chambers for volatile organics

[Evangelos Gatzogiannis]

  I've been working lately on confocal microscopy with very volatile and
toxic organics.  The chambers described today are quite useful
(Matek...) for cell culture and cell imaging; however, the solvents I've
been working with lately readily attack plastic and rubber.  I would
need a chamber made of glass or fused silica, with a thickness at the
bottom comparable to a 0, 1, or 1.5 standard microscope coverslip for an
inverted microscope, and Luer Locks or similar pipette access ports made
of Teflon or with Teflon plugs.  I haven't found a supplier yet, if any
vendor here or any of you know of a vendor that makes any type of
chamber with glass only and with PTFE plugs that would be terrific.

Best,
Evangelos
Harvard CNS

[Megan Nicholson]

What solvents are you using?

Megan

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Evangelos
Sent: Wednesday, June 10, 2009 12:26 PM
To: [hidden email]
Subject: chambers for volatile organics

  I've been working lately on confocal microscopy with very volatile and
toxic organics.  The chambers described today are quite useful
(Matek...) for cell culture and cell imaging; however, the solvents I've
been working with lately readily attack plastic and rubber.  I would
need a chamber made of glass or fused silica, with a thickness at the
bottom comparable to a 0, 1, or 1.5 standard microscope coverslip for an
inverted microscope, and Luer Locks or similar pipette access ports made
of Teflon or with Teflon plugs.  I haven't found a supplier yet, if any
vendor here or any of you know of a vendor that makes any type of
chamber with glass only and with PTFE plugs that would be terrific.

Best,
Evangelos
Harvard CNS

[Beat Ludin]

Our chambers have originally been developed for work with volatile
anesthetics and are made of stainless steel and glass. Two Viton
O-rings (can be replace by silicone, if you like) are used to seal
the chamber but the exposed surface is minimized, so even if the
Viton or silicone should only be partially compatible with your
solvents, the chamber should still work fine. You can even coat the
O-rings with PTFE, if required. The perfusion in-/outlets are made of
steel too, and interface directly to PTFE tubing.

This is another shameless commercial plug, of course :-)

Beat

At 19:25 10-06-2009, you wrote:

>  I've been working lately on confocal microscopy with very volatile
> and toxic organics.  The chambers described today are quite useful
> (Matek...) for cell culture and cell imaging; however, the solvents
> I've been working with lately readily attack plastic and rubber.  I
> would need a chamber made of glass or fused silica, with a
> thickness at the bottom comparable to a 0, 1, or 1.5 standard
> microscope coverslip for an inverted microscope, and Luer Locks or
> similar pipette access ports made of Teflon or with Teflon
> plugs.  I haven't found a supplier yet, if any vendor here or any
> of you know of a vendor that makes any type of chamber with glass
> only and with PTFE plugs that would be terrific.
>
>Best,
>Evangelos
>Harvard CNS

[Erik_LVBT]

I would also take a look at what the German company GeSiM (www.gesim.com)
could do for you: they produce a whole range of microfluidic products, also
custom-made, at a good price. They have some experience of combining
organics with bio too.

No present commercial ties, although I once did represent them as a
distributor.

Best regards,

Erik von Stedingk

-----Ursprungligt meddelande-----
Från: Confocal Microscopy List [mailto:[hidden email]] För
Evangelos
Skickat: den 10 juni 2009 19:26
Till: [hidden email]
Ämne: chambers for volatile organics

  I've been working lately on confocal microscopy with very volatile and
toxic organics.  The chambers described today are quite useful
(Matek...) for cell culture and cell imaging; however, the solvents I've
been working with lately readily attack plastic and rubber.  I would
need a chamber made of glass or fused silica, with a thickness at the
bottom comparable to a 0, 1, or 1.5 standard microscope coverslip for an
inverted microscope, and Luer Locks or similar pipette access ports made
of Teflon or with Teflon plugs.  I haven't found a supplier yet, if any
vendor here or any of you know of a vendor that makes any type of
chamber with glass only and with PTFE plugs that would be terrific.

Best,
Evangelos
Harvard CNS
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18:08:00

[vb-2]

Dear All,

I would greatly appreciate if you could provide me with the list of
objectives with the measured flatness of field in 100 nm steps within the
working distance of the objective (i.e. accurately!!! measured by the
manufacturer - I might guess that German companies could be more
"responsible & accurate" than other non-German ones, like Nikon, especially
when the product is to be delivered for the "broad" American Market).

Thank you for your help,

Vitaly

NCI-Frederick,
301-846-6575