blood vessel labeling

[manojshree]

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Hello George,

Thanks. There is one more point, we are using 2-p micorscope and are using
green and red dye for neurons/astrocyets. We are looking for blue emission
range dye for good separation of blood vessels.

Excitation is either 800nm or 950nm in case of Gfp Mice.

Thanks,
manoj

On Sun, Aug 5, 2012 at 9:02 AM, George McNamara
<[hidden email]>wrote:

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> *****
>
> Hi Manoj,
>
> Volocity or Imaris or Huygens (for latter see Alby's reply) can probably
> do what you want. Fiji ImageJ might be able to.
>
> FARSIGHT Toolkit has already done it:
>
> http://www.farsight-toolkit.**org/wiki/FARSIGHT_Toolkit<http://www.farsight-toolkit.org/wiki/FARSIGHT_Toolkit>
>
> Associative image analysis: a method for automated quantification of 3D
> multi-parameter images of brain tissue. </pubmed/18294697> Bjornsson CS,
> Lin G, Al-Kofahi Y, Narayanaswamy A, Smith KL, Shain W, *Roysam B*.
>
> J Neurosci Methods. 2008 May 15;170(1):165-78. PMID: 18294697
>
> http://www.ncbi.nlm.nih.gov/**pmc/articles/PMC2700351/**figure/F4/<http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2700351/figure/F4/>
>
> Badri Roysam and colleagues have a lot more papers using FARSIGHT, above
> was the first.
>
> While I'm thinking about it - request to Fiji ImageJ/ImageJ2 organizers:
> Please incorporate FARSIGHT into Fiji.
>
>
> Blood vessel labeling:
>
> Consider DiA, ~450 nm excitation, ~590 nm emission (so "blue" with respect
> to excitation).
>
> Best to learn how to label blood vessels with lipophilic dyes by starting
> out with DiI:
>
> Li Y, Song Y, Zhao L, Gaidosh G, Laties AM, Wen R. Direct labeling and
> visualization of blood vessels with lipophilic carbocyanine dye DiI. Nat
> Protoc. 2008;3(11):1703-8. PubMed PMID: 18846097
>
> Plan BV:
>
> Consider a BV421-antibody conjugate, such as anti-CD31 (anti-PECAM). For
> more on BV421 and its siblings, see
>
> http://www.biolegend.com/**brilliantviolet<http://www.biolegend.com/brilliantviolet>
>
> Plan H: you should be able to label all the blood vessel nuclei with
> Hoechst or other DNA counterstains. If you want to label just the
> endothelial cell nuclei, might be able to do this (somewhat) selectively by
> using low concentration and perfusion incubation time.
>
> George
>
>
>
> On 8/5/2012 5:40 AM, Manoj Jaiswal wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> > *****
> Hello friends,
>
> I need to buy a 3D quantification software to analyze data in large
> z-stack.
>
> Stack is going to be complex where we need to visualize blood vessesl,
> artery and neurons and astocytes.  I dont know which is the best software
> available in the market. Any suggestion would be great.
>
> Hello folks,
>
> I am looking for a dye for blood vessels labeling in the blue emission
> range. Any suggestion. Cascade blue did not work well in our hand and i did
> not find any dextean conjugates in blue color.
> Thanks,
>
> Manoj
>