bleaching of Alexa488 fluorophore after 750nm two photon scanning

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david anchel david anchel
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bleaching of Alexa488 fluorophore after 750nm two photon scanning

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Hi there, I'm wondering if anyone knows of a photostable fluorophore that does
not excite with two-photon excitation at 750nm.  I am bleaching with this
wavelength, but I find that in the region I bleach the Alexa 488 signal is also
bleached as well, no matter how low I turn down the two-photon power.

thanks,
dave anchel