins.

Similarly, GFP, EGFP, CFP, Cerulean, YFP, Citrine, and Venus are derived
from a common source, as are the common monomeric variants of the same (the
A206K mutation is most typical).

We have confirmed that the JL-8 monoclonal from BD recognizes Venus,
Cerulean, CFP, YFP, mCFP, mYFP, and EGFP by western blotting. We have not
seen it crossreact against DsRed, mRP1, or mCherry. For IPs we use the Abcam
antibody 'ab290', which works well for EGFP, YFP, mYFP, CFP, and mCFP, and
does not capture DsRed, mRFP1, or mCherry.

Good luck,
Steve
Stephen C. Bunnell, Ph.D.
Assistant Professor
Tufts University Medical School
Department of Pathology
Jaharis Bldg., Room 512
150 Harrison Ave.
Boston, MA 02111

Phone: [phone]
Fax:   [phone]
Email: [hidden email]'

Cheers,
Roger

Dr Roger Guy Phillips
Centre for Advanced Microscopy,
University of Sussex
School of Life Sciences
John Maynard Smith Building
Falmer, Brighton & Hove
BN1 9QG
United Kingdom

phone:[phone]
fax: [phone]
email: [hidden email]
room:2C9 (ext 7585)/lab 4C2 (ext 2734)