camera reccomendations
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Greetings,
We're in the process of procuring a new microscope system and I wanted to ask this list about cameras. We currently use andor ixon du-897 cameras, but have seen that there is a bigger chip versions of this camera, and that hamamatsu also have a big chip emccd, and apparently roper has the new prime95B which they claim can be used for single molecule work. I was wondering if anyone has any experience doing single molecule imaging with cameras other than our andor 897's. While on paper the big-chip versions seem to be just as good, I'd like to know first hand experience of people using them in the field... Also interested to know if there are major differences between the andor and hamamatsu cameras. I noticed that the big chip emccds have 13um pixels as opposed to the 16um of the 512x512 versions which i guess would be important when imaging single molecule fluorescence. Anyone with any experience with these cameras, your opinions would be greatly appreciated... thanks, -Jeff |
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Hi Jeff, if you want to keep the optics like they are, I mean everything is optimized for large pixels, then you know the emCCD cameras from Andor and Hamamatsu
and Photometrics. Then the only alternative would be right now the new prime95B sCMOS camera with large pixels (11um pixel pitch). In case you consider changing the optics or if you look for larger resolution then you might want to test the other sCMOS cameras
being offered from PCO, Andor and Hamamatsu (6.5um pixel pitch). Whether they are suited or not certainly depends of the amount of light coming out of your single molecule experiments. Since all the sCMOS cameras with the smaller pixel size are successfully
applied to localization microscopy applications (PALM, STORM, dSTORM, GSD) they might do as well. If you are not sure, I am convinced that all of the companies will help with free trials. with best regards, Gerhard ___________________________ Dr. Gerhard Holst Science & Research PCO AG Donaupark 11 93309 Kelheim, Germany fon +49 9441 2005 36 fax +49 9441 2005 20 mob +49 172 711 6049 www.pco.de Von: Confocal Microscopy List [mailto:[hidden email]]
Im Auftrag von Jeff Spector ***** To join, leave or search the confocal microscopy listserv, go to:
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Greetings, We're in the process of procuring a new microscope system and I wanted to ask this list about cameras. We currently use andor ixon du-897 cameras, but have seen that there is a bigger chip versions of this
camera, and that hamamatsu also have a big chip emccd, and apparently roper has the new prime95B which they claim can be used for single molecule work. I was wondering if anyone has any experience doing single molecule imaging with cameras other than our andor
897's. While on paper the big-chip versions seem to be just as good, I'd like to know first hand experience of people using them in the field... Also interested to know if there are major differences between the andor and hamamatsu cameras. I noticed that the big chip emccds have 13um pixels as opposed to the 16um of the 512x512 versions which i guess
would be important when imaging single molecule fluorescence. Anyone with any experience with these cameras, your opinions would be greatly appreciated... thanks, -Jeff |
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